Biochemical rate calculation using Beers Law
Рет қаралды 6,538
3 жыл бұрынVideo used for teaching on module 400484 Cells and Organelles at the University of Hull
@Mfdwoi 2 жыл бұрын
Most helpful answer I've found online, thank you!
@ImAli01 2 жыл бұрын
This was great helped A LOT Thanks!
@user-mj8vw1bd2o Жыл бұрын
Thanks
@haticeboke6584 Жыл бұрын
Thank you so much. I have been searching this information for more than 1 week. You explained it in an understandable way. Can we use this calculation for NADPH?
@katharinehubbard5043 Жыл бұрын
Yes - NADPH absorbs light directly at 340nm so you can use Beer's law directly. Note that NADH also absorbs at this wavelength, so you will need to design your experiment carefully to ensure it is NADPH you are measuring specifically. Hope that helps!
@balakishore1274 Жыл бұрын
If the reactant and product absorb same wavelength then it is possible to use spectrophotometer to find the absorbance
@katharinehubbard5043 Жыл бұрын
No - in this case if there is a 1:1 conversion of reactant -> product and both absorb at the same wavelength you won't be able to measure the change in absorbance. You'd need to extract the product specfically
@anjalinegi6204 11 ай бұрын
I want to know the molar absorption coefficient of phenylhydrazone. Where can i find. Because instead of NADH, i have glyoxylate phenylhydrazone formation as product
@katharinehubbard5043 11 ай бұрын
I don’t know as have never worked on that reaction but looks like this paper might be useful? pubmed.ncbi.nlm.nih.gov/10731695/
Medicosis Perfectionalis
Рет қаралды 81 М.
Biology Lectures
Рет қаралды 94 М.