1) Introduction 1:07 --Limitations of usual transmited-light microscopy 1:08 --Main developments for biological imaging: 1:41 .....Fluorescent labeling 1:42 .....Super resolutuion techniques 3:32 .....3D imaging of thick tissue 4:45 --Conventional fluorescence microscopy 5:59 --Fluorescence confocal microscopy 6:45 --Multiphoton microscopy 8:19 .....1 photon excitation 10:08 .....2 photon excitation 10.32 ...........2 photon excited fluorescence 2PEF 11:26 .....3 photon excitation 12:30 --Excitation of multiphoton processes 13:14

2) Implementation of multiphoton microscopy 14:53 --Multiphoton microscope setup 14:55 .....Laser scanning excitation 15:00 .....Polarization adjustment 16:25 .....Objective lens 16:39 .....Backward detection 17:28 .....Forward detection 18:15 --F vs B detection 18:30 --Multiphoton microscopy resolution 20:00 --Advantages of multiphoton microscopy 23.06 .....in depth imaging in biological tissues 23:10 .....reduced phototoxicity / photobleaching 32:02 .....Variety of non-linear optical processes 36:29 .....endogenous multiphoton sources of contrast 38:23 --Model tissue cornea 39:46 --Multiphoton imaging of unstained cornea 41:27 .....Endogenous 2PEF .....Endogenous SHG 46:13 --Physics of SHG .....exogenous SHG signals 51:02 --Collagen 54:59 .....Collagen SHG response 57:18 .....Collagen SHG imaging 58:57 .........Example fibrotic pathologies 1:02:22 --Myosin SHG signals 1:06:03 .....Myosin SHg imaging 1:08:03 --Microtubule SHG signal 1:08:27

This is just awesome, thanks for sharing.

The lecture was very good. But I hope there is an English subtitle.

1:11:17 THG