AutoSpill uses robust regression to identify and correct for spillover errors in flow cytometry data. The resulting compensation matrix will be as accurate as is possible given the quality of controls used. For best results, use single-stained cell controls prepared in exactly the same was as in the fully stained samples.
Publication:
www.nature.com/articles/s4146...
R package:
github.com/carlosproca/autospill
Integration into FlowJo:
docs.flowjo.com/flowjo/experi...