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Analytical Instrumentation - Raman Spectroscopy
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One may have heard about Raman effect quite a lot of times. In this video we are going to discuss the concept of Raman scattering first. But we also need to understand Rayleigh scattering first. Once the phenomenon of Raman scattering is explained, this video will discuss why and how the Raman effect takes place and how Raman scattering is generated. The viewer will also learn about the stokes lines and anti-stokes lines and how Raman scattering is a two photon process. Finally, the use of Raman effect in spectroscopy is discussed. This will help to perform qualitative and quantitative analysis on the sample under test.
The Raman scattering takes place due to inelastic collision between photons and electrons. The difference in energy between incident photon and emitted photon generates Raman lines. Depending on the emitted frequency, the lines generated are called as Stokes lines if their frequency is less than incident frequency of photons, if the frequency of emitted photons is greater than incident frequency, it is called as Anti-stokes lines. From the Raman spectra, one can determine the molecule in the sample by studying the stokes and anti-stokes lines. Similarly, from their intensity one can calculate the concentration of a particular sample.
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