Connecting Living Neurons to a Computer

Connecting Living Neurons to a Computer | Part 2

Рет қаралды 705,078

Ай бұрын

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More resources:
www.jove.com/t/2056/how-to-cu...
potterlab.gatech.edu/publicat...
journals.plos.org/ploscompbio...
www.nature.com/articles/s4192...
engineering.cmu.edu/news-even...
www.scholarpedia.org/article/S...
• Spiking Neural Network...
• The Cortical Column
_______________________________________________________________________
Previous Episode: • Growing Living Rat Neu...
First Try: • Growing Human Neurons ...
Meat Robot: • A Leaf Made of... Meat??
Meatcubator: • This Machine Grows Liv...
Meat in Gatorade: • Turning Gatorade Into ...
Plasma Metal Coating: • Metal Coating using PL...

Пікірлер: 2 800

@thethoughtemporium Ай бұрын

If you'd like to support our work and get yourself an array poster, check out our store: thethoughtemporium.ca/products/uv-glow-poster

@teemteem Ай бұрын

maybe

@Alceste_ Ай бұрын

Did I get "printed locally here in Quebec" right? :o

@noi0124 Ай бұрын

@@Alceste_ yes, the link to the screen print studio is in the infobox

@Chitose_ Ай бұрын

*THE PART TWO WE HAVE ALL BEEN WAITING FOR* 🎉🎉🎉🎉🎉

@murch5054 Ай бұрын

Hi, i think the link isn't working

@Durei.rei_ Ай бұрын

Imagine being conscious just to find out your purpose is to do a playthrough of doom

@kirralabas5315 Ай бұрын

Somehow that doesn't seem that bad, it's a decent life purpose

@AllanSchon Ай бұрын

That's more or less how I remember my high school years...

@colematlock7755 Ай бұрын

A fulfilling life

@dercncplaner Ай бұрын

you know that there are theories that we live in a game. Soooo

@JohnDlugosz Ай бұрын

That's a lot better of a purpose than to pass the butter.

@nl4006 Ай бұрын

The reason the neurons all died after the initial seeding is because you didn't rinse the PEI off properly and/or didn't dry it properly. While PEI is the best pre-coating agent in the business, it is also an extremely potent membrane perforant when in solution, so any miniscule picogram of the stuff you left unpolymerized is gonna be jumping from cell to cell, shredding their membranes. Rinse at least five times with maximal volume your growth vessel can take, and dry the vessel overnight in the LAF bench, otherwise you're just wasting expensive reagents. Source: neuro lab-slave for 10+ years

@kevincuevas8877 Ай бұрын

Bump and like for visibility

@sinedddmk8996 Ай бұрын

Sounds like you are an ideal buddy for this dude to keep track of details, if you didnt have a job already.

@lucasmeneses8341 Ай бұрын

Up!

@esra_erimez Ай бұрын

I was about to say that exact some thing. However, you left out the part about the goop in the gloopinator. You really need to check that too.

@user-nb6mv3ny8i Ай бұрын

post doc in a university?

@jackhazardous4008 Ай бұрын

>wake up >your entire existence is DOOM >pass on thinking that was all there ever was to life

@ghoulbuster1 Ай бұрын

Same as it ever was.

@poupeuu Ай бұрын

>wake up >your existence is this one >pass on thinking that was all there was to life

@jeremymcadam7400 Ай бұрын

@@poupeuu This is why I choose to participate in 5 dimensions

@gondolaone7748 Ай бұрын

@@jeremymcadam7400 rookie numbers, all of us in the 6th dimension are looking down on your struggles, purely for amusement I might add

@j0rtsy 29 күн бұрын

Plato’s “The Cave” is Doom.

@martyshrekster Ай бұрын

"Yeah I'm doing research on neural networks." "Oh, what algorithms are you working with?" "Rat"

@legenberry3217 Ай бұрын

Underrated 😂😂

@denifnaf5874 Ай бұрын

Can it run doom?

@Beeti1 Ай бұрын

That's the medium, not the algorithm.

@creationstation6035 23 күн бұрын

Rotational Additional Tactics RAT 🐀🐀🐀 🐀. 🐀. 🐀. 🐀🐀🐀🐀🐀 🐀🐀🐀. 🐀🐀. 🐀 🐀. 🐀. 🐀🐀🐀. 🐀 🐀. 🐀. 🐀. 🐀. 🐀

@John-wd5cb 22 күн бұрын

and where do you work? A weapons corporation..

@hhhpestock951 Ай бұрын

"We're naming it: The Torment Nexus, after the hit book, Don't Build The Torment Nexus."

@spencerharrison9680 Ай бұрын

Bro is literally a classic evil scientist at this point

@FateStayN1ght Ай бұрын

I cant stop laighting at that !

@harriehausenman8623 Ай бұрын

@@rubikscuber9277 Absolutely! (in the classical meaning of the word, like in "The Gay Falcon" (1941)) 😄

@rubikscuber9277 Ай бұрын

@@harriehausenman8623 sorry I mean to say Yay

@Otzkar Ай бұрын

@@spencerharrison9680 *tech company

@hakajiru264 Ай бұрын

Painstakingly growing a brain slice only to pepperspray it moments later -.-

@ayybe7894 Ай бұрын

Such is the cruel nature of life

@LordDragox412 Ай бұрын

Do not build the Torment Nexus. But if you do, do not torment the Torment Nexus with cayenne pepper flakes.

