Such a cliff hanger too, did he get to pack her column at the end?
@dianeallen2328 жыл бұрын
Really well done. Much better than any of the corporate KZfaqs on the subject with a nice reference to the foundational publication on Flash.
@scatterbrainart7 жыл бұрын
5:25 Sick burn!
@davidhenry51242 ай бұрын
Jesus XD
@Xalexalex12 жыл бұрын
Labs *this* clean only exist in movies =D
@IamJiva4 жыл бұрын
never partake crude
@astropgn10 жыл бұрын
I liked how you cited a real scientific article and not just a blog's post, like every other tutorial on the internet :P, thanks
@kristyelbel255511 жыл бұрын
There is a good paper by W. Clark Still in JOC (Vol 43, No 14, 1978, pg 2923) which shows a table of typical fraction sizes with different size columns, volume of eluant, and sample loading size.
@ChemUCSD12 жыл бұрын
Viscous oil is treated similarly to solids. Use “wet loading” if it is soluble in a solvent that is of equal polarity to your initial running condition. Use “dry loading” if it is soluble in a solvent that is more polar.
@kristyelbel255511 жыл бұрын
In our lab, we usually stick to fractions about 1/10th of the column volume. For example, if you use 25g of silica gel you should collect fractions of about 3 mL. This can be adjusted depending on your separation as well. Larger fractions can be taken for very good separations and smaller fractions for poor separations. This can help limit mixed fractions.
@Georgiaboy_8 жыл бұрын
Wow this is a great video! I'll probably have to watch it a few times to keep up with everything however!
@saisadhareddy54885 ай бұрын
Thanks to all the team members for providing the wonderful content I hope you will make many more videos which helps many students, research scholars & others to get to know the basic understanding of such techniques & methods
@josephmeredith19327 жыл бұрын
This video is incredible. Excellent work!
@jaykemm3472 Жыл бұрын
And later, Nathan gave Kristy a column of his own.
@hkkhgffh36135 ай бұрын
You are so disgusting!
@anees112224 жыл бұрын
The best thing in this video is how much Silca is needed . You guys did a fabulous job👌
@kcore96885 жыл бұрын
Best flash column tutorial on youtube that Ive seen
@xavierlinn50138 жыл бұрын
Great video. Cheers from UC Berk.
@shireen34922 жыл бұрын
Awesome video! My mentor suggested I watch it and it was super helpful!!!!
@Mikelmangold7 жыл бұрын
I'm doing it tomorrow in the lab. Thanks from Freiburg, Germany :)
@saltypablo11 жыл бұрын
Nice Free advertisement fro Chemglass.
@samanthabyrne297011 жыл бұрын
This was excellent,thanks guys.
@soumitdutta80492 жыл бұрын
Very Beautiful Demo and Explanation!
@daivdormaza19879 жыл бұрын
This helped me review a lot to get started with my research thanks!
@XiangxiMeng7 жыл бұрын
Educative yet entertaining...
@youcefwounds49665 жыл бұрын
Great Video, Thank you so much💖💖💖💖 I will use the dry loading next time, unfortunately, I did not know about it before.
@IamJiva4 жыл бұрын
a piece of filter paper covering the porous glass filter plate, can facilitate glass washing, or even make it feasible and glass-lossless maybe... sometime f.e. the precipitate from the quenching of LAH RM, that sometimes unfiltered through cotton wool(for example), fortunately does not block filter paper, allowing filtering. my favorite chose with THF+LAH RM - to quench with a minimum of plain water(only H2O) - until whitening - adding not faster than 0.5 ml of the next portion of water every 30 minutes. Then white suspension diluted with an equal volume of DCM, shake and leave to settle for 30 minutes-to form compact(only 5ml from 1gm of LAH) crumbly solids, that can be filtered by gravity through cheesecloth... results flowing slow only with some rare waxy-nature products(but not with bad weather influence), that also accompanied with same difficulties in tradition LAH workup procedure, but even in that slow-workup synthesis - you will not need a three-liter Schott funnel instead of a one-liter cloth filter in pressurized-filtering-jar
@johnmcg58657 ай бұрын
5:23 Jesus.. Christie is sassy...
@aggumbi9 жыл бұрын
Awesome video! super informative and very digestable. Also, I really dig the music. Any idea what the name of the track is? The song name isn't cited in the credits; only the composer is.
@raspudding72204 жыл бұрын
Oblivion dialogues in the Arcane University, Department of Alchemy
@zainabdookhy24807 жыл бұрын
why isn't she blinking the the first two minutes
@shaikhfirdousmuskanR7 жыл бұрын
Awesome video Thank you people
@jennihaataja9327 жыл бұрын
Kristy looks like she is having super hard time with her teeth.
