Don't Buy A Flow Hood - How to Actually Use a Still Air Box

Рет қаралды 51,721

Күн бұрын

linktr.ee/mycodogs
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You don't need to spend a ton of money on expensive lab equipment. Still air boxes, if used correctly, are perfectly suited for the home cultivator.
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00:00 intro
02:48 prep
03:27 choosing your SAB
04:47 tools
07:33 general techniques
10:26 agar
13:12 grain
15:11 liquid culture
16:45 conclusion
================Supplies I use=================
INDUCTION STERILIZER PARTS:
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Пікірлер: 189

@user-pu6yw6po4e 9 ай бұрын

Very interested in how you built/installed that induction coil. Would love to see a video on that. Great work as always!

@joshuarohrer3261 7 ай бұрын

I agree

@neveride 6 ай бұрын

kzfaq.info/get/bejne/d7V9l6lytLrGnXU.html

@KOKO-uu7yd 6 ай бұрын

YESYESYES

@LoudNTechie 10 ай бұрын

I really hope to see that induction sterilizer tutorial someday! It's pretty awesome!

@edwardgrand 11 ай бұрын

Very nice video. Good to see someone using an SAB correctly and smoothly :)

@MycoDogs 11 ай бұрын

Thanks Ed I'm glad u enjoyed it

@MikeKincaid79 8 ай бұрын

I'd love to see that induction sterilizer video you mentioned. Got the parts loaded into my Amazon basket, just need the guidance on how it all goes together. Thanks for your work!

@timgrot1385 10 ай бұрын

Very concise and informative content to a newbie trying to learn as much about this as I can. Between you, 90 second mycology, Bass drop keys and willy mico, I'll have a first full flush in no time! Much love to all you guys inspiring us just getting started!

@chanteb3972 6 ай бұрын

PGT is another great KZfaqr

@prometheus1592 8 ай бұрын

that damn coil worked like MAGIC

@FullDottle 10 ай бұрын

Bro, your induction coil sterilization set up is absolutely awesome! I just got my supplies in today to make one! Thank you for sharing and linking the supplies!

@Scarab80 11 ай бұрын

I've had massive success with still air boxes. Love your vids!! ❤️

@deadpoolest899 11 ай бұрын

If you have an old coffee can or something or the sort/size, you can set it on the burner on your stove for a bit to heat up and then melt the hand/arm holes into the SAB. Wont have to worry about cracking the tub either while cutting/drilling!

@Animal-Reaction-Clips 11 ай бұрын

I did that with dog food lol 22 years ago 😅

@michaellotto6164 11 ай бұрын

This comment is underated

@CrabbyPattydelight 2 ай бұрын

And I seen how they put the gorilla tape on each side of the hole since it's a big coffee size hole and cut an x in the tape sticky to sticky over the hole like a skirt, maybe not necessary but seems like a good design

@koukouzee2923 7 ай бұрын

Man i just love how you explain stuff I can watch this all day

@user-ym5kf4gq5c 8 ай бұрын

Thank you my good sir for sharing your hard earned experience with us freely! Boundless Love & Light to you❤

@alen_ELMLB 8 ай бұрын

Great video Damn that induction sterilizer looks awesome

@dennisanderson8337 4 ай бұрын

This is very well thought out and described. Thanks!

@nullobject7966 7 ай бұрын

Looking to get a SAB for tissue culture. I found this video super helpful. I'm very interested in the induction sterilizer. It's absolutely brilliant.

@carmenreyes4669 9 ай бұрын

I'm a newbie. Thank you for all the knowledge.

@DreamInVader360 8 ай бұрын

I would love to see a video on how to build the induction sterilizer. Great video thank you 😊

@shashipatel6885 9 ай бұрын

Definitely interested in how to build that induction coil

@mikeos 10 ай бұрын

Really helpful video, thanks! Love the background music too! Subbed

@JimmyGreen-wx6gt 7 ай бұрын

Great video man, lots of good tips.

@dingdang3845 11 ай бұрын

Right on! 🤟🏻 I appreciate content that I learn from

@Katimashi 11 ай бұрын

Yo dude! Just gotta say the advise and information you gave me when I first started has been so helpful!. I appreciate you! Borf!

@hobbes301 6 ай бұрын

This is a fantastic video, your technique is masterful

@dustinewing6767 11 ай бұрын

Man frfr, I have been a part of the community and a hobbyist mycologist for some years. But this is the thing is I have a Flow hood, also a FFU, and my old friend my Still Air Box. My SAB has been old faithful. Heck I have the FH, and my FFU. And still this I use my SAB for certain things, small jobs. Lol PLUS I HAVE HER ROCK SLAPS EVERYWHERE. I GOT MY FH COVERED IN THEM TO!!!! Man they look great. But my FFU is clean no slaps on her. But I agree 100% with everything you covered. I do certain things like you, but some other I do different. Plus you showed me some things to try out myself. Keep up the great work.

@Mycologypadawan 11 ай бұрын

Yo im trying to get to know as mush as possible before beginning to even try to grow my own mushies and damn youre videos are amazing keep it up!!! i learned so much already

@Fer_Nav520 11 ай бұрын

you should get more views, your content is amazing!❤

@Landonx6 11 ай бұрын

fr so much awesome info

@calebsmith5764 9 ай бұрын

Id love to see the setup on that induction sterilizer the only diy videos I can find don’t use a pedal and I want to incorporate it for sure.