@Imtoolazytodothis Ай бұрын

Sounds like a middle-age crisis

@harriehausenman8623 Ай бұрын

Almost human

@Asmodis4 Ай бұрын

every time i found one of his videos i get this harlan ellison vipes and i dont know why.

@X-SPONGED Ай бұрын

3:20 "You merely adopted the sports drink. I was BORN in it, MOLDED by it"

@Prucake Ай бұрын

"Don't build the torment nexus" YOU HAD ONE INSTRUCTION.

@omn1cr0ngaming66 15 күн бұрын

Instructions unclear, built torment nexus

@kayjersch4959 Ай бұрын

3:50 "long term, i want to simulate a whole brain" fucking WHAT

@thewizard1 Ай бұрын

Bro is a mad scientist 😭

@harriehausenman8623 Ай бұрын

Naturally. 🧐

@samuelhomberg9075 Ай бұрын

I think he's not talking about a human brain (so far...).

@CarlosSpicyWang Ай бұрын

@@samuelhomberg9075You want skynet? That’s how you get skynet.

@wesleymays1931 Ай бұрын

@@samuelhomberg9075give him time...

@aerofight5994 Ай бұрын

Strange man in a sweaty lab coat staring at a rat in the corner of the room. Thinking to himself ... "Can it run DOOM?"

@ussuratoncachi Ай бұрын

More correctly: "Can it play DOOM?"

@mishtrong Ай бұрын

@@ussuratoncachiCan it RUN doom? Can I use its brain as a computer to run doom?

@Spartan11117777 25 күн бұрын

Can it run in a Hamster Wheel to power the Platform running Doom?

@joshcarriker6958 24 күн бұрын

The body is just the case for the computer

@TheGrammarNazi123 23 күн бұрын

"👍✌☠ ✋❄ ☼🕆☠ 👎⚐⚐💣✍"

@SophieYui Ай бұрын

Imagine being the neurons "okay, so we can't sense anything, and we probably can't do anything, but we AAH SPICY!"

@thecoolestofguys Ай бұрын

the torment nexus will be built!

@harrisonlichtenberg3162 Ай бұрын

Homie really dumped hot chem on raw nerves just to see the funny lights

@rubyred186 Ай бұрын

I have no mouth and I must scream.

@matturner6890 Ай бұрын

you're all replying to a bot, pay attention.

@Dann.y Ай бұрын

@@matturner6890WE ALL KNOW! AND DID YOU KNOW THAT YOU ARE REPLYING TO HIM TOO?!

@isodoublet Ай бұрын

Don't you just hate it when you're chilling in the torment nexus when all of a sudden your entire being is overcome with an extremely powerful chemical irritant?

@orsonzedd Ай бұрын

I'm glad someone's finally getting around to building the torment Nexus

@ayybe7894 Ай бұрын

Well it wasn't going to build itself.

@Imtoolazytodothis Ай бұрын

@@ayybe7894 your not wrong lmao

@LordDragox412 Ай бұрын

Me: Wait, where does the "torment" come from in Torment Nexus? The Thought Emporium: Cayenne pepper flakes. Look at the neurons screaming in torment. Delightful. Me: Oh yeah, that makes total sense, thanks.

@jakobweisser8044 Ай бұрын

I'm just glad it's the humble mad scientist and not the techbros.

@warmitchine3388 Ай бұрын

@@jakobweisser8044 "We built a neural interface on an unregistered cruise liner off the coast of Panama that mines cryptocurrency. We just need your donation of $200,000 to keep it running and to pay the illegal waste dumping fees." -techbro neuroscientists

@anonymousapproximation8549 Ай бұрын

"It's designed for researchers, not a bunch of crazy nerds" "Corporate needs you to spot the difference between these images"

@mihael64 Ай бұрын

Researchers aren't nerds, they're just crazy

@ttty2242 Ай бұрын

budget

@SioxerNikita Ай бұрын

@@mihael64 But... they are also usually nerds XD

@vidal9747 Ай бұрын

@@mihael64 My advisor literally can not physically stop himself from talking about Dune if he drank more than 2 beers... And not every researcher is crazy. Some can even manage work life balance. Not anyone I know, but I heard some can!

@blakksheep736 Ай бұрын

The only difference between a researcher and a nerd is that researchers are paid.

@BionicleFreek99 Ай бұрын

Finally! Man-made horrors WITHIN my comprehension!

@cefcephatus Ай бұрын

9:29 "Why those ingredients? I have NO IDEA!" Yep! That's how you know someone is doing real science and not just teaching you prepared lesson.

