This is a type of enzyme inhibition where the inhibitor binds the active site of the enzyme. As a result of this binding the Km value changes while the Vmax remains the same.
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@faruqsosanya4550 Жыл бұрын
Great video .thanks this this wonderful piece. 5:05-5:15 doesn't seem mathematically right though But it's a great video. Watched one of you video on inhibition graph it was very helpful. Kindly check this video at that point. A/(b+c) is not equal to a/b +b/c
@dodo-zj8rw9 ай бұрын
Then what
@ryanwalsh4463 ай бұрын
You are right the math is wrong here but only because the video is poorly explaining what should be happening To do a LWB plot the equation needs to be inverted so you now have 1/Vo = (Km(1+I/Ki)+[S]) / Vmax[S] Which can then be divided into 1/Vo = (Km(1+I/Ki)/ Vmax[S] + [S]/ Vmax[S] and then reduced to 1/Vo = (Km(1+I/Ki)/ Vmax[S] + 1/ Vmax So by failing to invert the equation at the beginning the video was incorrect and like you said A/(b+c) is not equal to a/b +b/c It should have been Invert A/(b+c) then (b+c)/A = b/A + c/A But this is a moot point when the whole competitive inhibition equation is fundamentally wrong as a chemical equilibrium for inhibitor binding can not provide any information on the effect of inhibition. 1+]I]ki = 1/(1-([I]/[I]+Ki)) So not only is this equation defining inhibition with a binding curve that says nothing about magnitude of inhibition its using an inverted binding curve that is quantifying the fraction of the enzyme population not bound by the inhibitor. Check out my video if you want to learn more kzfaq.info/get/bejne/ns2HrJOBrtzPo6s.html
@richardshane4564 жыл бұрын
No comment, sounds like you're extrapolating data with minimal reference to what is happening at the particle and molecular functions
@ryanwalsh4463 ай бұрын
That is exactly what is going on inhibitory theory is biased derivation that doesn't recognize the difference between a chemical equilibrium and the effect the inhibitors binding has on the enzyme. Enzyme Inhibition's fundamental flaw kzfaq.info/get/bejne/Y62jdcVny7nMmX0.html
@ihuomaotika26562 жыл бұрын
Thank you!
@mumtazahmad26834 жыл бұрын
Great
@allisonbasye11973 жыл бұрын
Does not make any sense whatsoever, especially with ZERO help on plotting in Excel!
@jyothigorijavolu85753 жыл бұрын
Here,what is mean by Kcat sir??
@hawkseye30352 жыл бұрын
Catalyst
@medicallifewithjohn83433 жыл бұрын
Awesome
@tukurummulkhairi97943 жыл бұрын
Thank you so much
@hajaraboulaola49132 жыл бұрын
Thanks
@mohamedsahab49926 ай бұрын
that is useless
@umaelumalai4523 Жыл бұрын
M1m
@richardshane4564 жыл бұрын
With reference to time what is the metric to express your E ES EI expression relative to a time metric? From these differing states? Maybe I missed something,... also will enzymatic oppression occur over all biological entities regardless of their overall state or will local dietary and chemical exposure, will it alter the enzymatic expression using time reference as the metric?
@ryanwalsh4463 ай бұрын
complicated question but yes expression changes with time and environment but unfortunately classic inhibition equations are not meant for time course analysis and only approximate the initial equilibrium established when the enzyme, substrate and inhibitor first come together.