Direct ELISA (FL-Immuno/70)

Рет қаралды 186,877

6 жыл бұрын

In this video lecture we will study...
Direct ELISA
Difference between direct and indirect ELISA
Application of DIrect ELISA

Пікірлер: 59

@annettematadi5513 4 жыл бұрын

Loving this. Well explained!

@emanothman9665 6 жыл бұрын

Itis exam night and those vid series really help

@FrankLectures 6 жыл бұрын

Glad to know this. Thank you for your feedback.

@jasonclintespinoza1343 2 жыл бұрын

so much better thanks!

@swaroopsinghjakhar3971 6 жыл бұрын

Awesome presentation about immunology reaction....

@FrankLectures 6 жыл бұрын

Thanks

@ibrahimlawan8056 6 жыл бұрын

Simple and clear explanation thank you

@FrankLectures 6 жыл бұрын

Thank you.

@parinaazfatimah1710 5 жыл бұрын

Getting addicted to ur videos .... gr8 job team

@FrankLectures 5 жыл бұрын

Thanks

@nagwaabou-soliman2621 3 жыл бұрын

thanks so much for this video

@deryaprl9735 3 жыл бұрын

Great!

@zoief4088 6 жыл бұрын

Very nice video. Thanks.

@FrankLectures 6 жыл бұрын

+Zoie F thank you for your feedback

@subscriberwithoutanyvide-rq5ym 5 жыл бұрын

Thank you for making it easy

@FrankLectures 5 жыл бұрын

Thank you for your feedback.

@samialhajaj8477 2 жыл бұрын

Thank you so much

@swaroopsinghjakhar3971 6 жыл бұрын

The Elisa test requires A) know Antibody B) know Antigen C) Patient Antibody d) substrate Please answer with reference One of Rajasthan health university of science is claiming it's answer B while as per your video it's answer is A

@FrankLectures 6 жыл бұрын

I find this question incomplete. This is because ELISA test can be used to detect both antigens and antibodies. In case, we want to detect the presence of specific antigens in the patient's serum, we should have known antibodies for this antigen (DIRECT and SANDWICH ELISA). And in case, we want to detect the presence of specific antibodies in the patient's serum, we should have known antigen (INDIRECT ELISA). Now, because most common application of ELISA test which we study is the HIV screening test. So, according to that, answer should be B. Because in HIV screening test, we detect antibodies (INDIRECT ELISA). Known antigens are immobilized in the wells.

@swaroopsinghjakhar3971 6 жыл бұрын

Please provide me standard reference.... Because I was participated in in exam and examiner authority declaired answer is know Antigen

@lethabomoses7654 3 жыл бұрын

Nice video. Um my question is how do we get to immobilize the antigens since they are our analyte provided that we are not sure if they are present or not in the patient's sample?

@sulaimanolaide2036 Жыл бұрын

Yeah! How do we immobilised the Ag? I hope we are not using Ab to immobilise it or else what will differentiate the Direct And Sandwich ELISA. Thanks

@kelvinkisabuli7808 3 жыл бұрын

THANKS

@donajor8 5 жыл бұрын

By Elisa there are many positive igg as ebv hhv6 cmv But with negative igm. And negative pcr? What do this mean with clinical symptoms?

@FrankLectures 5 жыл бұрын

Please elaborate your question. I am unable to understand what you are asking?

@donajor8 5 жыл бұрын

On the Elisa test for ebv, cmv, hv6 the igg are positive and the igm are normal. Also the pcr for these viruses are normal. And the person is have symptoms. What to make out of this situation.

@FrankLectures 5 жыл бұрын

Sorry buddy ! Your question is out of the scope of the syllabus I have covered. Need to study more for this.

@donajor8 5 жыл бұрын

Thanks still

@mohammedelshaer6884 6 жыл бұрын

does this method used for quantitative assay or just qualitative as shown ?

@mohammedelshaer6884 6 жыл бұрын

and thank you for the series.

@FrankLectures 6 жыл бұрын

ELISA is used both as a quantitative and qualitative assay. But Direct ELISA is not the preferred method. Indirect and sandwich ELISA are preferred.

@FrankLectures 6 жыл бұрын

You're Welcome.

@mohammedelshaer6884 6 жыл бұрын

how it can be used in quantitative assay if we lose some of serum (antibod or antigen) when rinsing ?

@FrankLectures 6 жыл бұрын

I don't have detailed information on this. More deep study about ELISA protocol is needed for this. Sorry, unable to answer right now.

@asmaabbasi3277 Жыл бұрын

in direct ELISA we always measure antigen..??

@warood9690 5 жыл бұрын

Thanks

@FrankLectures 5 жыл бұрын

You're Welcome

@alokkumarsrivastava2959 5 жыл бұрын

What is substrate in this?

@inshaishrat4371 3 жыл бұрын

It is the molecule or compound specific to the enzyme used to conjugate the antibody. It (complex of enz and substrate) is chosen in such a way that it gives colour after substrate is added to the mixture/ well containing the enzyme conjugated-Ab. Some examples are HRP enzyme with TMB substrate to give green colour.