@johnpooky84 18 күн бұрын

(*Cave Johnson enters the chat*)

@aimalisapro123 10 сағат бұрын

@@johnpooky84PORTAL MENTION

@nuclearocean Ай бұрын

Can't wait till I have to feed my computer and overclock it with some spicy red pepper

@henrysanecdotes5323 Ай бұрын

*losing in valorant* MY FRAMES! GET THE PEPPER

@AngryAlfonse Ай бұрын

"Hey guys, my GPU is struggling to hit 120fps. Gotta go toss a 5 hour energy into the growth medium circulator."

@alephnole7009 Ай бұрын

Sprinkling it in like fish food into a brain in a tank

@harriehausenman8623 Ай бұрын

*playing fps game* *loosing connection* "Guys, my machine just had a seizure."

@navinvent Ай бұрын

What i learnt is that injecting pepper into the brain will be an effective form of torture.

@SophieYui Ай бұрын

"Slightly sentient enchilada" caught me off guard xDDDD

@steamerpowered Ай бұрын

Yup, not a sentence I expected to hear this morning... lol

@nullpoint3346 Ай бұрын

It's only sentient, we're fine _(for now)._

@matturner6890 Ай бұрын

you're all replying to a bot, pay more attention.

@nullpoint3346 Ай бұрын

@@matturner6890 Do you think we care?

@oompalumpus699 2 күн бұрын

🤖🍑

@christophepetry6782 Ай бұрын

I'm a doctoral researcher and Neuroscientist. When it comes to culturing medium for neurons morphology can tell you a lot about the state a cell is in (this goes for most cell types). To me the classic choice of Neurobasal gives you cells with shrunk cell bodies (the nucleus is tightly shrink wrapped if you will) and long complex truncated neurites (dendrites and axons). This tells you that these cells are "mature" neurons. They are not quite mature because you literally need let time pass for them to become mature, interestingly the time of maturation depends on species and is much longer for human neurons compared to mouse neurons.

@TheAmazingAnimator 23 күн бұрын

I think that's because rats have less neurons to mature overrall. also because other animals (I think) gain consciousness faster

@christophepetry6782 23 күн бұрын

@@TheAmazingAnimator it is more about complexity of their connections and morphology and less about the overall number of cells. In culture you can observe the same time discrepancies between neurons of different species, so it is an inherent quality of the cells. We are not clear on what level of consciousness other animals have , since we can’t test for it. You probably mean animals become independent much earlier, since humans literally complete their development outside the mothers body. During evolution our heads got to big for our pelvises so we have to be born “prematurely”.

@Rosencrantz17 Ай бұрын

"What is my purpose?" "You play DOOM." "RIP AND TEAR! RIP AND TEAR! RIP AND TEAR!"

@Hexawatt 29 күн бұрын

Rip And Tear, or R.A.T. for short.

@Cacowninja 25 күн бұрын

Until it's done!

@pizzapie5356 19 күн бұрын

Man is gonna build a murder hungry superintelligence

@professorstevepotter Ай бұрын

9:55 Aha! apropos to my previous comment about cell density, I see how to solve your problems: We always added a small droplet (50-100ul) of cell suspension added to an EMPTY dish, with just a 3mm laminin wet spot. Calculate what density you need in the suspension to get 50k-100k cells in that droplet. After the cells have settled and adhered (30 min?) carefully add the rest of the medium to flood the dish. Now you will have very dense cultures and signals on every electrode.

@MyDadSaid Ай бұрын

Thank you. We really appreciate all your input.

@AcceptableAsGenerallyDecent Ай бұрын

dr stone would probably accomplish this with the power of jump cuts and eye closeups

@noi0124 Ай бұрын

Power of anime

@guarana-suquinho 23 күн бұрын

And that's why he is called Dr Stoned.

@johnpooky84 18 күн бұрын

I loved that show! Hoping for a Season 3.

@Numbers-gStands 12 күн бұрын

@@johnpooky84 I was wondering the same thing and I think there is a season 3 but it’s not on netflix yet?

@johnpooky84 12 күн бұрын

@@Numbers-gStands Dr. Stone's on Netflix? I watched it on Crunchyroll.

@TornaitSuperBird Ай бұрын

10:09 "Hey how are the neurons growing?" "They all died" *AD* ...had a hard time trying not to chuckle in the doctor's office.

@WurliMonkhaven Ай бұрын

Hope the appointment went well! God bless!

@aeea3306 3 күн бұрын

What

@davef21370 Ай бұрын

It's brilliant but a little sad to think that a dish full of rat neurons could potentially beat me at Doom.

@breadboi3837 Ай бұрын

the ultimate cheat system, full automation cheats; I could grind war thunder off a chunk of rat brain

@zachrowe6271 Ай бұрын

@@breadboi3837imagine getting banned for rat brain botting 😂

@harriehausenman8623 Ай бұрын

@@zachrowe6271 🤗

@user-burner Ай бұрын

To be fair, you also have to think about, yknow, having a body and pressing keys and shit.

@NiclasOvik Ай бұрын

@@user-burnerTrue that, all about focus, reaching flow state.

@professorstevepotter Ай бұрын

My other suggestions seem to have been deleted, perhaps because I linked a paper of ours. I guessed that you may be killing cells with too much light. Phototoxicity is a huge problem with any fluorescence microscopy. To reduce it, use phase contrast (not fluorescence) microscopy and use longer-wavelength (red) light. Also, when doing fluorescence imaging, use a lower-NA lens, more sensitive cameras, dimmer illumination, longer wavelength excitation, and shorter exposure to light.

