INTRO 0:19 - Human CoVs; CoV spillover to humans 5:13 - SARS vs. COVID19 8:45 - Talk overview 1. ENTRY 10:10 - CoV structure; spike conservation 14:41 - Spike and ACE2 interaction; protease cleavage 18:30 - CoV-2 spike residues 2. REPLICATION & GENE EXPRESSION 20:08 - 2019-nCOV annotation; 14 ORFs and 27 proteins 24:05 - Ribosomal frameshifting 26:38 - Sub-genomic mRNAs with shared 5' and 3' 29:06 - Shared transcription regulatory sequences 32:13 - CoV replicase; polymerase, capping and proofreading 34:08 - Exonuclease; viruses with >20kb genome 36:14 - Loss of Exonuclease; lethal mutagenesis 38:58 - Exonuclease and methyltransferase 3. Replication-transcription complexes (RTCs) 40:54 - Interconnected double membrane vesicles 43:32 - Vesicle formation 44:32 - Proximity labelling; Characterizing RTC proximal proteome 50:20 - Accessory genes 52:01 - Assembly of nucleocapsids into virions 4. Immune interactions 53:31 - Little or no interferon in cells; delayed interferon singling 57:39 - Short lived antibody and memory B cell response OUTRO 59:38 - Key open basic science questions

I took a virology course in graduate school almost a decade ago, what an incredible distance we have come. As a microbiologist myself I really appreciate putting your lecture up.

The manner in which material is presented is exceptional. To pack such amount of information in just one lecture is evidence of years of teaching the best students.This is an excellent example of excellent teaching for all teachers in academia

Despite having no background in medicine or biology etc, I found this lecture really informative and easily understandable

Thank you for this. I am a Candian biochemist stuck in Brazil. I decided to help the local authorities out. This brings me up to speed. I shared it among the English-speaking doctors as well.

Even not being among the intended audience, i´ve been able to follow most of the lecture and have learnt quite a lot from it, and it´s really interesting, many thanks

This is one of the most amazing talk i could ever come across about the structural details of Corona virus in context of mutational potential and probable targets for drug development. I might borrow some knowledge for my own understanding and spreading of awareness in here countries bereft of educational and research facilities and governmental intent for it. Thank you for this Leviathan of a task.

Prof. Glaunsinger is a brilliant teacher. This presentation was extremely coherent and well put together. Thank you.

What a beautiful, clear minded, articulate, logical, level, educated, balanced, reasoned and highly intelligent voice. Very many thanks!! A perfect lecture.

I was looking for something like this from some time ... Thank you for providing the authentic scientific information amid this info-demic where research data is hard to find on these media platforms 👏👏👏

Many thanks for this. As a clinician, this sort of exposition is incredibly helpful in gaining a better understanding of what we are trying to manage (treat?). The link between basic science and clinical medicine is always a challenge and this sort of background knowledge is invaluable when thinking about what we are seeing in patients.

This explanation was amazing! I'm an undergraduate student, only in my 2nd semester of biochem and was able to keep with a lot of it because it was very well explained. I loved it, thank you, and also thank you to all the scientists working tirelessly to fight this.

As an undergrad biology major, I really enjoyed this in depth and straight forward overview. Thank you for making and uploading this and allowing me to apply and enhance my knowledge!

Great lecture. Very informative. I would love for Dr. Glaunsinger to give a follow-up lecture. Now that it has been almost two months with 10x more cases and more information I would love to hear her take on what's happening now.

Thank you, Dr. Glaunsinger, for that excellent introduction.

Thank you, Britt, for producing this outstanding review. I'm sending the link out to our colleagues. (Dave Sulzer, Columbia Medical School)

Studying viral biology at UG level. This is a really straight and detailed presentation. Thank you.

This is an incredibly fascinating presentation. I am so grateful for your commitment to the in depth understanding & logical explaination of this data to both the scientific & lay community. Phenomenal work!

Thank you very much for this awesome lecture! Thought I know a thing or two about gene expression, but I stand corrected. This stuff is just mind blowing.

