Connecting Living Neurons to a Computer | See Neurons Firing!

  Рет қаралды 542,255

The Thought Emporium

The Thought Emporium

16 күн бұрын

Use code thoughtemporium at the link below to get an exclusive 60% off an
annual Incogni plan: incogni.com/thoughtemporium
_________________________________________________________________________
Help support our work and get your poster here:
thethoughtemporium.ca/product...
Screen printed by La Bourgeoise Sérigraphe:
www.labourgeoiseserigraphe.co...
_________________________________________________________________________
Special thanks to Multichannel Systems for Providing the arrays and recording system! You can find their whole catalogue of arrays and tools here:
multichannelsystems.com/
________________________________________________________________________
Support the show and future projects:
Patreon: / thethoughtemporium
Nebula: go.nebula.tv/thethoughtemporium
Ko-Fi: ko-fi.com/thoughtemporium
Become a member: / @thethoughtemporium
Store: thethoughtemporium.ca/
________________________________________________________________________
More resources:
www.jove.com/t/2056/how-to-cu...
potterlab.gatech.edu/publicat...
journals.plos.org/ploscompbio...
www.nature.com/articles/s4192...
engineering.cmu.edu/news-even...
www.scholarpedia.org/article/S...
• Spiking Neural Network...
• The Cortical Column
_______________________________________________________________________
Previous Episode: • Growing Living Rat Neu...
First Try: • Growing Human Neurons ...
Meat Robot: • A Leaf Made of... Meat??
Meatcubator: • This Machine Grows Liv...
Meat in Gatorade: • Turning Gatorade Into ...
Plasma Metal Coating: • Metal Coating using PL...

Пікірлер: 2 300
@thethoughtemporium
@thethoughtemporium 13 күн бұрын
If you'd like to support our work and get yourself an array poster, check out our store: thethoughtemporium.ca/products/uv-glow-poster
@teemteem
@teemteem 13 күн бұрын
maybe
@Alceste_
@Alceste_ 12 күн бұрын
Did I get "printed locally here in Quebec" right? :o
@noi0124
@noi0124 12 күн бұрын
@@Alceste_ yes, the link to the screen print studio is in the infobox
@Chitose_
@Chitose_ 12 күн бұрын
*THE PART TWO WE HAVE ALL BEEN WAITING FOR* 🎉🎉🎉🎉🎉
@murch5054
@murch5054 11 күн бұрын
Hi, i think the link isn't working
@nl4006
@nl4006 14 күн бұрын
The reason the neurons all died after the initial seeding is because you didn't rinse the PEI off properly and/or didn't dry it properly. While PEI is the best pre-coating agent in the business, it is also an extremely potent membrane perforant when in solution, so any miniscule picogram of the stuff you left unpolymerized is gonna be jumping from cell to cell, shredding their membranes. Rinse at least five times with maximal volume your growth vessel can take, and dry the vessel overnight in the LAF bench, otherwise you're just wasting expensive reagents. Source: neuro lab-slave for 10+ years
@kevincuevas8877
@kevincuevas8877 14 күн бұрын
Bump and like for visibility
@sinedddmk8996
@sinedddmk8996 14 күн бұрын
Sounds like you are an ideal buddy for this dude to keep track of details, if you didnt have a job already.
@lucasmeneses8341
@lucasmeneses8341 14 күн бұрын
Up!
@esra_erimez
@esra_erimez 14 күн бұрын
I was about to say that exact some thing. However, you left out the part about the goop in the gloopinator. You really need to check that too.
@user-nb6mv3ny8i
@user-nb6mv3ny8i 14 күн бұрын
post doc in a university?
@hhhpestock951
@hhhpestock951 14 күн бұрын
"We're naming it: The Torment Nexus, after the hit book, Don't Build The Torment Nexus."
@spencerharrison9680
@spencerharrison9680 14 күн бұрын
Bro is literally a classic evil scientist at this point
@FateStayN1ght
@FateStayN1ght 14 күн бұрын
I cant stop laighting at that !
@harriehausenman8623
@harriehausenman8623 14 күн бұрын
@@rubikscuber9277 Absolutely! (in the classical meaning of the word, like in "The Gay Falcon" (1941)) 😄
@rubikscuber9277
@rubikscuber9277 14 күн бұрын
@@harriehausenman8623 sorry I mean to say Yay
@Otzkar
@Otzkar 14 күн бұрын
@@spencerharrison9680 *tech company
@aerofight5994
@aerofight5994 14 күн бұрын
Strange man in a sweaty lab coat staring at a rat in the corner of the room. Thinking to himself ... "Can it run DOOM?"
@ussuratoncachi
@ussuratoncachi 14 күн бұрын
More correctly: "Can it play DOOM?"
@mishtrong
@mishtrong 6 күн бұрын
​@@ussuratoncachiCan it RUN doom? Can I use its brain as a computer to run doom?
@Spartan11117777
@Spartan11117777 Күн бұрын
Can it run in a Hamster Wheel to power the Platform running Doom?
@joshcarriker6958
@joshcarriker6958 13 сағат бұрын
The body is just the case for the computer
@TheGrammarNazi123
@TheGrammarNazi123 7 сағат бұрын
"👍✌☠ ✋❄ ☼🕆☠ 👎⚐⚐💣✍"
@martyshrekster
@martyshrekster 13 күн бұрын
"Yeah I'm doing research on neural networks." "Oh, what algorithms are you working with?" "Rat"
@legenberry3217
@legenberry3217 10 күн бұрын
Underrated 😂😂
@denifnaf5874
@denifnaf5874 10 күн бұрын
Can it run doom?
@Beeti1
@Beeti1 6 күн бұрын
That's the medium, not the algorithm.