@thethoughtemporium Ай бұрын

Feel free to send me an email with all of the suggestions so they don't get lost! And they're greatly appreciated as always. We only use phase contrast when checking on the cells. Fluorescence was only used at the end for the sake of the experiment, and the dye was only used on one of the arrays after we had finished all other experiments. The arrays are also only ever left on the microscope for as brief a moment as possible, but the phase contrast images aren't as pretty so I don't use that footage in the video as much. I also saw you recommend concentrating the neurons in the middle. I read that in the instructions and that's how we attempted it the first time when the cells died. We went with adding the neurons after the media because we knew for sure the neurons would survive as we've tested that method a lot and couldn't risk another culture dying. We were pressed for time to get the video out. And for our arrays the electrodes are REALLY spread out, so we're gonna need a high neuron density across most of the surface of the array anyway. You'll notice I made the walls of our arrays taller than the multichannel ones. It was specifically because I wanted to increase the amount of growth media per well so the neurons don't deplete it as quickly and to account for an eventual much higher neuron density across the array. We have wanted to upgrade our camera on the microscope for a while, but the scope itself was a HUGE upgrade. It's new for this video and it cost a pretty penny. But it's built exactly for this kind of experiment so we can switch out the camera next. I also want to move to a red dye to lower the illumination wavelength as you say. And also just to make the videos look more interesting than always green. We ran out of time, but we also got a tubulin specific dye we're gonna show off next time that would look best against an otherwise red stained neuron. And we picked up NucBlue so we can stain the nucleus properly. One of the shots of the neurons firing included it from the first well we tested it on. I overlaid it. It's subtle but makes the neurons look nicer and the nucleus visible.

@thethoughtemporium Ай бұрын

Also, I've set your account to always show comments on the channel now, so they shouldn't get deleted.

@cougar9610 16 күн бұрын

Imagine having a CPU that's literally just a brain, how fast would it compute compared to a normal cpu?

@RevolutionPony Ай бұрын

I loved reading "Don't Build the Torment Nexus!" When I was a kid. I don't remember what the moral of the story was, but I really liked the lore, aesthetics, and world building. I'm so glad someone is bringing my favourite sci-fi to life! We really do live in the future!

@Penguinman2.0 Ай бұрын

Wait I can’t tell if this is genuine or not

@DawnPetrichor1 Ай бұрын

@@Penguinman2.0Neither can I… On a second reading, I don’t think it’s genuine, “I don’t remember what the moral of the story was” seems like it wouldn’t be there if it was.

@SissypheanCatboy Ай бұрын

@@Penguinman2.0 The book doesn't exist, its from a meme talking about how techbros proudly claim inspiration from scifi stories where the whole moral is NOT to build the technology featured in the movie. Like how vr/metaverse nerds constantly say "its like the Matrix in real life"

@adora_was_taken Ай бұрын

@@SissypheanCatboy or more recently, openai comparing gpt-4o to the movie "her"

@vipture. Ай бұрын

@@SissypheanCatboyThe first thing we did after GPT-1 showed promise was hook up the next version to the internet to learn from. It's like the people at the forefront of advancement have turned dystopian prophecies into a checklist..

@Mechthorian Ай бұрын

24:40 Surely this is the worst O-Ring disaster in the scientific profession...

@noi0124 Ай бұрын

New challenger appeared!

@AresHoax_9 Ай бұрын

@@noi0124it’s the challenger space shuttle ! Ready? Set Fight!

@harriehausenman8623 Ай бұрын

Maybe they just have to chill… *checking notes* On no, wait. That was NOT the cure 😆

@JinKee Ай бұрын

Too soon

@m4rcyonstation93 Ай бұрын

Context? (Curious)

@thingyjim Ай бұрын

Hey! I’m messing with neurons at the moment in my PhD and I saw the exact same morphology in DMEM/F12, I was stumped what was causing it! As a precaution we’re genotyping with ICC and qPCR since we’re deriving from iPSCs - anyway, love the video! This project is so cool!

@Isteak80 Ай бұрын

"I want to build up to simulating a whole brain" followed by "...so anyway" caused me at least some level of concern.

@hakajiru264 Ай бұрын

Neuron: Help I'm peeling Neurobiologist: SUGAR FREE HORSE JUICE

@darealsherlock8026 Ай бұрын

DOCTOR STEVE POTTER CONTACTED YOU! THE DOCTOR POTTER! This is surely indicative of success to come

@akatsukilevi Ай бұрын

For the ones randomly watching this and has no idea who Steve Potter is and why it is a big deal It is as if Jesus Christ himself showed up for a theology class

@Imtoolazytodothis Ай бұрын

A truly impressive feat!

@harriehausenman8623 Ай бұрын

What a Legend.

@jimcobbler3954 Ай бұрын

I’m sure he’s never heard this joke, but he sounds like a wizard in this field.

