ChUG Cytometry Presents: Introduction to Spectral Unmixing

  Рет қаралды 5,892

ChUG Cytometry

ChUG Cytometry

Күн бұрын

Thank you for joining us for this ChUG-Cytometry webinar. Please join the ChUG forum (www.chugcytometry.com/forum) to give us your opinion on this presentation or suggestions on future topics you would like to see discussed.
Spectral Flow Cytometry can be a tricky technology: while acquisition can be fairly easy on most platforms, a good understanding of the mathematics behind unmixing is required to generate quality data.
We are very excited to host Dave Novo, President and Founder of De Novo Software (FCS Express). There is no one better to guide us through these new concepts and help us apply them in order to improve our science.
David received his PhD in Biophysics from UCLA. His research concentrated on developing novel optical and mathematical techniques for measurements and analysis of localized fluorescent Calcium transients from a microdomain within a muscle cell. David also built several confocal microscopes, as well as writing the data acquisition and data analysis systems. Prior to commencing his PhD, David worked with Dr. Howard Shapiro, one of the leaders in the field of flow cytometry, performing research on membrane potential determination in bacteria using flow cytometry. During the course of this research they built several flow cytometers custom made for bacterial measurements. Two papers were published on the subject. David still maintains close ties to the flow cytometry community through his appointment to the Data Standards Committee of the International Society of Analytical Cytometry. This committee maintains the flow cytometry data file standard and deals with issues standardizing all sorts of flow cytometry related data storage and analysis issues. David frequently consults on data analysis issues related to flow cytometry and collaborates on many projects involving novel data analysis requirements.
The discussion will be moderated by:
- Ryan Duggan, Senior Scientist at Abbvie
- Laura Johnston, Associate Scientific Director at the CAT Facility at the University of Chicago
A Q&A period will follow. A recording of the conversation will be made available on the ChUG website.

Пікірлер: 8
@strongulocentrotus
@strongulocentrotus Ай бұрын
The guy deserves a lot of respect for a clear and simple explanation of complex things. Great job!
@debajitbhowmick7079
@debajitbhowmick7079 3 жыл бұрын
An excellent source to clear confusion...
@charlieintampa6769
@charlieintampa6769 2 жыл бұрын
Super happy that I found ChUG videos! After going through endless hours of flow material on the internet, where in an hour at best there is one sentence that is actual news to me, your videos are full of things that I always wanted to know, or at least things that are confirming what I thought.
@chugcytometry3284
@chugcytometry3284 2 жыл бұрын
Hi @Charlie inTampa! Thanks for the kind words! I'm with you here, I think there's a lot of flow stuff that gets repeated over and over. It's nice to have a place where we can do something else!
@raymondlannigan7607
@raymondlannigan7607 4 жыл бұрын
Easy to understand matrix algebra presentation. Thanks!
@dwgalbraith
@dwgalbraith 4 жыл бұрын
Very nice presentation! Congratulations.
@hemantagrawal3772
@hemantagrawal3772 4 жыл бұрын
Well done Dave
@brettransegnola1095
@brettransegnola1095 2 жыл бұрын
Hello! Great video! Just a comment but the (fitc pe) matrix should be vertical to be correctly representing the matrix multiplication
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