Trust me. This whole hour of the video is worth watching. The professor details every key note about coronavirus. (I am not a virologist).

Thank you for posting this Dr. Glaunsinger. It was just what I was looking for to understand the mechanisms of the virus, and how Remdesivir might work.

It took me complete 4 hours 45 min to understand this 1 hour lecture

Thank you very much Dr. Glaunsinger for sharing your outstanding presentation! It is very detailed and understandable!

Well done. Refreshing to see some virology 101 long-form presentations to counter a lot of the misinformation out there.

Absolutely superb lecture by an expert with encyclopedic knowledge of molecular virology. A very interesting and informative video.

Thank you, Dr. Glaunsinger, for this lecture

Great discussion, I am an Immunologist. I learned a lot from listening. Thank you.

Thank you so much for this presentation. Im very far away from being the intended audience but it has been very informative in a time where we hardly get detailed information on what we are facing

Thanks for doing this. Watched full video.

Thank you very much Dr. Glaunsinger for such a great lecture

What a lecture?? Not only in context of Covid19 but also in understanding to pathophysiology of virus infections challenge in the future ahead after current drastic challenge. I recommend for incorporation of it in the pathology Curriculum word wide. NEW Normal scenario paradigm 🤗

this helped so much with my virology case study! thank you so much

An Outstanding Lecturer. Thank You Professor Britt Glaunsinger.

amazing explanations ! thank you

This is fantastic, thank you so much for sharing.

Very interesting and thorough lecture. I learned a lot even without taking cell molec!

Thank you . Was looking for this information for all long time.

Wonderful lecture, many many thanks for your efforts.

lecture seems very well put together, but it's really over my head without some background information, but I can see the work involved with putting together such an informative lecture, which is appreciated. :)

You are so well read. Thank you for sharing.

This was a really informative and well-presented lecture, thanks for sharing! All of our accumulated knowledge on coronavirus biology really stresses the importance of funding basic research on infectious diseases even when they're not yet an immediate threat.

Isn't this the deepest KZfaq video ever? Britt is the Goddess of viruses

Very informative lecture! Thank you, Dr. Glaunsinger

Outstanding lecture, Dr. Glaunsinger!

Thanks for this lecture. Very thorough! Wondering if the more conserved fusion domain of the S-gp, or the polybasic linker would make a good target for mAb therapeutics?

very informative and sufficient in depht current knowledge, helped me a lot. Thank you so much Britt :)

Thank you so much! This is very informative yet easy to understand! Nice presentation!

This wonderful lecture is well above my level of comprehension. It would be great if Dr. Glaunsinger could present a youtube lecture addressed to the lay person showing how SARS CoV-2 could be defeated.

Was boring in the beginning, and then it turned insightful, educational (not only regarding Coronaviruses), interesting and some other adjectives too. Glad I found such a high level source on KZfaq.

Thank you for the amazing lecture. It was easy enough to follow despite my limited comprehension when it comes to sciency stuff.

the mechanisms by which virus genomes rely on their host and how the transcription machinery is modified is really fascinating.. especially the discontinuous transcription and the Exon protein... . I enjoyed this very much, thank you.

Absolutely fabulous you presented the science extremely well, until we understand fully the biochemistry we cannot create a cure. As a former biochemist and now a doctor. I applaud you on a fabulous presentation.

Thank you Dr. Glaunsinger.

Thanks for this video. I sent it to many of my colleagues.

Cool.. Free Lecture. High Quality. God Bless You

@51:25 Please elaborate more on the association of INF and role of IFN inhibitors on viral accessory proteins.

Very nice compilation of current knowledge of SARS-COV-2 molecular virology. As a retired biomedical scientist, makes me want to put on a white coat and get back at the bench.

fantastic video. great visual aids. also link to more lay info. superb

Excellent...quick updates. thank you

Excellent lecture having deep understanding of molecular virology

pure gold, thank you!

Awesome lecture! Could you please upload more about Coronavirus? I would love to learn more about it in a molecular level. Thank you so much!