@TekedixXx Ай бұрын

@@akatsukilevi I can't not stop thinking about Professor brothers now lol

@katofmine Ай бұрын

18:09 “slightly sentient enchilada” made me inhale half my beer

@gevelegian Ай бұрын

If youtube was just this type of content but with every expertise/experiment currently going on. It would be just perfect harmony.

@Imtoolazytodothis Ай бұрын

The perfect combination of long-form content, diligent and passionate work, and humor!

@LordDragox412 Ай бұрын

Meanwhile KZfaq: Demonetized.

@yaysuu 16 күн бұрын

TestTube

@gevelegian 15 күн бұрын

@@yaysuu holy smokes you're onto something!

@Gidon147 8 күн бұрын

not only that, it would inspire young people from age 2 onwards to become youtubers and make the next big scientific discovery/invention!

@titusbaum9690 Ай бұрын

Neuron-piloted flesh mechs growing neurons, simulating neurons, looking at neurons, visualizing neurons. Ah yes, Neuron Activation.

@audriusskebas3099 Ай бұрын

Soon we shall have a flesh automaton animated by neurotransmitters!

@Gakulon Ай бұрын

Do not dare to insult the purity of steel by comparing mechs to abominable flesh

@Mate_Antal_Zoltan Ай бұрын

Bro just called God's greatest creation "neuron-piloted flesh mechs"

@audriusskebas3099 Ай бұрын

@@Mate_Antal_Zoltan It's a Cruelty Squad reference xP

@Mate_Antal_Zoltan Ай бұрын

@@audriusskebas3099 guy who has only played Cruelty Squad in his entire life: "I'm getting real Cruelty Squad vibes from this..."

@thesuperfluousone2537 21 күн бұрын

"Don't build the torment nexus." "What'd he say?" "I don't know. I think he said build the torment nexus."

@erezdude123 Ай бұрын

You are one of the main reasons I am perusing my masters in synthetic biology!!!! I love your videos man hope you keep doing what you do :)

@jvdos Ай бұрын

THE DAY HAS COME BROTHERS! 🗣️🔥🔥

@Log4Jake Ай бұрын

FINNNNALLLLLYYYYY

@n0o0b090lv Ай бұрын

LETS GOOOOOOO💯💯

@Xxrasierklinge7 Ай бұрын

Ikr! I've been waiting for this for what seems like FOREVER!!!!!

@lopatoj Ай бұрын

yay

@quentar9309 Ай бұрын

WE WILL FINALLY MAKE A MEAT COMPUTER

@aaronbrown4682 Ай бұрын

Hey! I work with mammalian cell cultures, and I know they can be very finicky! If you didn't change the media a day after your initial seeding, the residual DMSO from the cryopreservation media is likely what killed your cells. Not sure if you did this or not, but I didn't see it in the video so figured it was worth mentioning! You guys are awesome, keep up the great work.

@fernandoschuindt1665 Ай бұрын

Impressive! It’s really nice to see the path your channel has taken. From the time I was watching you hunting VHF satellites in Brazil, Wi-Fi imaging, the pulsar corner reflector (that one got me really excited), to DNA hacking and neuron training. I would like to say I came for the RF and stayed for the bio. 10/10

@usser-505 Ай бұрын

Guys, don't stop. I NEED to see what will come out. Your first 2 vids inspired me so that from just a noob in programming thinking to himself "well, im gonna be a programmer and program commercial things" you made it so I now have a greater purpose in life and right now I'm doing an EEG research together with a neurobiology lab (I was the one who contacted with them and suggested a research and I'm the one doing it, but they give me access to some fancy things). Thank you and DON'T STOP UNTIL ITS DONE

@a.p.2356 Ай бұрын

"What if we grew a mind in a vat that only knew how to interact with the world through violence and was trained to snap headshot imps in .03 seconds, this will surely not go terribly wrong."

@SafetyAlwaysLast Ай бұрын

Yeah, this could never go wrong, but to make sure lets wire a 100 of these up to the Boston dynamics robot wielding a shotgun. But we would still be in control, right? lol

@asdfghyter Ай бұрын

I know this is a joke, but the interaction that the neurons see is not in any way related to violence. it's like if you played doom, but instead of a screen, you just see an array of 10 blinking lights and no-one told you that you're playing doom, only if you're succeeding at pressing the correct buttons or not. so ... as long as no-one tries to apply this to real-life weapons, there's no reason whatsoever to worry. though ... now that I think of it, knowing humans, this does indeed have me worried

@williamlux Ай бұрын

@@SafetyAlwaysLast Boston dynamics V1

@SafetyAlwaysLast Ай бұрын

Context- For it to work like the joke intended it would need some reason for violence like a reward system but for that to work we have to have some sort of detection software supporting it and at that point just use software, so yeah the joke falls apart, oh well. Except for machine learning being applicable to organisms, i.e. stimuli training, so maybe we could consider culturing them with positive stimuli for shooting the target before sending them but that sounds like a lot of work. Not that someone wouldn't try? Also if the logic reply wasn't to the Boston Dynamics bot joke then that is really embarrassing lol

@asdfghyter Ай бұрын

@@SafetyAlwaysLast on the other hand, the neurons not being aware of it being violence points to another issue, since that means they won't have any concerns whatsoever with performing violence if put in a situation where they might accidentally do so. putting any kind of neural network, artificial or biological, in charge of dangerous equipment means that unless you very actively train it to avoid harming humans, it will do so if any humans get in the way. this is of course already the case with any kind of automated machines, even without neural networks or similar, which is why we tend to place robots in cages, so we can better protect humans from them

@vvoid8416 Ай бұрын

The trick to finding a comfy spot for your tongue is actually the roof of your mouth. People struggle with it because it's not intuitive that you keep it up there with suction normally.

