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Hey Friends,
cDNA (complementary DNA) is synthesized from an RNA template. This is done by Reverse Transcription. In this example, the oligo d(T)-primers are used to prime the cDNA synthesis. An alternative would be to use random hexamers as primers. cDNA is synthesized before qPCR to check gene expression levels.
qPCR:
kzfaq.info/get/bejne/n9tkppZ5x8nDkqs.html
References:
Recommended: www.nature.com/articles/nmeth0205-151
More:
geneticeducation.co.in/reverse-transcription-pcr-principle-procedure-applications-advantages-and-disadvantages/
www.ncbi.nlm.nih.gov/pmc/articles/PMC338195/
Cheers
Henrik