Quantitative real time PCR (qPCR)

Рет қаралды 242,622

Animated biology With arpan

6 жыл бұрын

Пікірлер: 127

@morvolio-5223 10 ай бұрын

This is that guy on youtube that explains ambiguous concepts very well, which you are searching for. Amazing!

@animatedbiologywitharpan 10 ай бұрын

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@rosegott3849 2 жыл бұрын

Really well done, thanks! I wish someone had walked me through qPCR this well when I first started running them!

@animatedbiologywitharpan 2 жыл бұрын

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@animatedbiologywitharpan 2 жыл бұрын

I gave different playlists on different techniques , so don't forget to check out all of that

@emmaadams8797 3 жыл бұрын

Using this to prep for a mock interview tomorrow. You're a lifesaver. Thank you!

@animatedbiologywitharpan 3 жыл бұрын

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@rutovincentk 4 жыл бұрын

This is the best explanation,I have ever seen I can now understand Real time qPCR

@animatedbiologywitharpan 4 жыл бұрын

Vincent Kibet please share

@jaydebpal1818 2 жыл бұрын

very good overview. can you please make a detailed one on melt curve analysis and primer designing

@sadiqaisha4057 2 жыл бұрын

Thank you so much Arpan! I've never understood qPCR like I did in this 8 mins video!

@animatedbiologywitharpan 2 жыл бұрын

Please share my channel link with your friends and help me to reach big audience

@Universe12355 7 ай бұрын

Love your channel and the way you make difficult concepts easy. I had a question about primer property checking using Primer-BLAST. I have a primer that contains degeneracy/wobbles and I want to check its properties such as Tm, self-complementarity, self 3’ complementary using the primer- blast… However, when I use the primer Blast and search, it states that “ambiguity letters other than N are not allowed in the primer sequence” So that forced me to replace all the degenerate/wobble letters with N. Would that still make the property results correct? Am I still getting the right answers if they are replaced with N? Thank you so much, I would really appreciate some insight on this, as I can not find an answer anywhere.

@cutieaadi9599 7 ай бұрын

If I get a annealing temperature 66.4 and got single peak of melting curve, can I use that annealing temperature? While the Melting curve temperature range is 65_95’c ….what you suggest?

@riseillyramos852 2 жыл бұрын

Omg i love you! Really wish my professor explained this well.

@animatedbiologywitharpan 2 жыл бұрын

Lots of love from India 🇮🇳. Please share my channel link with your friends and help me to reach big audience

@kavitharachaiah8313 Жыл бұрын

Clear explanation. But do add some more text in your slides, like principle of the technique & lable each & every component in the slides. So that it will be much useful to us. Thank you.

@animatedbiologywitharpan Жыл бұрын

Really glad to know it was useful. Please share my channel with friends. You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me

@mdraismdsaman47mdsaman80 Жыл бұрын

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@mdraismdsaman47mdsaman80 Жыл бұрын

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@moleculartraveler2481 2 жыл бұрын

This video is really well explained and helpful

@animatedbiologywitharpan 2 жыл бұрын

Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8& You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me

@lizhang2849 5 жыл бұрын

than you man, it helps

@animatedbiologywitharpan 5 жыл бұрын

Share among friends and help me to reach big audience

@ardranellikunnel7247 2 жыл бұрын

Is sybr green specifically attached with the target gene?

@animatedbiologywitharpan 2 жыл бұрын

No no it can bind to any double stranded dna, but the pcr will selectively amplify the target gene so in the reaction tube there will be huge quantity of target with which sybr green can bind

@ZannaInnominata0126 3 жыл бұрын

Great job, dude!

@animatedbiologywitharpan 3 жыл бұрын

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@Teejay_shan 4 ай бұрын

Sir I am checked hiv RNA quantitative pcr test result is target not dedicat meaning is negative hiv ? This is conclusive result ? Pls answer me😒

@usrao2148 2 жыл бұрын

You are great teacher, !!!

@animatedbiologywitharpan 2 жыл бұрын

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@DrVRManoj 4 жыл бұрын

Very good lecture !

@animatedbiologywitharpan 4 жыл бұрын

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@sheratajayasinghe448 7 ай бұрын

The explanation was really good. N easy to understand. Thank you very much most of my doubts was cleared ❤

@animatedbiologywitharpan 7 ай бұрын

Watch the detailed video kzfaq.info/get/bejne/qq6Pot2Uq921j4U.htmlsi=Y78Ps-hNVHvNBjoM

@sristi_2360 3 жыл бұрын

Can qrt pcr be used to determine the exact number of viruses that have infected the sample?