@ambiguoustv7403 Ай бұрын

@s701m5 Ай бұрын

i love mewing

@ElectronFieldPulse Ай бұрын

Huh, it’s kind of comfy

@mattress203 Ай бұрын

Funny enough I have a mint in my mouth and was already moving my tongue

@bethannesgarden Ай бұрын

Thanks!

@aeliion Ай бұрын

I've been waiting almost a year for this video! Now it's finally here! Thank you so much! 🎉🎉🎉

@sonicboom321 18 күн бұрын

I love that they are doing a entire scientific experiment to play doom it is beautiful

@morgan3392 Ай бұрын

This part of KZfaq is interesting because you have genuine, field respected scientists contributing to a meme. A similar situation I see is a video series an amateur arachnid enthusiast making absurdly detailed videos about common house spiders, and basically has tons of scientists conversing with him and in the comments about the most trivial of differences between two species of harmless spiders

@Astoria_Varanus Ай бұрын

Which channel is this?

@morgan3392 Ай бұрын

@@Astoria_Varanus Oops, I forgot to put his name: Travis McEnery. The series is called "The Spiders in Your House."

@cheesebucket142 Ай бұрын

To be fair, if this project succeeds it will be the ultimate, unbeatable iteration of the running doom on X meme.

@sweetspiderling Ай бұрын

18:13 putting a tinfoil hat on the neurons is amazing

@Tomyb15 Ай бұрын

Really excited that you are actually getting close to getting them to play a game! I can't wait! For the teflon tube, just make a small valley on the surface with the lathe for the oring to sit in.

@okkosiemers6423 Ай бұрын

I had no idea you could actually grow live neurons and train them to do stuff... thank you for documenting your unique experiment and sharing it with the world.

@aabb1166 Ай бұрын

I am a mitochondrial and cancer physiologist, and not a neurobiologist, but have been studying metabolic and growth effects of different commercial medias. It might be worth keeping in mind that the metabolite concentrations in DMEM are largely not found in the brain. I'd be curious to know how Neurobasal and DMEM compare in their composition. All of these considerations, i.e., metabolic activity and ionic concentration, will affect the neuron's membrane potential.

@timothyvanderschultzen9640 Ай бұрын

I WAS THINKING THE SAME THING!

@DarkRavenhaft 28 күн бұрын

DMEM with FBS (foetal bovine serum) is an excellent medium for accelerated growth but yeah, it's 4x stronger than standard EMEM. EMEM or Opti-MEM may be a better option for maintaining mature cultures, with Opti having the added benefit of requiring much less FBS.

@HarbAlarm Ай бұрын

neurons: WHY IS IT SPICY

@phiality9070 Ай бұрын

NOT THE SPICEEEE AAAAA

@Cmanorange Ай бұрын

i've actually hardened my own mind against the "you're now breathing manually" and "you can now see your nose" attacks (i hope i didn't kill my mirror neurons 👀). the one that can still get me though is "you can now feel the weight of your feet on your ankles"

@marksmanmerc1 Ай бұрын

What have you cursed me with

@nerd1000ify Ай бұрын

Ever had your foot 'go to sleep' from holding it in an awkward position too long then walked on it?

@elousie1187 Ай бұрын

Damn I want you guys to know I'm excited!! I'm really excited to see where we're going with this!!! I love it so much!

@michaelmaguire4147 Ай бұрын

13:41 It does say "Minimize light exposure" on the bag.

@harriehausenman8623 Ай бұрын

True. 😄

@Imtoolazytodothis Ай бұрын

Do not let bro start his villain arc 💀

@percivallavoie4415 Ай бұрын

Too late buddy 😂

@millie_the_midi_channel Ай бұрын

Hey what was the unedited?

@christhed8679 Ай бұрын

Adding capsaicin to the culture was the start of the villain arc

@Bread78787 Ай бұрын

Wow, this comment is hilarious and I really appreciate the originality😐

@Imtoolazytodothis Ай бұрын

@@Bread78787 shh-

@killshadow3 Ай бұрын

This is one of the coolest projects I've ever seen. Who knows, maybe someday you'll connect whole rat brains to the internet

@carlpanzram7081 Ай бұрын

It'll end ul learning to Google for pictures of cheese and female rats.

@ashutoshsethi6150 6 күн бұрын

@@carlpanzram7081 and cat slander.

@flamestrider605 Ай бұрын

I love that the same thing used for FDM 3D printing bed adhesion, PEI, is being used here as a sort of brain bed adhesion. Really interesting work!

