Basics of chromatography | Chemical processes | MCAT

Basics of chromatography | Chemical processes | MCAT | Khan Academy

Рет қаралды 1,200,940

11 жыл бұрын

Understand the basic principles of different kinds of chromatography: paper, thin layer, column, size-exchange, ion exchange, affinity, HPLC, and. By Angela Guerrero. . Created by Angela Guerrero.
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Пікірлер: 273

@metoo2here 10 жыл бұрын

Hi..the video is great..but you have made a mistake with size exclusion chromatography..the molecules that are larger in size (the ones excluded from the beads due to larger size) are the first ones to elute out followed by the small, the smaller and the smallest..the reason being that the molecules which can enter the beads via the pores have a longer path and hence a longer transit time.

@5Youssif 7 жыл бұрын

upvote so others see

@iansteinke2414 6 жыл бұрын

That is a different concept in general. So im not entirely sure this applies. Correct me if im wrong, but in liquid chromatography size is a factor yes, but polarity is the main concern here.

@EDERDUDE 2 жыл бұрын

Correct

@oliviadenyer-dobrowolski7441 7 жыл бұрын

Shout out to you! Thank-you for your simple yet complex explanations because I completely understand this topic now and feel confident to write my extended response!

@TheSurvivalSecrets 10 жыл бұрын

That was extremely helpful, thanks!

@CORPSE77 6 жыл бұрын

* correction, it should say: in size- exchange chromatography, small particles get trapped in the column by the small beads, thus small particles are slower to move. I just read that in Kaplan MCAT biochem review.

@gemechubekele2546 8 жыл бұрын

6:28 In size exchange(exclusion) chromatography, aren't they the bigger molecules that move quicker than the smaller ones because the smaller ones have to travel in the beads?

@easylearning981 8 жыл бұрын

+Gemechu Bekele Yes.

@khadijahadam4387 8 жыл бұрын

Yup agree. In size exchange chromatography if the size is too big it wont pass through the beads instead it will flow through the column. Only small particles will flow through the beads hence, smaller particles is said to have higher retention time

@pranavvariava2685 7 жыл бұрын

emeg losota yes

@VincentStevenson 6 жыл бұрын

It's the same principle as DNA Gel electrophoresis, there's no limit to the length of DNA that can pass through the gel. Really long molecules will travel with smaller ones through the silicone, but much slower.

@ND-ji8dk 2 жыл бұрын

@@VincentStevenson no not same principle, larger DNA pieces travel less further due to interactions with agarose gel. whereas in SEC larger elute out first/travel faster as they do not get stuck in the holes of the bead

@bushraduti6780 10 жыл бұрын

extremely helpful :) love your voice :)

@pablolobo2070 6 жыл бұрын

Thank you, very informative!

@MrW0lfies 6 жыл бұрын

Thank you soooooo much. This will definitely help me with my lab

@EHBRod13 9 жыл бұрын

You totally sound like Alphonse Elric from Full Metal Alchemist: Brotherhood. Amazing video, by the way. Thank you.

@A_Dopamine_Molecule 6 жыл бұрын

You're absolutely right, I can't unhear it now :/

@firephoenix8192 6 жыл бұрын

Absolutely.

@kasondemulenga3472 6 жыл бұрын

Oh my God yes she does!

@ekhs2669 3 жыл бұрын

When your a weeb and u spent all the time watching anime and now is studying 1 hour before the exam

@kemjikaechebelem451 7 жыл бұрын

thank you so much those extremely helpful!

@AKASHVERMA-ok2ns 6 жыл бұрын

Soo beautiful voice And you explan clearly Thanx for sharing knowledge.... 🙂

@iqtidarrahman2148 9 жыл бұрын

I had no idea what it meant, but then I realised I did this at primary! Thanks

@coreyking212 8 жыл бұрын

That moment when a 4th grader becomes your teacher :P...

@remusomega 8 жыл бұрын

+Gemechu Bekele The moment you fail verbal reasoning because you can't take a joke

@sylvecakes8450 8 жыл бұрын

+Gemechu Bekele how do you know it is not a fake channel?

