29. Cell Imaging Techniques

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MIT 7.016 Introductory Biology, Fall 2018
Instructor: Adam Martin
View the complete course: ocw.mit.edu/7-016F18
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Professor Martin introduces cell imaging techniques, which are tools that allow biologists to observe what's going on living cells. He goes over several types of microscopy and discusses how each deals with the challenges of resolution and contrast.
License: Creative Commons BY-NC-SA
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Пікірлер: 12

@CodeJeffo 2 жыл бұрын

Regarding the resolution formula. I believe one has to consider rather the wavelength of the emission light not the excitation light. If you take the excitation wavelength that formula tells you to what smaller possible spot you can focus that light to. Vice versa if you have 2 GFP fluorophores emitting at certain wavelength this is what's important not the excitation light. Otherwise we would not observe worse resolution the further red we go with fluorophores.

@jakeeutis5035 4 жыл бұрын

What a neat technique, no wonder he got a Nobel prize

@anjaligupta6180 3 жыл бұрын

true

@tasoth6624 3 жыл бұрын

agree

@carolynlambert8797 2 жыл бұрын

took intro to biology at tidewater community college from here go anywhere!

@MaeLSTRoM1997 2 жыл бұрын

I think it's funny that the view count decays rapidly from lecture 1 through to 29, and then suddenly there's a spike of interest on lecture 30 followed by another decay. I wonder if it had anything to do with the current state of the world..

@Altas576 Жыл бұрын

Yes I was noticing that too! I almost think of it as "weeding out" those who are truly passionate about the subject versus those who are not. Shoutout to the people who watch the underrated lectures

@luvisacigarette8 3 жыл бұрын

Is there an explanation of multiphoton microscopy?

@leothecrafter4808 3 жыл бұрын

A very short one for you: You take light of a higher wavelenght, usually NIR and use very strong but short pulses to cause non linear optical effects in the sample. This causes the NIR to be converted to VIS, which is then picked up by a PMT. Because these non linear effects need a very high energy density, it only occurs in the focal plane, no pinhole required. Also the NIR penetrates tissue very well, so you can image thick slices or even live animals.

@luvisacigarette8 3 жыл бұрын

@@leothecrafter4808 Thank you!