@Durei.rei_
@Durei.rei_ 14 күн бұрын
Imagine being conscious just to find out your purpose is to do a playthrough of doom
@kirralabas5315
@kirralabas5315 14 күн бұрын
Somehow that doesn't seem that bad, it's a decent life purpose
@AllanSchon
@AllanSchon 14 күн бұрын
That's more or less how I remember my high school years...
@colematlock7755
@colematlock7755 14 күн бұрын
A fulfilling life
@dercncplaner
@dercncplaner 14 күн бұрын
you know that there are theories that we live in a game. Soooo
@JohnDlugosz
@JohnDlugosz 14 күн бұрын
That's a lot better of a purpose than to pass the butter.
@orsonzedd
@orsonzedd 14 күн бұрын
I'm glad someone's finally getting around to building the torment Nexus
@ayybe7894
@ayybe7894 14 күн бұрын
Well it wasn't going to build itself.
@Imtoolazytodothis
@Imtoolazytodothis 14 күн бұрын
@@ayybe7894 your not wrong lmao
@LordDragox412
@LordDragox412 14 күн бұрын
Me: Wait, where does the "torment" come from in Torment Nexus? The Thought Emporium: Cayenne pepper flakes. Look at the neurons screaming in torment. Delightful. Me: Oh yeah, that makes total sense, thanks.
@jakobweisser8044
@jakobweisser8044 14 күн бұрын
I'm just glad it's the humble mad scientist and not the techbros.
@warmitchine3388
@warmitchine3388 14 күн бұрын
@@jakobweisser8044 "We built a neural interface on an unregistered cruise liner off the coast of Panama that mines cryptocurrency. We just need your donation of $200,000 to keep it running and to pay the illegal waste dumping fees." -techbro neuroscientists
@jackhazardous4008
@jackhazardous4008 13 күн бұрын
>wake up >your entire existence is DOOM >pass on thinking that was all there ever was to life
@ghoulbuster1
@ghoulbuster1 13 күн бұрын
Same as it ever was.
@poupeuu
@poupeuu 12 күн бұрын
>wake up >your existence is this one >pass on thinking that was all there was to life
@jeremymcadam7400
@jeremymcadam7400 12 күн бұрын
​@@poupeuu This is why I choose to participate in 5 dimensions
@gondolaone7748
@gondolaone7748 8 күн бұрын
​@@jeremymcadam7400 rookie numbers, all of us in the 6th dimension are looking down on your struggles, purely for amusement I might add
@j0rtsy
@j0rtsy 5 күн бұрын
Plato’s “The Cave” is Doom.
@X-SPONGED
@X-SPONGED 11 күн бұрын
3:20 "You merely adopted the sports drink. I was BORN in it, MOLDED by it"
@kayjersch4959
@kayjersch4959 14 күн бұрын
3:50 "long term, i want to simulate a whole brain" fucking WHAT
@thewizard1
@thewizard1 14 күн бұрын
Bro is a mad scientist 😭
@harriehausenman8623
@harriehausenman8623 14 күн бұрын
Naturally. 🧐
@samuelhomberg9075
@samuelhomberg9075 14 күн бұрын
I think he's not talking about a human brain (so far...).
@CarlosSpicyWang
@CarlosSpicyWang 14 күн бұрын
@@samuelhomberg9075You want skynet? That’s how you get skynet.
@wesleymays1931
@wesleymays1931 14 күн бұрын
​@@samuelhomberg9075give him time...
@hakajiru264
@hakajiru264 14 күн бұрын
Painstakingly growing a brain slice only to pepperspray it moments later -.-
@ayybe7894
@ayybe7894 14 күн бұрын
Such is the cruel nature of life
@LordDragox412
@LordDragox412 14 күн бұрын
Do not build the Torment Nexus. But if you do, do not torment the Torment Nexus with cayenne pepper flakes.
@Imtoolazytodothis
@Imtoolazytodothis 14 күн бұрын
Sounds like a middle-age crisis
@harriehausenman8623
@harriehausenman8623 14 күн бұрын
Almost human
@Asmodis4
@Asmodis4 14 күн бұрын
every time i found one of his videos i get this harlan ellison vipes and i dont know why.
@isodoublet
@isodoublet 13 күн бұрын
Don't you just hate it when you're chilling in the torment nexus when all of a sudden your entire being is overcome with an extremely powerful chemical irritant?
@SophieYui
@SophieYui 14 күн бұрын
Imagine being the neurons "okay, so we can't sense anything, and we probably can't do anything, but we AAH SPICY!"
@thecoolestofguys
@thecoolestofguys 12 күн бұрын
the torment nexus will be built!
@harrisonlichtenberg3162
@harrisonlichtenberg3162 11 күн бұрын
Homie really dumped hot chem on raw nerves just to see the funny lights
@rubyred186
@rubyred186 11 күн бұрын
I have no mouth and I must scream.
@matturner6890
@matturner6890 8 күн бұрын
you're all replying to a bot, pay attention.
@Dann.y
@Dann.y 6 күн бұрын
​@@matturner6890WE ALL KNOW! AND DID YOU KNOW THAT YOU ARE REPLYING TO HIM TOO?!
@nuclearocean
@nuclearocean 14 күн бұрын
Can't wait till I have to feed my computer and overclock it with some spicy red pepper
@henrysanecdotes5323
@henrysanecdotes5323 14 күн бұрын
*losing in valorant* MY FRAMES! GET THE PEPPER
@AngryAlfonse
@AngryAlfonse 14 күн бұрын
"Hey guys, my GPU is struggling to hit 120fps. Gotta go toss a 5 hour energy into the growth medium circulator."
@alephnole7009
@alephnole7009 14 күн бұрын
Sprinkling it in like fish food into a brain in a tank
@harriehausenman8623
@harriehausenman8623 14 күн бұрын
*playing fps game* *loosing connection* "Guys, my machine just had a seizure."
@navinvent
@navinvent 14 күн бұрын
What i learnt is that injecting pepper into the brain will be an effective form of torture.