@nathanbrown4689 7 жыл бұрын

+Gemeche Bekele, that joke not only flew over you, it fucking soared past at fucking mach 6 lol

@sylvecakes8450 7 жыл бұрын

I did not type that it was my friend for reals

@saintofchelseathomascarlyl5713 6 жыл бұрын

it was my friend

@anousharahimzada5837 9 жыл бұрын

Thank you so much ! Have exams soon and this assisted me :)

@sadamalinimalaweera4528 3 жыл бұрын

Great video, super helpful thanks

@trilliumchang4127 8 жыл бұрын

The point on size chromatography is wrong. Larger size elutes faster as it can go around the beads, whilst smaller beads have greater retention as it has to go through the intriciate pores.

@peepdi 5 жыл бұрын

True that.

@peepdi 5 жыл бұрын

@@hargobindsingh3167 oh is it? Cause even we studied about the larger ones eluting fast.

@hargobindsingh3167 5 жыл бұрын

@@peepdi sorry was wrong here mixed a concept😅

@peepdi 5 жыл бұрын

@@hargobindsingh3167 it's completely fine 😁

@robertsanchez4316 5 жыл бұрын

This is indeed true. Smaller molecules travel through the porous beads thereby making their path to elute much larger than that of larger molecules. I am an advance biochemistry student lol

@estrellasolimar9019 7 жыл бұрын

thank you. this was very helpful.

@amanal-kabbani2745 2 жыл бұрын

Thanks for the helpful video..I've made a simple at-home experiment involving paper chromatography but instead of drawing a dot..I drew a line with a black pen used for whiteboard..and it separated to 3 colors as I remember..and I used a mixture of water/ethanol to get the best separation!

@jansenlimawan8415 6 жыл бұрын

Thanks, very helpful.

@hanan2184 2 жыл бұрын

this video made it make so much sense THANK YOU

@IIAmzazZ 10 жыл бұрын

thanks helped alot!

@jasroop_sandhu 8 жыл бұрын

Beautifully Explained!

@Mikeek7 5 жыл бұрын

this was helpful. thank you!

@joshprichard9049 9 жыл бұрын

me to thanks got a test common up and this helped me :)

@nonoooooooooo1 9 жыл бұрын

u just saved my life thank you so much

@albertchen2020 4 жыл бұрын

Aww, her voice is so cute

@Chem4students 3 жыл бұрын

khan academy is my favorite channel. by the inspiration of this channel i started my channel covering all topics regarding pharmaceutical analysis & biochemistry. i did video on basics on chromatography.

@safeyyasofy5515 9 жыл бұрын

Thanks girl ! The best here 👏👏👏👏👏👍👍👍👍👍😙

@ashrafazman2859 5 жыл бұрын

this video help me so much... tq :)

@lalithkishore4249 7 жыл бұрын

very nice... thanks a lot for the video. ..

@DuongNguyen-ni9gz Жыл бұрын

excellently explained !

@beckytonn5527 8 жыл бұрын

Love your pictures. They are GREAT: )

@vivaanmaheshwari9852 Жыл бұрын

yes you are right and like

@imShifa 5 жыл бұрын

Thank you.

@thilinaalagiyawanna3680 3 жыл бұрын

Thank You very much

@aztec4194 6 жыл бұрын

Nice video really helped before exam thanks you so much

@aronrohithsingh2730 Жыл бұрын

Thank you so much

@nerdykityplantchild6301 10 жыл бұрын

This helped for science olympaid thanks!!

@legendarypanda424 4 жыл бұрын

Wait! Were you doing crime busters?

@badranmohamed5772 8 жыл бұрын

Than you very much for the help. the way you explained paper chromatography made it extremely simple. Again, thank you for such a video

@ruoyingzheng1680 Жыл бұрын

thank you for the great content.

@ibrahimkhoja 3 жыл бұрын

Thanks a lot 🙏

@nancy8202 2 жыл бұрын

Very very helpful ❤️

@gracysingh1737 3 жыл бұрын

Thank you !! Extremely helpful

@prit0012 2 жыл бұрын

our comments look pretty young out here 🤣.once upon a time Khan Academy used to be a very popular educational channel

@NK_2542_ Жыл бұрын

Great video.. more information in least time..✨✨✨

@sujanashwath9173 7 ай бұрын

This video is awesome ❤️‍🔥❤️‍🔥❤️‍🔥, I understood completely

@DuyenLe-kf7gj 4 жыл бұрын

Thank you so much for making the video. If possible, could you please change the font color in the future? As It is quite difficult to read your note toward the end of the video. Thank you again for your time

@kimdean2034 2 жыл бұрын

I love it 😍

@georgefloyd5078 Жыл бұрын

her voice is so soothing

@NAGAVERSE 5 жыл бұрын

4:32 COMMENTATORS SHOUTING : W...W...E.... Tables Ladders and Chairs TLC.