@animatedbiologywitharpan 3 жыл бұрын

No …a plaque assay can determine that.

@gurralamohankumar5838 4 жыл бұрын

Thank u so muchhhh sir

@humanityfirst5228 2 жыл бұрын

You are gonna touch the sky 🌙

@animatedbiologywitharpan 2 жыл бұрын

Thanks for your compliments. Please share my channel link with your friends and help me to reach big audience

@crlearning3460 Жыл бұрын

Hi. Is there a cell type qPCR only uses? Or is available to use with all cells?

@animatedbiologywitharpan Жыл бұрын

For all cell types

@gurralamohankumar5838 4 жыл бұрын

Please make a video on 3' RACE and 5' RACE

@SaumyaSharma123 2 жыл бұрын

What is the need to do qpcr over pcr sir?

@animatedbiologywitharpan 2 жыл бұрын

Watch this video to learn kzfaq.info/get/bejne/ldZ9dtqDqreWYn0.html

@animatedbiologywitharpan 2 жыл бұрын

Please share my channel link with your friends and help me to reach big audience

@vamsikrishnagokarla5207 2 жыл бұрын

@@animatedbiologywitharpan this link is not opening sir

@amir91095 2 жыл бұрын

with PCR you collect RFU data once the 40 cycles are complete vs qPCR you collect RFU data across all cycles; hence why q stands for quantitative

@sachinsarraf6708 5 жыл бұрын

Can it is use for rabies detaction...

@moussaeshra6212 Жыл бұрын

What you mean by base line Like the first florescent ever or when it is above the threshold number?

@animatedbiologywitharpan Жыл бұрын

The baseline of the real-time PCR reaction refers to the signal level during the initial cycles of PCR, usually cycles 3 to 15, in which there is little change in fluorescent signal. The low-level signal of the baseline can be equated to the background or the “noise” of the reaction

@moussaeshra6212 Жыл бұрын

@@animatedbiologywitharpan thanks alot dr

@harshithaanand1742 3 жыл бұрын

thank you so much was very very helpful

@animatedbiologywitharpan 3 жыл бұрын

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@mtctafadzwa2318 3 жыл бұрын

Well understood thank you

@animatedbiologywitharpan 3 жыл бұрын

Please share my channel link with your friends and help me to reach big audience

@dimpabhalru8063 4 ай бұрын

sir abhi window period mai hu sir actually next month mai canada jaaa rha hu pr m glti se hepatitis C ke contact mai agya hu … sir confirm nhi horha….. pls btao sir kuch 25 febuary ko needle use ki thi hep c postive ldke ki sir fr 10 din baad hcv pcr rna quantitative test krwaya …. Usme not detected aya … sir plss btao next test kab karwau ki accurate aajaye jldi mne canada jana h.. pls help sir🙏🙏🙏

@user-dj1wu7ye5f 2 жыл бұрын

He is a legend ....ARPAN is a LEGEND ....

@animatedbiologywitharpan 2 жыл бұрын

I am not sure that if he is an ( whatsoever you have mentioned ). I know him personally, he had to do a lot of struggle in his life . Thanks for your compliments though.

@user-dj1wu7ye5f 2 жыл бұрын

@@animatedbiologywitharpan rectified ..felt sorry

@ayat4522 3 жыл бұрын

sukriya , good video

@animatedbiologywitharpan 3 жыл бұрын

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@kocharkhasro387 4 жыл бұрын

great

@animatedbiologywitharpan 4 жыл бұрын

Please share among friends and help me to reach big audience

@monigopi2460 Жыл бұрын

I cant find the Value analysis video

@animatedbiologywitharpan Жыл бұрын

It's a paid content now

@reyfranzheiropacardo5524 3 жыл бұрын

thank you

@animatedbiologywitharpan 3 жыл бұрын

If you like the explanation please share my channel link with your friends and help me to reach big audience