Excellent presentation, thanks for posting.

really thank you for sharing all this knowledge!!!

Nice lecture. Steady flow of information. Well organized. Thanks.

Excellent presentation. Very clear and precise speaking style. Good job!

Thank you! Dr. Glaunsinger, this has been an incredible lecture! Easy to follow and understand. Equal parts educational, and terrifying. But also, calming to known this virus better :D

For an hour of lecture, I have learnt a lot of coronaviruses and it is very useful. Thank you so much, Professor.

As a layman, I want to thank you for a very interesting talk on the virus' mechanisms. I have probably 20% or less of the prerequisite knowledge to fully understand the contents, so, though I undoubtedly missed a number of nuances, I feel I could explain the top tier of info you provided. Thank you for making an accessible talk without paring it back too far. I'm stunned at the tactics of the virus at each stage of it's cycle, and,frankly, equally stunned that we can collectively discover and understand them. Good job to all involved. Thank you, and keep up the good work!

this is amazing work! Thank you !

Thank you for this informative lecture 🙏🏻🌷

Dear Britt, what is exact role of Furin in the process of Covid19's lifecycle/entry into human cells? Please shed some light on this aspect.

really excellent ... easy to digest and clear ... big thanks!

Thank you so much for that lecture Dr Glausinger and IGI team. I was wondering if we are going to be able to figure out with certitud about the intermidiary species that hosted CoV-2 before it jumped into the human species? Is it a matter of more data/research needed or is it like a piece of the puzzle we will never know for sure?

Great lecture, thank you!

Awesome lecture, thanks a lot!

thank you for the great, detailed video

Great explanation....stay safe everybody 💫🙂

Informative lecture... thank you 🤗

Thanks for sharing such an interesting lecture.

👍🏼 thank you. For me it was very informative on genome structure and details on transcription and replication. Thanks again.

Thanks a lot. I 've learned a lot from that lecture.

Incredibly lecture, thanks for it

Very Brilliant of your Discussion Professor. I would be interested to know, what are the underlying aspects of such tremendous diversity in viral strains.? can we attribute the phenomenons of Epitope transformation and genetic variability due to absence of proof reading mechanism.? or some species mediated interactions in response to which viral pathogenesis has become stronger.? and do we have some counter act approaches to fight these virruses.? I would like to have your expertise in this regard. Thank you.

This was beyond my Biotech Cert 1 knowledge but this was extremely insightful. As a RN this still interests me a bunch.

Thank you for being a teacher.

A great lecture!! Quiet complicated!!

unnerving to hear such a knowledgeable calming voice describe this horrific time. our current global sadness, pain, and death deserves all the transparency labs and specialists can honestly pour out of their brain.

Prof. G. Thank you for the wonderful, insightful presentation. I have to point out one thing. ACE 2 is an enzyme and has substrates (angiotensin 1 and 2). There is no ACE 2 receptor. SARS Covid 2 uses ACE 2 as a "receptor" to enter the cell. So ACE2 is a "receptor" for SARS Covid 2 and one may call ACE2 an SARS Covid 2 receptor. It actually functions as an acceptor. Pharmacologically, a receptor, upon being bound by a ligand, should trigger a cascade of biochemical/physiological reactions. Virologists use the term receptor differently.

best video seen so far on SARS-CoV-19!

You are a Heroine! You deserve recognition a Medal of Honor!

great video, thanks

That's an awesome lecture... Thanks a ton!

Wonderful Lecture ! Finally something reliable. Thank you !! from Morocco lots of disinformation here.

@ 14:15 cannot we strick or restrict the action of conserved fusion part of spikes.(shown in purple).as that is non variable and can be dealt by pharmaceutical interference as it has very vital role in entering the cell structure.. just suggesting and I am total layman for genomic

Does the ACE2 receptor have any enzyme activity during its interaction with the virus or is it just structural?

very easy to understand and lots covered

Very informative lecture. Thankyou.

very nice, thanks for the explanation. Can't we get the PPT ?

Great lecture, many thanks!