@emanabo-elmaaty8931 3 жыл бұрын

Fantastic ❤

@mounabaziz5906 3 жыл бұрын

Thank youu

@kristak.1625 5 жыл бұрын

everyone is either 1) sexualizing the speaker 2) criticizing and comparing the speaker to Sal... what does that even say about you guys? Lovely video, they're just jealous you understand chromatography and they don't.

@swanqilcooledition6666 4 жыл бұрын

Thanks this video helped me a lot!

@abdullahrahman9883 3 жыл бұрын

kewl

@elizavethmogrovejo2472 5 жыл бұрын

Helping me out for my regents today ☹️✅

@jore354 9 жыл бұрын

I think that you have it backwards in Size Exclusion Chromatography. The larger molecules can travel faster because they are not distracted by the other paths that can be taken to reach the end of the column.

@chriskoperniak784 8 жыл бұрын

+Joseph Landavaso No, she's right - the larger molecules travel slower

@naomik5213 8 жыл бұрын

+Chris Koperniak in size exclusion chromatography the large molecules elute quicker. The small ones get stuck in the beads. She is wrong.

@Bhupender855 6 жыл бұрын

Most underrated channel

@trilliumchang4127 8 жыл бұрын

Should probably mention most often for HPLC the silica gel is bound to a nonpolar group, so the more polar group elutes first v. all other techniques the more nonpolar group elutes first.

@Nymeria0 5 жыл бұрын

Doesn't that depend on the solvent that is used? In most cases, it would be polar solvent, hence the most polar compound would elute first.

@MrElectricSkittles 8 жыл бұрын

x1.5

@thefattyfatty1 8 жыл бұрын

+MrElectricSkittles Expert call right here

@mertarican5456 5 жыл бұрын

thx

@ryusworld2701 3 жыл бұрын

Hi yall

@michaelladenice 3 жыл бұрын

ye i really got annoyed and changed the playback speed

@muhammadrizwan3309 Жыл бұрын

nice work keep it up

@bansalpharmacycollege 7 жыл бұрын

very good

@lauren9004 9 ай бұрын

Interesting. Seem to be a few accuracy issues though. But it's a good video

@hasnathassan922 7 жыл бұрын

very nice

@hafsa1698 9 жыл бұрын

What happened to Sal's voice? 0_o

@WaddiaS 8 жыл бұрын

It's a KA spin-off

@lovelybias 8 жыл бұрын

+ChimChim I just scroll the page to see some of the comments idk why and I saw your current profile pic... BTS is following me even in my hw ;o;~

@nusaibahhussain9975 7 жыл бұрын

Ikr?

@audreyandremington5265 3 жыл бұрын

He started listening to Jo Jo Siwa and then his voice changed. It's sad. Don't listen to Jo Jo Siwa, kids.

@vetaya4783 7 жыл бұрын

Very good and simple explanation,thanks :))

@suhanapandey2749 3 жыл бұрын

In paper chromatography, the stationary phase is not the paper but water adsorbed into the paper

@OMNI_INFINITY 11 ай бұрын

That sounds like evalion. Would be pretty cool if she is doing chemistry now.

@xxxyyyyzzzzzzz 4 жыл бұрын

The stationary phase is actually water trapped between the cellulose fibres of the paper.

@TitusSupremus 8 жыл бұрын

10/10 ign most intrtuiging thing ever

@57.zoonabatool61 3 жыл бұрын

Can u plz make a tutorial on ascending paper chromatographyy

@mangatons 8 жыл бұрын

you should choose a better pallette of colours, some couldnt read what you wrote in the hplc, like me.

@tahsinrahman1668 7 жыл бұрын

so, the capillary action will take place in both?

