DNA microinjection | Embryonic stem cell mediated gene transfer | Gene transfer techniques

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Bio science

Bio science

4 жыл бұрын

#genetransfer
DNA microinjection | Embryonic stem cell mediated gene transfer | Gene transfer techniques
DNA microinjection is the dominating technique leading to random integration of a transgene via the introduction of DNA into the pronucleus of a developing zygote. Following fertilization of a mouse egg, the male and female pronuclei remain separated for a few hours before they fuse to make the zygotic nucleus, thus allowing for microinjection of the desired genes into the larger male pronucleus. Eggs that can survive the injections are transferred into the oviducts of a foster mother i.e., pseudopregnant female mice for the generation of Founder mouse, from which permanent transgenic lines can be established. The presence of transgenes in the offsprings can be identified by PCR analysis or Southern blot hybridization.
For embryonic stem cell mediated gene transfer, skip this video directly to 5.45
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Electroporation
Gene transfer techniques
Dna
Rna
Transcription
Translation
Proteins
Animal biotechnology
Animal transgenesis
Alampally phanindra
Phanindra gupta
molecular biology
dna microinjection method
microinjection method of gene transfer in plants
dna microinjection transgenic animals
microinjection gene transfer method
embryonic stem cell mediated gene transfer
microinjection gene transfer method animation

