Рет қаралды 5,982
This lecture focuses on nonlinear optical microscopy (also called multiphoton microscopy) and its application in biological tissues. It is organized as follows: 1. Introduction 2. Implementation of multiphoton microscopy Setup Spatial resolution Imaging depth Other features 3. Endogenous multiphoton signals Two-Photon Excited Fluorescence (2PEF) Second Harmonic Generation (SHG) Third Harmonic Generation (THG) Coherent Raman Scattering (CRS) 4. Some examples of recent developments Quantitative SHG imaging of collagen Polarization-resolved SHG microscopy 5. Conclusion