@user-vw5xi2sq9k3 жыл бұрын
All is well, except for TLC in a cup. There must be a Camera for TLC, saturated with eluent pairs.
@somebassdude10 жыл бұрын
Thanks! This helps a lot :)
@Pastelpanda7966 ай бұрын
4:43 wait what, everytime i do that, the silica is stick into the flask
@salehchem4376 Жыл бұрын
happy new year and Thank you so much for this video. Is there standard to use silica gel in column, I mean if my sample a little bit like (0.01 gram), how much of silica gel use here? in contrast, if I have a lot of samples such as (1 gram or more), here, how much of silica gel have to use? I mean is there ratio from silica gel to sample to use in column, please tell me
@locoporCristo9765 жыл бұрын
Amazing video! I would like to read the paper that it´s mention in the video, could you drop it here please.
@filipecardozo2 жыл бұрын
great video
@MaryFernandez13 жыл бұрын
Wow, another awesome video, Dr. Weizman. You know how to make learning Chemistry fun!
@marcelalopez3113 жыл бұрын
Conocí a colegas que purificaban en una bureta y corrían al Carls Jr por que están plenamente convencidos de que el sueño americano es posible para ellos.
@Snowpatrol9117 жыл бұрын
Thanks from U of Montreal!
@salehchem4376 Жыл бұрын
thank you. If I used wet packing of silica gel to column (slurry), here, can I use any solvent for silica gel until reached slurried, or same mobile phase or must use only hexane.
@GreenOrgyKing3 жыл бұрын
So would this be considered 'Art Haus' or more 'Classic Cult"? Also, I didn't go to school for serious Chem, do people really think running Flash Chromatography more slowly gives better separation? *SMH* I guess they're confusing it with crystallization?
@ChisomOkolie128 ай бұрын
So cool👏🏽👏🏽👏🏽
@pelegsap8 жыл бұрын
Wait, Dr. Haim Wiezman, a chemist... is he planning to become the President of Israel one day? :-D
@IamJiva4 жыл бұрын
Allway - chemistry rules at least the atomic world, I want president David Nutt (h t t p s : / / w w w.) kzfaq.info/get/bejne/aLWTkqp3m5vSkps.html be protonic - be smart! :-))) even carbolic acids better than "neutral hydroxyl in society"
@IamJiva4 жыл бұрын
consuming a synthetic product is better than intake of unificated killing, you can even get rid of oxidation process - by reducing vinegar or acetaldehyde, but egregor's bad reputation defiles even something just marked by some individual sign of degradation
@chemistry75545 жыл бұрын
With experiment both chemistry wahooooooo............
@hkkhgffh36135 ай бұрын
I would like that she takes care of my column.
@DatsuJSB2 жыл бұрын
4:54 no that's not a solution, it's a heterogeneous mixture.
how can you load very viscous oils? -is it the same technique as drying? -by the way great video
@salehchem4376 Жыл бұрын
If I have solid sample containing components, can I use any solvent to dissolve it? Or I have to use only the same mobile phase as solvent to dissolved sample? Another case, if must use same mobile phase, but my sample can not dissolve, here, please I need solution to this problem. can I use any solvent? thank you alot in advance
@sheldonyy141412 жыл бұрын
good job, thanks
@victorscrt10 жыл бұрын
Where in the world would i buy the powder for the silica gel or search on ebay or amazon to get it?!?! Please help me, and would any university carry it at their chemistry stock room? Thanks so much for the vid!
i am workin on column shromatography.. can you help me in guiding that is there any way of setting some manual punp system to my column that will convert it from standard column chromatography to flash chromatography...
@honeybunbadger11 жыл бұрын
What is the correct volume you should collect for each fraction? How does it depend on the amount of silica or crude?
@rentaros647520 күн бұрын
Do I add the sand also after solid loading?
@ahmadt20403 жыл бұрын
Great🌺👍
@alhaeri13 жыл бұрын
thanks
@cmorera11 жыл бұрын
epic dialogue
@Blederama11 жыл бұрын
it's not a ''pie pet'' but a pipette
@juanra79452 жыл бұрын
Amé
@bragasgambit2 жыл бұрын
No blood? This movie s not written and directed by Quentin Tarantino tho. Even though its an excelent one! lol
@jhyland875 жыл бұрын
Why is it called "flash" chromotography?
@chuckichas5 жыл бұрын
You use pressure to force the solvent mixture through the glass as opposed to relying on gravity alone to perform the run which would take a substantial amount of time in some cases
@locoporCristo9765 жыл бұрын
Amazing video! I would like to read the paper that it´s mention in the video, could you drop it here please.
@BeepingSheep5 жыл бұрын
Maybe if you read it yourself, you wouldn't need a link. Ever think of that?