@spajder75 4 жыл бұрын

Very nice video, but it would be great if there was subtitles

@animatedbiologywitharpan 4 жыл бұрын

Thanks for your feedback

@kiplimosimon1429 11 ай бұрын

Excellent work

@animatedbiologywitharpan 11 ай бұрын

Please help us by sharing our videos with your friends

@fathimasulaiman5360 4 жыл бұрын

Best explanation about real time pcr.... Really good 😍😍😍😍😍

@animatedbiologywitharpan 4 жыл бұрын

Do subscribe to my other channel kzfaq.info/love/4IpyopsGWSjaPACNTZLuqg

@animatedbiologywitharpan 4 жыл бұрын

Please share among friends and help me to reach big audience

@fathimasulaiman5360 4 жыл бұрын

@@animatedbiologywitharpan of course sir👍👍👍👍👍👍

@harshithamohan4074 2 жыл бұрын

Thank you

@animatedbiologywitharpan 2 жыл бұрын

Please share my channel link with your friends and help me to reach big audience

@ominibioentrance6458 4 ай бұрын

Good explanations.......what if the ct value of control and treated sample are same say 26 for both. Does it mean that the transcript level is same in both.

@animatedbiologywitharpan 4 ай бұрын

Absolute CT value does not mean any thing the delta CT value is the key measure.

@ominibioentrance6458 4 ай бұрын

Thank you......

@leonweoow2746 2 жыл бұрын

is mlpa a qpcr type?

@animatedbiologywitharpan 2 жыл бұрын

Could you please tell me the full form of mlpa

@sadiarajpoot7433 3 жыл бұрын

Can anyone suggest me a book from which I can learn about Real time pcr

@alb4079 3 жыл бұрын

Why did you like the comment, but didn't suggest any literature to this person?

@danaahmed8158 3 жыл бұрын

Introduction to biotechnology. By William J. Thieman and Michael A. Palladino.

@sadiarajpoot7433 3 жыл бұрын

@@danaahmed8158 Thank you

@danaahmed8158 3 жыл бұрын

@@sadiarajpoot7433 😊😊😊

@lakindublin 3 жыл бұрын

Dorak's real time PCR

@hemantk345 6 жыл бұрын

Awesome bro nice

@animatedbiologywitharpan 6 жыл бұрын

Thanks glad it helped you

@hemantk345 6 жыл бұрын

I used to watch your videos bro this year i have secured AIR 256 but now I am pursuing biotechnology 2nd year i hope at the final year rank will be up

@animatedbiologywitharpan 6 жыл бұрын

Sure you would do great....stay optimistic

@hemantk345 6 жыл бұрын

Thank u bro😇

@hemantk345 6 жыл бұрын

Bro will u make a video on NMR (conceptual lecture)

@prajwalgosavi2001 Жыл бұрын

Sir i recently done test rna pcr the sample was collected in heparin tube will this affect my result? Or the person carrying this test how will he know about inhibitors ?it was qualitative test.

@animatedbiologywitharpan Жыл бұрын

It's very difficult to tell, it depends what you want to detect ultimately

@prajwalgosavi2001 Жыл бұрын

@@animatedbiologywitharpan it was related to virus detection about sti it was sent to thyrocare lab?

@vaishnavidharmadhikari21 10 ай бұрын

Thank you sir

@animatedbiologywitharpan 10 ай бұрын

So nice of you. Please share my channel link with your friends and help me to reach big audiance

@W5nli 2 ай бұрын

How you got melt curve chart?

@animatedbiologywitharpan 2 ай бұрын

you can add melt curve after the standard PCR cycle

@W5nli 2 ай бұрын

@@animatedbiologywitharpan can you explain more how?

@W5nli 2 ай бұрын

@@animatedbiologywitharpan i use ncbl website

@fatimajahaf1412 3 жыл бұрын

is qpcr same as gradient pcr?

@animatedbiologywitharpan 3 жыл бұрын

No no gradient PCR is completely different from qPCR

@fatimajahaf1412 3 жыл бұрын

@@animatedbiologywitharpan what's the core difference please

@animatedbiologywitharpan 3 жыл бұрын

I will upload a video on that. Just wait till tomorrow.

@fatimajahaf1412 3 жыл бұрын

@@animatedbiologywitharpan is bio rad the only manufacturer of gradient real time pcr?

@ermionemariastavrouli8903 Жыл бұрын

thank god your channel exists ahha

@animatedbiologywitharpan Жыл бұрын

Please support our channel by using super thanks. Your 2$ donation would give us motivation to do more. Super thanks option is present below any of my videos, a heart sign with $ in it.