@anonymousapproximation8549
@anonymousapproximation8549 14 күн бұрын
"It's designed for researchers, not a bunch of crazy nerds" "Corporate needs you to spot the difference between these images"
@mihael64
@mihael64 12 күн бұрын
Researchers aren't nerds, they're just crazy
@ttty2242
@ttty2242 12 күн бұрын
budget
@SioxerNikita
@SioxerNikita 11 күн бұрын
@@mihael64 But... they are also usually nerds XD
@vidal9747
@vidal9747 10 күн бұрын
@@mihael64 My advisor literally can not physically stop himself from talking about Dune if he drank more than 2 beers... And not every researcher is crazy. Some can even manage work life balance. Not anyone I know, but I heard some can!
@blakksheep736
@blakksheep736 7 күн бұрын
The only difference between a researcher and a nerd is that researchers are paid.
@christophepetry6782
@christophepetry6782 13 күн бұрын
I'm a doctoral researcher and Neuroscientist. When it comes to culturing medium for neurons morphology can tell you a lot about the state a cell is in (this goes for most cell types). To me the classic choice of Neurobasal gives you cells with shrunk cell bodies (the nucleus is tightly shrink wrapped if you will) and long complex truncated neurites (dendrites and axons). This tells you that these cells are "mature" neurons. They are not quite mature because you literally need let time pass for them to become mature, interestingly the time of maturation depends on species and is much longer for human neurons compared to mouse neurons.
@TheAmazingAnimator
@TheAmazingAnimator 9 сағат бұрын
I think that's because rats have less neurons to mature overrall. also because other animals (I think) gain consciousness faster
@christophepetry6782
@christophepetry6782 3 сағат бұрын
@@TheAmazingAnimator it is more about complexity of their connections and morphology and less about the overall number of cells. In culture you can observe the same time discrepancies between neurons of different species, so it is an inherent quality of the cells. We are not clear on what level of consciousness other animals have , since we can’t test for it. You probably mean animals become independent much earlier, since humans literally complete their development outside the mothers body. During evolution our heads got to big for our pelvises so we have to be born “prematurely”.
@professorstevepotter
@professorstevepotter 11 күн бұрын
9:55 Aha! apropos to my previous comment about cell density, I see how to solve your problems: We always added a small droplet (50-100ul) of cell suspension added to an EMPTY dish, with just a 3mm laminin wet spot. Calculate what density you need in the suspension to get 50k-100k cells in that droplet. After the cells have settled and adhered (30 min?) carefully add the rest of the medium to flood the dish. Now you will have very dense cultures and signals on every electrode.
@MyDadSaid
@MyDadSaid 11 күн бұрын
Thank you. We really appreciate all your input.
@davef21370
@davef21370 14 күн бұрын
It's brilliant but a little sad to think that a dish full of rat neurons could potentially beat me at Doom.
@breadboi3837
@breadboi3837 14 күн бұрын
the ultimate cheat system, full automation cheats; I could grind war thunder off a chunk of rat brain
@zachrowe6271
@zachrowe6271 14 күн бұрын
​@@breadboi3837imagine getting banned for rat brain botting 😂
@harriehausenman8623
@harriehausenman8623 14 күн бұрын
@@zachrowe6271 🤗
@user-burner
@user-burner 14 күн бұрын
To be fair, you also have to think about, yknow, having a body and pressing keys and shit.
@NiclasOvik
@NiclasOvik 14 күн бұрын
​@@user-burnerTrue that, all about focus, reaching flow state.
@SophieYui
@SophieYui 14 күн бұрын
"Slightly sentient enchilada" caught me off guard xDDDD
@steamerpowered
@steamerpowered 12 күн бұрын
Yup, not a sentence I expected to hear this morning... lol
@nullpoint3346
@nullpoint3346 9 күн бұрын
It's only sentient, we're fine _(for now)._
@matturner6890
@matturner6890 8 күн бұрын
you're all replying to a bot, pay more attention.
@nullpoint3346
@nullpoint3346 7 күн бұрын
@@matturner6890 Do you think we care?
@BionicleFreek99
@BionicleFreek99 13 күн бұрын
Finally! Man-made horrors WITHIN my comprehension!
@Mechthorian
@Mechthorian 14 күн бұрын
24:40 Surely this is the worst O-Ring disaster in the scientific profession...
@noi0124
@noi0124 14 күн бұрын
New challenger appeared!
@AresHoax_9
@AresHoax_9 14 күн бұрын
@@noi0124it’s the challenger space shuttle ! Ready? Set Fight!
@harriehausenman8623
@harriehausenman8623 14 күн бұрын
Maybe they just have to chill… *checking notes* On no, wait. That was NOT the cure 😆
@JinKee
@JinKee 14 күн бұрын
Too soon
@m4rcyonstation93
@m4rcyonstation93 13 күн бұрын
Context? (Curious)
@RevolutionPony
@RevolutionPony 14 күн бұрын
I loved reading "Don't Build the Torment Nexus!" When I was a kid. I don't remember what the moral of the story was, but I really liked the lore, aesthetics, and world building. I'm so glad someone is bringing my favourite sci-fi to life! We really do live in the future!
@Penguinman2.0
@Penguinman2.0 14 күн бұрын
Wait I can’t tell if this is genuine or not
@DawnPetrichor1
@DawnPetrichor1 14 күн бұрын
@@Penguinman2.0Neither can I… On a second reading, I don’t think it’s genuine, “I don’t remember what the moral of the story was” seems like it wouldn’t be there if it was.
@SissypheanCatboy
@SissypheanCatboy 14 күн бұрын
@@Penguinman2.0 The book doesn't exist, its from a meme talking about how techbros proudly claim inspiration from scifi stories where the whole moral is NOT to build the technology featured in the movie. Like how vr/metaverse nerds constantly say "its like the Matrix in real life"
@adora_was_taken
@adora_was_taken 14 күн бұрын
@@SissypheanCatboy or more recently, openai comparing gpt-4o to the movie "her"
@vipture.