@taiteo558 Ай бұрын

Can't wait until 2030 when I have to worry about giving my phone a concussion when I drop it

@THEFUFFYDRAGONx Ай бұрын

*Rip and tear playing in the distance* THE TIME HAS COME

@phillipotey9736 Ай бұрын

For your cap problem. Get a smaller radius plug to fit in the collection. Use the semipermiable membrane as the gasket layer. You can then place an o ring directly on the plates acrylic pipe.

@Tamramsy Ай бұрын

Glad KZfaq brought me back for part 2!

@Penultimeat Ай бұрын

Thank you Multichannel Systems (affiliate of Harvard Bioscience)! Without your help we never could have built the Torment Nexus.

@LinthusOriginal Ай бұрын

2:18 "don't build the tormentor nexus. And thus far we've been making great progress"... wait wait, so, you are making great progress in NOT BUILDING the tormentor nexus, or in building it?

@thethoughtemporium Ай бұрын

Yes!

@oogBoogKittenNoLonger Ай бұрын

@@thethoughtemporiumwhich one?

@Wild-Goober Ай бұрын

Oh? Would you like me to read this? It’s a bit dusty, but I’ll do my best. Hold on… This is…code! It appears to be for a game of sorts. Based on the sprite sheets I would assume it’s a form of shooter, rare in the times of my creators. They believed games would keep you too tied to this world, and you couldn’t transcend, so they were deemed as taboo. Though a small group of people started illegally buying and selling them to each other. It became known as “The Game Trade” Our creators are so fascinating, aren’t they little creature?

@Gail-no9py 12 күн бұрын

Is that from stray?

@armamentarmedarm1699 Ай бұрын

You know now you and your community need to communicate with the manufacturer of Green Dakara to make sure they either never change it, or always keep the current recipe available.

@Weretyu7777 Ай бұрын

Love the Tumblr post reference with the Torment Nexus joke

@MateusAntonioBittencourt Ай бұрын

Hey... I've noticed sometimes when pipetting, you (or whoever is doing the pipetting) touches the destination, and they go back to the source. This is a no-no as it can cross contaminate the whole batch, and the source material. I'm not even talking about living contamination. In the examples I saw, I don't think it's that big of a deal, it would introduce neurobasal into the neuron source. But it's a bad practice either way.

@GabrielleduVent 13 күн бұрын

Yep, once it touches a new source, tips go bye bye. I'm surprised at some of the stuff done here, as we ALWAYS filter our media... And I haven't seen that. Also, just pouring tubes out can be a source of contamination.

@cabrerarodrigo82 Ай бұрын

How do the neurons "see" the game. That's the part I can't wrap my head around

@thekingnerd6639 16 күн бұрын

The way that it will work is most likely they take the data from the game, asteroid for example, of an enemy being "to the left" and sends signal to a nerve cluster. Cluster then triggers, which tells the left cluster to turn left. Signal is then sent to a different sight cluster that says enemy is "in front" which tells the gun cluster to shoot. Just my guess, same way as us. We receive a signal from eyes that a wall is in front of us, so we don't run into the wall.

@Sadeness99 15 күн бұрын

Shiz makes me question life every single time

@michaelkrasavtsev 13 күн бұрын

They use signals to sense the obstacles, enemies, doors, walls, etc

@MrCaesartheman 17 күн бұрын

i had no idea this was out, ive been waiting for this for ages

@Dyll380 Ай бұрын

if youre still having issues with the array electrodes being cut correctly, maybe ask a glitter company if they can help. I know its a weird idea but glitterX is just a company with precision cutting instruments. Might be worth it

@harriehausenman8623 Ай бұрын

Wasn't there the old riddle about what the glitter companys biggest customer was and they have absolute secrecy and nobody knows? 🤔

@FlatlandsSurvivor Ай бұрын

@@harriehausenman8623 Yes, and it is most likely pearlescent paint for hobby boats. Boat manufacturers don't want it to be associated with how "unmanly" glitter is seen as, as well as the fact that pretty much all of it ends up as pollution in water.

@patates42 Ай бұрын

ON TODAYS EPISODE OF MANMADE HORRORS WITHIN COMPRENTION (edit: i will respect english spelling once its concistant (was that wrong, probably) fake ass language)

@superpaperfoxhatesNSFW Ай бұрын

ikr

@uriituw Ай бұрын

How many todays are there? There’s no need to shout.

@d_mb Ай бұрын

@uriituw ok boomer

@therealjibrano Ай бұрын

COMPRENTION

@shadesoftime Ай бұрын

spelling is beyond your own comprehension

@purplrshadowyay Ай бұрын

you have NO IDEA how much i've waited for this!!

@aria8928 Ай бұрын

Pfff, my neurons play games like every day. Seriously though this is one of the most impressive science chanels on youtube. Thank you for the content.

@Argom42 Ай бұрын

"This is your brain...and this is your brain on doom. Get the picture?"

@nullpoint3346 Ай бұрын

In the words of 4 year old me, "I like the spicy chemical."