Пікірлер: 72
@Akashkumar-jo6mp
@Akashkumar-jo6mp 4 жыл бұрын
Beautifully explained..! Thanks hope your channel grow well ..
@bioscience6853
@bioscience6853 4 жыл бұрын
My pleasure✨
@madihaali7173
@madihaali7173 Жыл бұрын
Amazing explanation
@deepikasripally8839
@deepikasripally8839 Жыл бұрын
Thank you ☺️
@bandarimahi23
@bandarimahi23 9 ай бұрын
TQ soo much for your clear explanation ❤ sir
@sudhakerketta6578
@sudhakerketta6578 2 жыл бұрын
My seminar topic is "gene transfer to animal cells" I was so confused about methods... But after watching this... I feel relaxed lil bit🙂 thank you sir 🙏 I hope I can...
@bioscience6853
@bioscience6853 2 жыл бұрын
Thank you
@sunkisatheesh1464
@sunkisatheesh1464 2 жыл бұрын
Super explanation sir. Thanq.
@bioscience6853
@bioscience6853 2 жыл бұрын
My pleasure
@galmaankhan8338
@galmaankhan8338 2 жыл бұрын
I'm a little bit confused. In embryonic stem cell mediated gene transfer method, if the the target genes are incorporated into the ESCs and then added to blastocyst and finally when it is implanted into the host, why do the host has to undergo fertilization? Because after fertilization, the blastocyst will be formed.
@belysultana8177
@belysultana8177 Жыл бұрын
Useful vedio. That helps to understan the topic clearly
@bioscience6853
@bioscience6853 Жыл бұрын
My pleasure
@abdum6977
@abdum6977 3 жыл бұрын
Very nice and simplest explanation 👍👍
@bioscience6853
@bioscience6853 3 жыл бұрын
Thanks
@akramrahi3529
@akramrahi3529 2 жыл бұрын
Thank u sir I watch the full video that very clearly I appreciate u sir
@bioscience6853
@bioscience6853 2 жыл бұрын
Thank you
@naveenshanmugam9848
@naveenshanmugam9848 2 жыл бұрын
Thank you brother you cleared all my doubts and now i am ready to take the seminar 🥰🥰🥰 keep posting more videos ✌️✌️✌️
@bioscience6853
@bioscience6853 2 жыл бұрын
Sure
@act_pvt
@act_pvt 2 жыл бұрын
Thank you so much
@bioscience6853
@bioscience6853 2 жыл бұрын
My pleasure
@anshikaapatkare
@anshikaapatkare 2 жыл бұрын
Sir can we introduce more than one target gene in the ES cells
@ssjewellers4755
@ssjewellers4755 4 жыл бұрын
Thank u sir good lecture
@bioscience6853
@bioscience6853 4 жыл бұрын
My pleasure✨
@shivmunisigh9997
@shivmunisigh9997 3 жыл бұрын
Thank you sir
@bioscience6853
@bioscience6853 3 жыл бұрын
❤☺❤
@syedsafeenanisar7290
@syedsafeenanisar7290 3 жыл бұрын
U are awesome bro. Thnks
@bioscience6853
@bioscience6853 3 жыл бұрын
☺☺
@manikgrover9107
@manikgrover9107 3 жыл бұрын
sir plz help me ...about retroviral transfection plz send me link of that vedio of urs
@timothy8048
@timothy8048 Жыл бұрын
Thanks yrr
@PradeepSharma-ii6jr
@PradeepSharma-ii6jr 2 жыл бұрын
When foreign DNA is transferred to the host cell, how does the foreign DNA bind to the nuclear DNA of the host cell?
@MoviesNepal23
@MoviesNepal23 2 жыл бұрын
sir please kindly makes the video about mitochondria and chloroplast gene transformer
@enjoywithindrajeet891
@enjoywithindrajeet891 3 жыл бұрын
Sir aap video rcording kis chij se karate hai bataye please
@hirendabhi7675
@hirendabhi7675 4 жыл бұрын
WHICH FACTOR RESPONSIBLE FOR THE NEXT GENERATION WILL TRANSFER INTO GENE TARGETED MICE OR NORMAL MICE.
@gayatrihaldar5570
@gayatrihaldar5570 2 жыл бұрын
Thnku so much
@bioscience6853
@bioscience6853 2 жыл бұрын
My pleasure
@areebamubeenashraf9571
@areebamubeenashraf9571 3 жыл бұрын
In microinjection technique, can we use sperm cell as a host.??After which we fertilize egg cell and the sperm which have foreign DNA. So kindly give me ans...
@TheMinha
@TheMinha 9 ай бұрын
Fan from Kashmir
@person.2563
@person.2563 2 жыл бұрын
You make it clear and easy for me🤝 Thank u🤍🤍🤍
@bioscience6853
@bioscience6853 2 жыл бұрын
My pleasure
@ekhushimanzoor6186
@ekhushimanzoor6186 3 жыл бұрын
Nice
@bioscience6853
@bioscience6853 3 жыл бұрын
Thanks
@nothingggg656
@nothingggg656 11 ай бұрын
Sirr which vector is used for microinjection plzzz reply
@shazialone7545
@shazialone7545 3 жыл бұрын
Sir where is retroviral gene transfer method topic?
@Risky_Rambles
@Risky_Rambles 3 жыл бұрын
Thnks
@bioscience6853
@bioscience6853 3 жыл бұрын
My pleasure
@AliKhan-ns2dp
@AliKhan-ns2dp 3 жыл бұрын
Good but your explanation method is very fast so sorry did not understand on single word
@saroshsaroshali9654
@saroshsaroshali9654 4 жыл бұрын
Sir explain in hind..and also explain other methods of transformation
@tanmoydas6861
@tanmoydas6861 3 жыл бұрын
Can this process used human?
@gudipatisridivya7283
@gudipatisridivya7283 3 жыл бұрын
sir please help me with retroviral gene transfer
@bioscience6853
@bioscience6853 3 жыл бұрын
Sure
@omerabdullah8556
@omerabdullah8556 Жыл бұрын
kal exma hai isliye yaha aaa k dekhte🤣
@dimplethakur6447
@dimplethakur6447 4 жыл бұрын
Sir what is difference between electroporation and microinjection
@ritwikbasak4960
@ritwikbasak4960 4 жыл бұрын
Electrical voltage is used in electroporation for creating pores around cell membrane. And in microinjection you can see it in the video
@kirtisingh46
@kirtisingh46 4 жыл бұрын
Sir ak video hinde me bhi bta de
@Krishna9292p
@Krishna9292p 4 ай бұрын
sir hindi me explain Karo 😢 nots mast bhanaya hai ❤
@vaishnavitomar6001
@vaishnavitomar6001 2 жыл бұрын
Bhai thoda sa aaram se bolo itni jldi kha Jana hai kuch samajh bhi nhi aarha baki Nice explanation 👍
@teenasharma7323
@teenasharma7323 3 жыл бұрын
Aap thoda dhere dherre samjhaya karo well done 👍
@bioscience6853
@bioscience6853 3 жыл бұрын
Thanks
@akramrahi3529
@akramrahi3529 2 жыл бұрын
Next topic transgenic anamils please sir tell me
@bioscience6853
@bioscience6853 2 жыл бұрын
Sure
@saroshsaroshali9654
@saroshsaroshali9654 4 жыл бұрын
Urdu mein ya hindi mein
@mittapelliramanna1401
@mittapelliramanna1401 3 жыл бұрын
Sir thelugulo cheppandi
@saroshsaroshali9654
@saroshsaroshali9654 4 жыл бұрын
Urdu mein explain krein sir
@bioscience6853
@bioscience6853 4 жыл бұрын
I don't know urdu
@swapnabehera3451
@swapnabehera3451 3 жыл бұрын
Ur words are very speed. Little difficult to understand 😐
@mrwrong2893
@mrwrong2893 3 жыл бұрын
You are going to....
@SonaliRajput2020
@SonaliRajput2020 2 жыл бұрын
PK
@Sane_gupta
@Sane_gupta Жыл бұрын
Go to 5:07 for embryonic stem cells
@m.s.choudhary4115
@m.s.choudhary4115 3 жыл бұрын
Sir please explain in hindi
@bioscience6853
@bioscience6853 3 жыл бұрын
If I explain hindi... South indians can't understand... Hope u understand 🙂
@m.s.choudhary4115
@m.s.choudhary4115 3 жыл бұрын
@@bioscience6853 but sir You can make this video in hindi or not ? We are so many students of rajasthan have subject biotechnology and we are getting right teacher I feel you are a best teacher so please help us We will be so much thankful of you
@AmruMagdy
@AmruMagdy 3 ай бұрын
Amazing explanation
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