@vipture. 14 күн бұрын
​@@SissypheanCatboyThe first thing we did after GPT-1 showed promise was hook up the next version to the internet to learn from. It's like the people at the forefront of advancement have turned dystopian prophecies into a checklist..
@AcceptableAsGenerallyDecent
@AcceptableAsGenerallyDecent 13 күн бұрын
dr stone would probably accomplish this with the power of jump cuts and eye closeups
@noi0124
@noi0124 13 күн бұрын
Power of anime
@cefcephatus
@cefcephatus 9 күн бұрын
9:29 "Why those ingredients? I have NO IDEA!" Yep! That's how you know someone is doing real science and not just teaching you prepared lesson.
@Rosencrantz17
@Rosencrantz17 14 күн бұрын
"What is my purpose?" "You play DOOM." "RIP AND TEAR! RIP AND TEAR! RIP AND TEAR!"
@Hexawatt
@Hexawatt 6 күн бұрын
Rip And Tear, or R.A.T. for short.
@Cacowninja
@Cacowninja Күн бұрын
Until it's done!
@Penultimeat
@Penultimeat 14 күн бұрын
Thank you Multichannel Systems (affiliate of Harvard Bioscience)! Without your help we never could have built the Torment Nexus.
@Prucake
@Prucake 13 күн бұрын
"Don't build the torment nexus" YOU HAD ONE INSTRUCTION.
@gevelegian
@gevelegian 14 күн бұрын
If youtube was just this type of content but with every expertise/experiment currently going on. It would be just perfect harmony.
@Imtoolazytodothis
@Imtoolazytodothis 14 күн бұрын
The perfect combination of long-form content, diligent and passionate work, and humor!
@LordDragox412
@LordDragox412 14 күн бұрын
Meanwhile KZfaq: Demonetized.
@aaronbrown4682
@aaronbrown4682 14 күн бұрын
Hey! I work with mammalian cell cultures, and I know they can be very finicky! If you didn't change the media a day after your initial seeding, the residual DMSO from the cryopreservation media is likely what killed your cells. Not sure if you did this or not, but I didn't see it in the video so figured it was worth mentioning! You guys are awesome, keep up the great work.
@professorstevepotter
@professorstevepotter 10 күн бұрын
My other suggestions seem to have been deleted, perhaps because I linked a paper of ours. I guessed that you may be killing cells with too much light. Phototoxicity is a huge problem with any fluorescence microscopy. To reduce it, use phase contrast (not fluorescence) microscopy and use longer-wavelength (red) light. Also, when doing fluorescence imaging, use a lower-NA lens, more sensitive cameras, dimmer illumination, longer wavelength excitation, and shorter exposure to light.
@thethoughtemporium
@thethoughtemporium 10 күн бұрын
Feel free to send me an email with all of the suggestions so they don't get lost! And they're greatly appreciated as always. We only use phase contrast when checking on the cells. Fluorescence was only used at the end for the sake of the experiment, and the dye was only used on one of the arrays after we had finished all other experiments. The arrays are also only ever left on the microscope for as brief a moment as possible, but the phase contrast images aren't as pretty so I don't use that footage in the video as much. I also saw you recommend concentrating the neurons in the middle. I read that in the instructions and that's how we attempted it the first time when the cells died. We went with adding the neurons after the media because we knew for sure the neurons would survive as we've tested that method a lot and couldn't risk another culture dying. We were pressed for time to get the video out. And for our arrays the electrodes are REALLY spread out, so we're gonna need a high neuron density across most of the surface of the array anyway. You'll notice I made the walls of our arrays taller than the multichannel ones. It was specifically because I wanted to increase the amount of growth media per well so the neurons don't deplete it as quickly and to account for an eventual much higher neuron density across the array. We have wanted to upgrade our camera on the microscope for a while, but the scope itself was a HUGE upgrade. It's new for this video and it cost a pretty penny. But it's built exactly for this kind of experiment so we can switch out the camera next. I also want to move to a red dye to lower the illumination wavelength as you say. And also just to make the videos look more interesting than always green. We ran out of time, but we also got a tubulin specific dye we're gonna show off next time that would look best against an otherwise red stained neuron. And we picked up NucBlue so we can stain the nucleus properly. One of the shots of the neurons firing included it from the first well we tested it on. I overlaid it. It's subtle but makes the neurons look nicer and the nucleus visible.
@thethoughtemporium
@thethoughtemporium 10 күн бұрын
Also, I've set your account to always show comments on the channel now, so they shouldn't get deleted.
@Amipotsophspond
@Amipotsophspond 14 күн бұрын
18:13 don't forget to wrap the wires too, it's how you turn a moderate speaker system in to a high end speaker system.
@mduckernz
@mduckernz 14 күн бұрын
The wires look like they might be fiber optic? Apart from USB, but that’s error corrected
@darealsherlock8026
@darealsherlock8026 14 күн бұрын
DOCTOR STEVE POTTER CONTACTED YOU! THE DOCTOR POTTER! This is surely indicative of success to come
@akatsukilevi
@akatsukilevi 14 күн бұрын
For the ones randomly watching this and has no idea who Steve Potter is and why it is a big deal It is as if Jesus Christ himself showed up for a theology class
@Imtoolazytodothis
@Imtoolazytodothis 14 күн бұрын
A truly impressive feat!
@harriehausenman8623
@harriehausenman8623 14 күн бұрын
What a Legend.
@jimcobbler3954
@jimcobbler3954 14 күн бұрын
I’m sure he’s never heard this joke, but he sounds like a wizard in this field.
