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Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry - a 2 minute Preview of the Experimental Protocol
Mirella Vivoli, Halina R. Novak, Jennifer A. Littlechild, Nicholas J. Harmer
University of Exeter, Department of Biosciences;
Differential scanning fluorimetry is a widely used method for screening libraries of small molecules for interactions with proteins. Here, we present a straightforward method to extend these analyses to provide an estimate of the dissociation constant between a small molecule and its protein partner.
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