@sylvecakes8450 8 жыл бұрын

Good drawings, but needs to concentrate and remember the speech

@TheChessRobloxian 11 ай бұрын

Bro this video helped a lot with my science assingment in Year 7!

@Philippine_Navyist16 9 ай бұрын

same

@TheChessRobloxian 9 ай бұрын

@@Philippine_Navyist16 I got a B+ for this video! I could've gotten an A if I hadn't gone over time!

@Philippine_Navyist16 9 ай бұрын

@@TheChessRobloxian todya is teachers day and no school, i dont know if my science teacher will approve this tom. Wisj me luk

@TheChessRobloxian 9 ай бұрын

@@Philippine_Navyist16 wdym ur science teacher won't approve? anyways good luck.

@Philippine_Navyist16 9 ай бұрын

@@TheChessRobloxian yay he aprovves

@ellerose1844 5 жыл бұрын

Does anyone have some videos I can use for science Olympiad

@floraacademics1195 7 жыл бұрын

kindly add true expt. which would be easy to understand

@sramtrain 8 жыл бұрын

where is this on the website?

@mukarrambutt453 3 жыл бұрын

In paper chromatography, the stationary phase is not paper but the liquid that is bonded upon it.

@hiraktailor1916 6 жыл бұрын

I m in love with your voice😀😀😆 And yes this was very useful thanks!!!

@emon8506 6 жыл бұрын

danke

@tirivokureangel4984 5 жыл бұрын

Nice

@mekhicampbell3226 2 жыл бұрын

Thx ima make ricen now

@epic8924 2 жыл бұрын

Came here from the video too

@ilaiyarajaips6919 7 жыл бұрын

good

@ishu9176 8 жыл бұрын

There are two principles on chromatography~ 1) Adsorption 2) 2 phases (Stationary and moving) Am I right?

@akaruiorenji5287 7 жыл бұрын

Ishu 1) adsorption 2) partition

@ishu9176 7 жыл бұрын

Akarui Orenji Oh yeah thanks :3

@georgemalpus7634 6 жыл бұрын

Ishu Cool

@roheenamurad2375 4 жыл бұрын

Stationary Phase in case of paper chromatography is liquid, because it has molecules of Water.

@sexyscientist 7 жыл бұрын

You are explaining GC as if it is fractional distillation.

@KonradKustosik 10 жыл бұрын

beautiful voice.

@yarakharam5343 6 жыл бұрын

are fucking kidding me she sound slike a 7 year old boy with a clogged nose. You must be some fucking omega-virgin.

@TheRayll 6 жыл бұрын

and this is why i love reading comments

@Abdullah-cj2mh 6 жыл бұрын

Nice Student idk why but "some kind of fucking omega-virgin" made my day

@TheRayll 6 жыл бұрын

ikr

@yarakharam5343 6 жыл бұрын

ty m8 I really felt it while typing

@edwinthekkinen9319 9 жыл бұрын

Cute voice.

@yashdeshpande249 9 жыл бұрын

Cute voice

@zakariajony4193 7 жыл бұрын

Munna sir's advice.

@preetimanoth1814 6 жыл бұрын

What is cpillary action

@Ersair 10 жыл бұрын

5/5, would watch again

@amyz9170 9 жыл бұрын

is her nose blocked or something? but good video

@gregorytaylor8809 2 жыл бұрын

wow thanks pushed my grade from an f to an A*. I'm gonna now major in chemical engineering to make the fortnite chug jug real

@princessvirina1649 5 жыл бұрын

just to clarify things out, this is paper chromatography right????????

@vijaykumar-bp4xb 3 жыл бұрын

is lipid molecule separated with the help of gas exchange chromatography?????????????

@theodoregoujon4017 7 жыл бұрын

so which substance is more polar? The one closer to the stationary or mobile phase?

@SantosAl 7 жыл бұрын

Theodore Goujon. depends on the the type of stationary phase/mobile phase you use.

@louiezhao2243 8 жыл бұрын

cute voice

@kidsandkids5687 2 жыл бұрын

Brief, precise, nice and short, although there is an error in size CrGphy. Excellent job 👍

@landoncochrane1030 2 жыл бұрын

Hi fbi personnel I'm not makeing ricen I would die doing it and I have no uses for it

@soham85e52 4 жыл бұрын

HELLO!