@TekedixXx
@TekedixXx 14 күн бұрын
@@akatsukilevi I can't not stop thinking about Professor brothers now lol
@hakajiru264
@hakajiru264 14 күн бұрын
Neuron: Help I'm peeling Neurobiologist: SUGAR FREE HORSE JUICE
@thingyjim
@thingyjim 12 күн бұрын
Hey! I’m messing with neurons at the moment in my PhD and I saw the exact same morphology in DMEM/F12, I was stumped what was causing it! As a precaution we’re genotyping with ICC and qPCR since we’re deriving from iPSCs - anyway, love the video! This project is so cool!
@katofmine
@katofmine 12 күн бұрын
18:09 “slightly sentient enchilada” made me inhale half my beer
@TornaitSuperBird
@TornaitSuperBird 14 күн бұрын
10:09 "Hey how are the neurons growing?" "They all died" *AD* ...had a hard time trying not to chuckle in the doctor's office.
@WurliMonkhaven
@WurliMonkhaven 14 күн бұрын
Hope the appointment went well! God bless!
@jvdos
@jvdos 14 күн бұрын
THE DAY HAS COME BROTHERS! 🗣️🔥🔥
@Log4Jake
@Log4Jake 14 күн бұрын
FINNNNALLLLLYYYYY
@n0o0b090lv
@n0o0b090lv 14 күн бұрын
LETS GOOOOOOO💯💯
@Xxrasierklinge7
@Xxrasierklinge7 14 күн бұрын
Ikr! I've been waiting for this for what seems like FOREVER!!!!!
@lopatoj
@lopatoj 14 күн бұрын
yay
@quentar9309
@quentar9309 14 күн бұрын
WE WILL FINALLY MAKE A MEAT COMPUTER
@LinthusOriginal
@LinthusOriginal 14 күн бұрын
2:18 "don't build the tormentor nexus. And thus far we've been making great progress"... wait wait, so, you are making great progress in NOT BUILDING the tormentor nexus, or in building it?
@thethoughtemporium
@thethoughtemporium 14 күн бұрын
Yes!
@oogBoogKittenNoLonger
@oogBoogKittenNoLonger 13 күн бұрын
@@thethoughtemporiumwhich one?
@TooMich
@TooMich 14 күн бұрын
“0:00 this is your brain. 1:22 this is your brain on [Petri dish]. Say no to [chemistry]”
@aabb1166
@aabb1166 14 күн бұрын
I am a mitochondrial and cancer physiologist, and not a neurobiologist, but have been studying metabolic and growth effects of different commercial medias. It might be worth keeping in mind that the metabolite concentrations in DMEM are largely not found in the brain. I'd be curious to know how Neurobasal and DMEM compare in their composition. All of these considerations, i.e., metabolic activity and ionic concentration, will affect the neuron's membrane potential.
@timothyvanderschultzen9640
@timothyvanderschultzen9640 14 күн бұрын
I WAS THINKING THE SAME THING!
@DarkRavenhaft
@DarkRavenhaft 5 күн бұрын
DMEM with FBS (foetal bovine serum) is an excellent medium for accelerated growth but yeah, it's 4x stronger than standard EMEM. EMEM or Opti-MEM may be a better option for maintaining mature cultures, with Opti having the added benefit of requiring much less FBS.
@morgan3392
@morgan3392 14 күн бұрын
This part of KZfaq is interesting because you have genuine, field respected scientists contributing to a meme. A similar situation I see is a video series an amateur arachnid enthusiast making absurdly detailed videos about common house spiders, and basically has tons of scientists conversing with him and in the comments about the most trivial of differences between two species of harmless spiders
@Astoria_Varanus
@Astoria_Varanus 14 күн бұрын
Which channel is this?
@morgan3392
@morgan3392 14 күн бұрын
@@Astoria_Varanus Oops, I forgot to put his name: Travis McEnery. The series is called "The Spiders in Your House."
@cheesebucket142
@cheesebucket142 14 күн бұрын
To be fair, if this project succeeds it will be the ultimate, unbeatable iteration of the running doom on X meme.
@fernandoschuindt1665
@fernandoschuindt1665 12 күн бұрын
Impressive! It’s really nice to see the path your channel has taken. From the time I was watching you hunting VHF satellites in Brazil, Wi-Fi imaging, the pulsar corner reflector (that one got me really excited), to DNA hacking and neuron training. I would like to say I came for the RF and stayed for the bio. 10/10
@BritishBoy
@BritishBoy 23 сағат бұрын
This is quickly becoming my favourite series on KZfaq
@michaelmaguire4147
@michaelmaguire4147 14 күн бұрын
13:41 It does say "Minimize light exposure" on the bag.
@harriehausenman8623
@harriehausenman8623 14 күн бұрын
True. 😄
@titusbaum9690
@titusbaum9690 14 күн бұрын
Neuron-piloted flesh mechs growing neurons, simulating neurons, looking at neurons, visualizing neurons. Ah yes, Neuron Activation.
@audriusskebas3099
@audriusskebas3099 14 күн бұрын
Soon we shall have a flesh automaton animated by neurotransmitters!
@Gakulon
@Gakulon 14 күн бұрын
Do not dare to insult the purity of steel by comparing mechs to abominable flesh
@Mate_Antal_Zoltan
@Mate_Antal_Zoltan 14 күн бұрын
Bro just called God's greatest creation "neuron-piloted flesh mechs"
@audriusskebas3099
@audriusskebas3099 14 күн бұрын
@@Mate_Antal_Zoltan It's a Cruelty Squad reference xP
@Mate_Antal_Zoltan
@Mate_Antal_Zoltan 14 күн бұрын
@@audriusskebas3099 guy who has only played Cruelty Squad in his entire life: "I'm getting real Cruelty Squad vibes from this..."
@Tamramsy
@Tamramsy 12 күн бұрын
Glad KZfaq brought me back for part 2!