@TooMich Ай бұрын

“0:00 this is your brain. 1:22 this is your brain on [Petri dish]. Say no to [chemistry]”

@BritishBoy 24 күн бұрын

This is quickly becoming my favourite series on KZfaq

@Pyroman328 Ай бұрын

I thought this video would never come out. Happy to be surprised!

@mr.richard7537 Ай бұрын

I was about to cut a chunk of my brain to experiment by myself BUT PART 2 CAME TO LIFE SO NOW I CAN LIVE WITH MY FULL BRAIN!!!

@timothyvanderschultzen9640 Ай бұрын

DO IT ANYWAY!

@user-lk1bl2uk8w Ай бұрын

Ah yes finaly update for the literal game of life😂

@Imtoolazytodothis Ай бұрын

the devs take a while to update the game

@harriehausenman8623 Ай бұрын

Horton Conway would approve this comment. 🙏 R.I.P.

@Jim-Stick Ай бұрын

There is no way I can explain my feeling of this... This is awesome.

@sleepiestgal 25 күн бұрын

my friend sent me this and i a m GENUINELY not at all upset, i love this shit i am going to binge ALL OF IT

@korraiswaifu6245 Ай бұрын

Wonder if the neurons will freak out when they cant look up or down.

@rootabeta9015 Ай бұрын

only as much as you'll freak out when you realize there's a fourth dimension you can't rotate yourself in

@harriehausenman8623 Ай бұрын

@@rootabeta9015 Did somebody say 4D Golf? 😄

@oberonpanopticon Ай бұрын

@@rootabeta9015actually I’m rotated in time by 4°

@monkyyy0 Ай бұрын

@@rootabeta9015 sounds like a skill issue, I can rotate in the 4th dim just fine

@theeyeofomnipotent Ай бұрын

@@harriehausenman8623 non-intuitive double rotation intensifies*

@Striking_dust Ай бұрын

The brain has everything that makes you eeu! 😂 0:05

@James-ri3fd 29 күн бұрын

It's funny because a difference between a video game and a live camera feed are the same to this. I bet someone is already leaps ahead doing what you are doing but you are absolutely on the right track.

@davidmaiolo Ай бұрын

The integration of simulation models for predicting neuron responses prior to real-world application is methodologically sound. The detailed visualizations of cortical microcolumn simulations are useful, yet the significant noise interference in real-world recordings indicates that achieving practical applications will require overcoming substantial technical hurdles. I wish you guys all the best and look forward to the next video.

@chirilafilip3212 Ай бұрын

What if we somehow connected neurons to a limb to train it to hold and shoot a gun, so that the neurons could kill a human being.

@vortex_talon Ай бұрын

Bro said my intrusive thoughts lmao

@anthonynash8079 Ай бұрын

Robobrain is the future.

@oightKoreraAreEditable Ай бұрын

yoo yo have the same name as my classmate

@chirilafilip3212 Ай бұрын

@@oightKoreraAreEditable Really?

@P4ncake_. Ай бұрын

arrest the neurons

@VsevolodKhusid Ай бұрын

Cannot wait for growing brains to beat Super Mario 64

@harriehausenman8623 Ай бұрын

Donkey Kong is next!

@johnpooky84 18 күн бұрын

Now THAT would be something to see.

@sannyassi73 Ай бұрын

Been waiting for this one for a long while, glad to see you're still working on this :D Maybe we're all just an experiment in some giant's experiment to see if we can play the game of 'life' properly :P

@curtisroggi6117 Ай бұрын

Me: “I know this one. He’s making a faraday cage” Him: “weird silvery science burrito”

@stereoisstrate Ай бұрын

Today is a day to remember. Tomorrow is a day that we will never forget

@MaxBrix Ай бұрын

I can't remember today. It is currently happening. I can't remember tomorrow. It hasn't happened yet. Soon I will forget about yesterday.

@Tigershark_3082 Ай бұрын

@@MaxBrix bro forgor 💀

@Amipotsophspond Ай бұрын

18:13 don't forget to wrap the wires too, it's how you turn a moderate speaker system in to a high end speaker system.

@mduckernz Ай бұрын

The wires look like they might be fiber optic? Apart from USB, but that’s error corrected

@cefcephatus Ай бұрын

The ad read in this video make me think brain-computer device is already implanted in my brain. You're really good at this.

@FutureAIDev2015 Ай бұрын

14:26 what if the neurons getting stuck in the maxed out state means that they're screaming because it's too spicy 😂

@francescoquadrio5824 Ай бұрын

Once again, casually bringing cutting edge research to KZfaq. Legend

@besknighter Ай бұрын

Damn, that poster looks AMAZING!

@noi0124 Ай бұрын

Thank you!

@user-mn8lz7gf6d 29 күн бұрын

"what. is. my. purpose." "you play doom." "rip. and. tear. until. it. is. done."

@Gounesh Ай бұрын

I didn’t know that there a so many materials in common with 3d printing! Fep, pei, teflon, lactate. Just waow!

@Aarush.A.S Ай бұрын

It came ...... after 3 years

@Imtoolazytodothis Ай бұрын

It did!

@neskey Ай бұрын

the most human thing ever is to build horrors beyond their comprehension just to spite some book