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Determination of Starch Content | A Complete Procedure | Acid Hydrolysis Method

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MicroChem's Experiments

MicroChem's Experiments

Күн бұрын

Starch constitutes the major component of the polysaccharides in most foods and consists mainly of amylose and amylopectin. The test sample is dispersed in water and heated in acidic solution to hydrolyse the starch, releasing the sugars. The resulting sugars are then determined by titration with Fehling solution according to the method of Lane-Eynon. The sugar content is further converted into Starch content of that sample. This video represents a detailed procedure for the determination of total starch content of a food sample by Acid Hydrolysis Method.
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#starch
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#totalstarch
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Пікірлер: 38
@JoshiNirav30
@JoshiNirav30 Жыл бұрын
Appreciate the amount of work and editing on this video, please make a video on "Total Dietary Fibre by Enzymatric Gravimetric Method"
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Noted. Thanks
@hannagraceguirigay1720
@hannagraceguirigay1720 Жыл бұрын
Can you do a video explaining the formula used? Where does the 0.9 come from? 10:09
@yadavhealthcom
@yadavhealthcom Жыл бұрын
Thanks 😊
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Welcome
@vladimirosvasileiadis7742
@vladimirosvasileiadis7742 Жыл бұрын
Hello i really need your help. I want to make an acid hydrolysis of proteins. Can you please tell me a method to do this? I know the most common is with hydrochloric acid but i want to learn the method. Thanks
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Digest 2g of your sample with 20ml concentrated Sulfuric Acid
@nurulnadhirah64
@nurulnadhirah64 Жыл бұрын
I want to ask, for the solution that needs to be tested for the total sugar method, is it the solution needs to do it immediately, or can wait for a few days?
@adepojulanre5234
@adepojulanre5234 10 ай бұрын
Why phenolphthalein indicator not added?
@FST307
@FST307 Жыл бұрын
share vedion on colouring matter determination in ready yo eat food
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Noted
@nurulnadhirah64
@nurulnadhirah64 Жыл бұрын
so to get the starch content, just only times with 0.9? why 0.9? may i know
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
The reference method said so.
@narendarpratapyadav9908
@narendarpratapyadav9908 Жыл бұрын
@@MicroChemsExperiments .92
@nurulnadhirah64
@nurulnadhirah64 Жыл бұрын
May i know what references do you use to get 0.9?
@byrnardsalavedra5969
@byrnardsalavedra5969 Жыл бұрын
hi sir/maam, can we use any type of condenser for this analysis? what is most preferred?
@alyciaani
@alyciaani Жыл бұрын
what gas would be released during the acid hydrolysis please
@akanmudamilare7235
@akanmudamilare7235 22 күн бұрын
Can this method be used for starch content evaluation in flours?
@MicroChemsExperiments
@MicroChemsExperiments 21 күн бұрын
Yes
@nurulnadhirah64
@nurulnadhirah64 Жыл бұрын
do you have the method to determine starch content by using lane and eynon method?
@nonatarbah2951
@nonatarbah2951 Жыл бұрын
Could you answer me How do you make acides? how calculate them? Is there a way or low for calculate base and acid which use in determine at lab?
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Please study chemistry more to do these. Take help from your school teachers.
@therese6051
@therese6051 Жыл бұрын
Hello! What specific type of reflux condenser is used in this process? Can we use the liebig type of reflux condenser? Or does it have to be the same design and type?
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Any reflux condenser what will facilitate the condensation is applicable
@nainanizami7924
@nainanizami7924 Жыл бұрын
can we use this method for determining starch percentage in turmeric?
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Yes. You can.
@ramisfadhil7384
@ramisfadhil7384 11 ай бұрын
Total sugar 47.5 % how it come? And 0.9 😢
@nainanizami7924
@nainanizami7924 Жыл бұрын
is this procedure taken from AOAC ?
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
No. It is laboratory validated method based on Lane Eynon method
@narendarpratapyadav9908
@narendarpratapyadav9908 Жыл бұрын
sir starch industry ke bhi video banaye
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Noted
@danielpecori6532
@danielpecori6532 Жыл бұрын
Hello, can I use this method for determining starch porcentage in corn grains?
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Yes
@nonatarbah2951
@nonatarbah2951 Жыл бұрын
What the meaning 50% Naoh
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Please study chemistry more to do these. Take help from your school teachers.
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