@wolfy2921
@wolfy2921 13 күн бұрын
Dude casually cultured neurons to make them have a spicy drink
@a.p.2356
@a.p.2356 14 күн бұрын
"What if we grew a mind in a vat that only knew how to interact with the world through violence and was trained to snap headshot imps in .03 seconds, this will surely not go terribly wrong."
@SafetyAlwaysLast
@SafetyAlwaysLast 13 күн бұрын
Yeah, this could never go wrong, but to make sure lets wire a 100 of these up to the Boston dynamics robot wielding a shotgun. But we would still be in control, right? lol
@asdfghyter
@asdfghyter 13 күн бұрын
I know this is a joke, but the interaction that the neurons see is not in any way related to violence. it's like if you played doom, but instead of a screen, you just see an array of 10 blinking lights and no-one told you that you're playing doom, only if you're succeeding at pressing the correct buttons or not. so ... as long as no-one tries to apply this to real-life weapons, there's no reason whatsoever to worry. though ... now that I think of it, knowing humans, this does indeed have me worried
@williamlux
@williamlux 12 күн бұрын
​@@SafetyAlwaysLast Boston dynamics V1
@SafetyAlwaysLast
@SafetyAlwaysLast 12 күн бұрын
Context- For it to work like the joke intended it would need some reason for violence like a reward system but for that to work we have to have some sort of detection software supporting it and at that point just use software, so yeah the joke falls apart, oh well. Except for machine learning being applicable to organisms, i.e. stimuli training, so maybe we could consider culturing them with positive stimuli for shooting the target before sending them but that sounds like a lot of work. Not that someone wouldn't try? Also if the logic reply wasn't to the Boston Dynamics bot joke then that is really embarrassing lol
@asdfghyter
@asdfghyter 12 күн бұрын
@@SafetyAlwaysLast on the other hand, the neurons not being aware of it being violence points to another issue, since that means they won't have any concerns whatsoever with performing violence if put in a situation where they might accidentally do so. putting any kind of neural network, artificial or biological, in charge of dangerous equipment means that unless you very actively train it to avoid harming humans, it will do so if any humans get in the way. this is of course already the case with any kind of automated machines, even without neural networks or similar, which is why we tend to place robots in cages, so we can better protect humans from them
@vvoid8416
@vvoid8416 14 күн бұрын
The trick to finding a comfy spot for your tongue is actually the roof of your mouth. People struggle with it because it's not intuitive that you keep it up there with suction normally.
@ambiguoustv7403
@ambiguoustv7403 14 күн бұрын
μ
@s701m5
@s701m5 14 күн бұрын
i love mewing
@ElectronFieldPulse
@ElectronFieldPulse 14 күн бұрын
Huh, it’s kind of comfy
@mattress203
@mattress203 14 күн бұрын
Funny enough I have a mint in my mouth and was already moving my tongue
@bethannesgarden
@bethannesgarden 14 күн бұрын
Thanks!
@user-mn8lz7gf6d
@user-mn8lz7gf6d 5 күн бұрын
"what. is. my. purpose." "you play doom." "rip. and. tear. until. it. is. done."
@phillipotey9736
@phillipotey9736 12 күн бұрын
For your cap problem. Get a smaller radius plug to fit in the collection. Use the semipermiable membrane as the gasket layer. You can then place an o ring directly on the plates acrylic pipe.
@MateusAntonioBittencourt
@MateusAntonioBittencourt 14 күн бұрын
Hey... I've noticed sometimes when pipetting, you (or whoever is doing the pipetting) touches the destination, and they go back to the source. This is a no-no as it can cross contaminate the whole batch, and the source material. I'm not even talking about living contamination. In the examples I saw, I don't think it's that big of a deal, it would introduce neurobasal into the neuron source. But it's a bad practice either way.
@sweetspiderling
@sweetspiderling 14 күн бұрын
18:13 putting a tinfoil hat on the neurons is amazing
@Gounesh
@Gounesh 11 күн бұрын
I didn’t know that there a so many materials in common with 3d printing! Fep, pei, teflon, lactate. Just waow!
@CactusMaractus
@CactusMaractus Күн бұрын
you know, when it's all laid out like this, it makes it sound simple
@HarbAlarm
@HarbAlarm 14 күн бұрын
neurons: WHY IS IT SPICY
@phiality9070
@phiality9070 12 күн бұрын
NOT THE SPICEEEE AAAAA
@THEFUFFYDRAGONx
@THEFUFFYDRAGONx 14 күн бұрын
*Rip and tear playing in the distance* THE TIME HAS COME
@aeliion
@aeliion 11 күн бұрын
I've been waiting almost a year for this video! Now it's finally here! Thank you so much! 🎉🎉🎉
@hugacreeper69110
@hugacreeper69110 13 күн бұрын
yesss been waiting for part 2 thank you
@korraiswaifu6245
@korraiswaifu6245 14 күн бұрын
Wonder if the neurons will freak out when they cant look up or down.
@rootabeta9015
@rootabeta9015 14 күн бұрын
only as much as you'll freak out when you realize there's a fourth dimension you can't rotate yourself in
@harriehausenman8623
@harriehausenman8623 14 күн бұрын
@@rootabeta9015 Did somebody say 4D Golf? 😄
@oberonpanopticon
@oberonpanopticon 14 күн бұрын
@@rootabeta9015actually I’m rotated in time by 4°
@monkyyy0
@monkyyy0 14 күн бұрын
@@rootabeta9015 sounds like a skill issue, I can rotate in the 4th dim just fine
@theeyeofomnipotent
@theeyeofomnipotent 14 күн бұрын
​@@harriehausenman8623 non-intuitive double rotation intensifies*
@Dyll380
@Dyll380 14 күн бұрын
if youre still having issues with the array electrodes being cut correctly, maybe ask a glitter company if they can help. I know its a weird idea but glitterX is just a company with precision cutting instruments. Might be worth it
@harriehausenman8623
@harriehausenman8623 14 күн бұрын
Wasn't there the old riddle about what the glitter companys biggest customer was and they have absolute secrecy and nobody knows? 🤔
@FlatlandsSurvivor
@FlatlandsSurvivor 14 күн бұрын
​@@harriehausenman8623 Yes, and it is most likely pearlescent paint for hobby boats. Boat manufacturers don't want it to be associated with how "unmanly" glitter is seen as, as well as the fact that pretty much all of it ends up as pollution in water.
@Pyroman328
@Pyroman328 13 күн бұрын
I thought this video would never come out. Happy to be surprised!
@mysticthecatninja
@mysticthecatninja 14 күн бұрын
This channel makes me so happy I love coming along to do science with you
@Spearhead-ke8kd
@Spearhead-ke8kd 14 күн бұрын
1:14 "...Is there a limit..." There's a Let's Game It Out 🤝 Thought Emporium. Terrifying people by saying "is there a limit."
@henrysanecdotes5323
@henrysanecdotes5323 14 күн бұрын
oh my gosh what a small world
@lorrainebrunner2490
@lorrainebrunner2490 14 күн бұрын
THIS. This one right here. ✅️
@pianovii3350
@pianovii3350 14 күн бұрын
"Let's find out!"
@SrirachaChugChallenge747-jq7by
@SrirachaChugChallenge747-jq7by 14 күн бұрын
Hold, please!
@xaphok2173
@xaphok2173 14 күн бұрын
Only one way to find out
@Weretyu7777
@Weretyu7777 14 күн бұрын
Love the Tumblr post reference with the Torment Nexus joke
@cefcephatus
@cefcephatus 9 күн бұрын
The ad read in this video make me think brain-computer device is already implanted in my brain. You're really good at this.
@GIBBEHGaming
@GIBBEHGaming 14 күн бұрын
IVE BEEN WAITING FOR THIS VIDEO! I gotta go rewatch the first episode again to get the full effect haha!
@taiteo558
@taiteo558 14 күн бұрын
Can't wait until 2030 when I have to worry about giving my phone a concussion when I drop it
@patates42
@patates42 14 күн бұрын
ON TODAYS EPISODE OF MANMADE HORRORS WITHIN COMPRENTION (edit: i will respect english spelling once its concistant (was that wrong, probably) fake ass language)
@foxguysumthing
@foxguysumthing 14 күн бұрын
ikr
@uriituw
@uriituw 14 күн бұрын
How many todays are there? There’s no need to shout.
@d_mb
@d_mb 14 күн бұрын
@uriituw ok boomer
@therealjibrano
@therealjibrano 14 күн бұрын
COMPRENTION
@shadesoftime
@shadesoftime 12 күн бұрын
spelling is beyond your own comprehension
@bfi01youtube11
@bfi01youtube11 14 күн бұрын
Your ability to explain the complex minutia of the process in an accessible and understandable way is phenomenal :) This is an awesome study!
@NobodyYouKnow01
@NobodyYouKnow01 14 күн бұрын
I keep having to rewatch the old videos to keep up with the story. And then watching the other videos for the side projects.
@francescoquadrio5824
@francescoquadrio5824 14 күн бұрын
Once again, casually bringing cutting edge research to KZfaq. Legend
@user-lk1bl2uk8w
@user-lk1bl2uk8w 14 күн бұрын
Ah yes finaly update for the literal game of life😂
@Imtoolazytodothis
@Imtoolazytodothis 14 күн бұрын
the devs take a while to update the game
@harriehausenman8623
@harriehausenman8623 14 күн бұрын
Horton Conway would approve this comment. 🙏 R.I.P.
@purplrshadowyay
@purplrshadowyay 13 күн бұрын
you have NO IDEA how much i've waited for this!!
@erezdude123
@erezdude123 12 күн бұрын
You are one of the main reasons I am perusing my masters in synthetic biology!!!! I love your videos man hope you keep doing what you do :)
@besknighter
@besknighter 14 күн бұрын
Damn, that poster looks AMAZING!
@noi0124
@noi0124 14 күн бұрын
Thank you!
@stereoisstrate
@stereoisstrate 14 күн бұрын
Today is a day to remember. Tomorrow is a day that we will never forget
@MaxBrix
@MaxBrix 13 күн бұрын
I can't remember today. It is currently happening. I can't remember tomorrow. It hasn't happened yet. Soon I will forget about yesterday.
@Tigershark_3082
@Tigershark_3082 10 күн бұрын
​@@MaxBrix bro forgor 💀
@casimircudinski7325
@casimircudinski7325 14 күн бұрын
I absolutely love this series
@sleepiestgal
@sleepiestgal 2 күн бұрын
my friend sent me this and i a m GENUINELY not at all upset, i love this shit i am going to binge ALL OF IT
@patrickthomas6985
@patrickthomas6985 14 күн бұрын
And we're back on the super villain type beat
@Imtoolazytodothis
@Imtoolazytodothis 14 күн бұрын
Do not let bro start his villain arc 💀
@percivallavoie4415
@percivallavoie4415 14 күн бұрын
Too late buddy 😂
@millie_the_midi_channel
@millie_the_midi_channel 14 күн бұрын
Hey what was the unedited?
@christhed8679
@christhed8679 14 күн бұрын
Adding capsaicin to the culture was the start of the villain arc
@Bread78787
@Bread78787 14 күн бұрын
Wow, this comment is hilarious and I really appreciate the originality😐
@Imtoolazytodothis
@Imtoolazytodothis 14 күн бұрын
@@Bread78787 shh-
@dansamarco1610
@dansamarco1610 13 күн бұрын
YESSS, I've waited so long for this!
@kylebowles9820
@kylebowles9820 12 күн бұрын
This is why I love this channel
@Aarush.A.S
@Aarush.A.S 14 күн бұрын
It came ...... after 3 years
@Imtoolazytodothis
@Imtoolazytodothis 14 күн бұрын
It did!
@Striking_dust
@Striking_dust 14 күн бұрын
The brain has everything that makes you eeu! 😂 0:05
@elousie1187
@elousie1187 13 күн бұрын
Damn I want you guys to know I'm excited!! I'm really excited to see where we're going with this!!! I love it so much!
@xplicit2734
@xplicit2734 13 күн бұрын
Been waiting for this vid!
@chirilafilip3212
@chirilafilip3212 14 күн бұрын
What if we somehow connected neurons to a limb to train it to hold and shoot a gun, so that the neurons could kill a human being.
@vortex_talon
@vortex_talon 14 күн бұрын
Bro said my intrusive thoughts lmao
@anthonynash8079
@anthonynash8079 14 күн бұрын
Robobrain is the future.
@oightKoreraAreEditable
@oightKoreraAreEditable 14 күн бұрын
yoo yo have the same name as my classmate
@chirilafilip3212
@chirilafilip3212 14 күн бұрын
@@oightKoreraAreEditable Really?
@P4ncake_.
@P4ncake_. 14 күн бұрын
arrest the neurons
@VsevolodKhusid
@VsevolodKhusid 14 күн бұрын
Cannot wait for growing brains to beat Super Mario 64
@harriehausenman8623
@harriehausenman8623 14 күн бұрын
Donkey Kong is next!
@sasukekun1997
@sasukekun1997 14 күн бұрын
This is easily my favorite Science channel. Keep up the work, this is amazing content
@scorpionlaser
@scorpionlaser 14 күн бұрын
Hey man awesome video! Noticed that your latest videos are looking better and better. Your old videos looked a bit like a slides how or a PowerPoint presentation, but these newer ones keep looking cleaner cleaner!! Fantastic work guys
@mr.richard7537
@mr.richard7537 14 күн бұрын
I was about to cut a chunk of my brain to experiment by myself BUT PART 2 CAME TO LIFE SO NOW I CAN LIVE WITH MY FULL BRAIN!!!
@timothyvanderschultzen9640
@timothyvanderschultzen9640 14 күн бұрын
DO IT ANYWAY!
@Argom42
@Argom42 14 күн бұрын
"This is your brain...and this is your brain on doom. Get the picture?"
@nullpoint3346
@nullpoint3346 9 күн бұрын
In the words of 4 year old me, "I like the spicy chemical."
@armamentarmedarm1699
@armamentarmedarm1699 11 күн бұрын
You know now you and your community need to communicate with the manufacturer of Green Dakara to make sure they either never change it, or always keep the current recipe available.
@redacted7613
@redacted7613 14 күн бұрын
“What’s my purpose?” “To play doom” “Oh my god”
@laetitiaklinkhammer192
@laetitiaklinkhammer192 14 күн бұрын
you have no idea how excited i am about this update. I've been rewatching the old video very often as a source of comfort
@Samriddha_Chatterjee
@Samriddha_Chatterjee 14 күн бұрын
From now on I shall only respond to "slightly sentient enchilada"
@doggod8138
@doggod8138 13 күн бұрын
That final array deserves to be your new channel icon, it's beautiful. (22:00)
@originsmenagerie9920
@originsmenagerie9920 12 күн бұрын
Bro’s making huge leaps in biotech just to play DOOM and I watch with great interest
@JaySlymm
@JaySlymm 14 күн бұрын
One of the few time I actually waited for a video to come out and the concept is cool af, hell yeah!
Turning Gatorade Into Meat
22:54
The Thought Emporium
Рет қаралды 871 М.
We FINALLY Proved Why Ice Is Slippery
13:43
Dr Ben Miles
Рет қаралды 485 М.
ИРИНА КАЙРАТОВНА - АЙДАХАР (БЕКА) [MV]
02:51
ГОСТ ENTERTAINMENT
Рет қаралды 5 МЛН
버블티로 체감되는 요즘 물가
00:16
진영민yeongmin
Рет қаралды 78 МЛН
Tactical Thermite Powered Hot Dog
17:08
The Thought Emporium
Рет қаралды 1,5 МЛН
This bizarre density toy just got an upgrade
13:04
Steve Mould
Рет қаралды 909 М.
The Insane Engineering of the Gameboy
17:49
Real Engineering
Рет қаралды 1,6 МЛН
The Most Confusing Part of the Power Grid
22:07
Practical Engineering
Рет қаралды 1,1 МЛН
1 vs 1000 guitar picks (5000 picks/second)
15:32
Mattias Krantz
Рет қаралды 1,2 МЛН
Negative Ion/Anti-5g Products Are Actually RADIOACTIVE
20:27
The Thought Emporium
Рет қаралды 1,9 МЛН
I made the worlds most powerful soccer shoe
21:30
I did a thing
Рет қаралды 3,1 МЛН
DIY Supermaterial Could Save You From Heatstroke: Salt based PCMs
28:11
NightHawkInLight
Рет қаралды 1,1 МЛН
I Made a Neural Network with just Redstone!
17:23
mattbatwings
Рет қаралды 660 М.
How Cathode Ray Tubes Work.
18:33
The 8-Bit Guy
Рет қаралды 221 М.
ПОКУПКА ТЕЛЕФОНА С АВИТО?🤭
1:00
Корнеич
Рет қаралды 3,2 МЛН
Ждёшь обновление IOS 18? #ios #ios18 #айоэс #apple #iphone #айфон
0:57
ИГРОВОВЫЙ НОУТ ASUS ЗА 57 тысяч
25:33
Ремонтяш
Рет қаралды 199 М.
iOS 18 vs Samsung, Xiaomi,Tecno, Android
0:54
AndroHack
Рет қаралды 93 М.
Неразрушаемый смартфон
1:00
Status
Рет қаралды 1,9 МЛН