@ELD4R0 11 ай бұрын

video tutorial for the induction sterilizer, I love it 🙏🙌

@Mr.St3wi3 10 ай бұрын

I'm currently building the GordoTek "flowhood" My place is pretty humid and dusty. Great video btw

@demetriahardy6561 8 ай бұрын

Love the patience and motivation you put out to us jumping in the community. Thank you! 🎃💪🏾

@Mohammadparvazi 11 ай бұрын

I'm gonna make a check list using what you said it's so perfect thanks

@SL-yy1gw 7 ай бұрын

Would definitely love to see a assemble video on that induction coil fr

@fredmeds 10 ай бұрын

Well done! ✌

@Quezecotl2012 4 күн бұрын

Really excellent info done well b

@Lemonz1989 8 ай бұрын

I don’t use alcohol inside the SAB anymore, because I have a strong UV-C light inside. I turn it on for 15 minutes to sterilize the inside of the SAB before I start working, and turn it off when I have to sit next to it, or else I would go blind, lol. Then I use alcohol on everything that enters the SAB before I put it through the arm holes. Never had anything contaminated since I started doing it this way. I even let Petri dishes harden/cool without the lids on inside the box, so I avoid condensation, and just have the UV-C light turned on the entire time the lids are off. 😊

@nisar8009 8 ай бұрын

I do as well, but you have to are sure nothing living is in the room when using the light. And I still use alcohol as a backup. I’ve never experienced contamination.

@andersschroder2483 5 ай бұрын

Can you point me to the lamp youre using

@robtron2 7 ай бұрын

This is great, thank you. Subscribed.

@huntintrailmetals9343 2 ай бұрын

Awesome video thanks my bruda!

@mattemmerson5877 17 сағат бұрын

Flipping the lid over...so simple and easy, sure wish I'd seen this video when I started. Drilling holes in lids is a pain. Where do you get rye grain berries and are they worth the expense? I've had pretty good success with popcorn.

@Navagenetics 10 ай бұрын

I love your vids bro . Remember, “ignorance is bliss , knowledge is power “ - nava

@StickyDankFingers 8 ай бұрын

Totally interested in DIY induction coil sterilizer video. Thanking you in advance :)

@methylene5 6 ай бұрын

Quite a few explosions have occurred when flame sterilising needles inside still air boxes. If the alcohol vapour is in the right proportions with the air (upper/lower explosion limit), then it will ignite/explode. So, be careful and only use the minimum amount of alcohol when wiping down surfaces, and no open flames.

@davidlee50 2 ай бұрын

FYI, Those Sterlite Containers have 2 Holes at the lid level near the handles and make great connections to air tubing and air stone in water vases.

@wildfire8341 10 ай бұрын

Thanks for sharing .. I like your style 😎

@WavyDavis803 11 ай бұрын

Loads more actually good advice you should have more views than my rookie mycologist

@ppr3539 4 ай бұрын

Appreciate the video! Compact and full of information! Never the less, because we are in the topic sterile workflow, does somebody have any tips on putting the grainspawn into bulk in a monotub in the most steril way possible. Its my first time soon and i am a bit paranoid about Contamination. Obviously i dont have a flow tube neither a Still Air Box big enough to but my Monotub in.

@brettwood6721 11 ай бұрын

Thx Bloke, much appreciated. Best thing i did , was make a SAB, before i even colonized my 1st rice bag :)..Makes good sense..makes good practice. Also, tyvm for the diff tech tips, agar plates etc. Some great ideas/best practice strategies. Cya down in the Lab..BLACK TAR GANG! 😎💨💨🖤💯🤘

@shaunm1983 10 ай бұрын

Great video thanks

@legendno1 7 ай бұрын

i keep my plates upside down as well while i transfer.. got the idea from you

@fefo2109 11 ай бұрын

1:50 "70 bucks" said Madden xD hahaha i reconize that microscope! Nice to see you! Peace!

@Gabi-he6rw 3 ай бұрын

Thank you for infos. Do you say that the liquid culture jar doesnt need a filter to breath? This means that i can make a needle biopsy, suck some sterilized liquid and air into it and mycelium can grow inside the syringe?

@altaccount2179 9 ай бұрын

Very informative 👏 i will view more of your content

@aarongentile7232 6 ай бұрын

Great video!

@marinamay7024 8 ай бұрын

I’m very interested in how to build the induction coil into the SAB, appreciate if you can make a video about it! Mush Love ❤️

@KingSezam 9 ай бұрын

Please do a tutorial on the sterilizer:)

@modernmountaineer 9 ай бұрын

Ive been having very good results working in open air. Just shutting the bathroom door with a towel at bottom and turning on a hot shower till steam covers everything and spraying the air around and over me with alcohol and quickly bang it out. Im bout to make a diy flowhood i think just to reduce the occasional failure. The most important parts imo is making sure your grains are fully sterelized, fully colonized, and your substrate isnt to wet.👍

@MycoDogs 9 ай бұрын

yea ive done transfers just on my desk and they work if ur careful

@craftycaster7043 5 ай бұрын

Adding a bleach towel to the bottem of the sab works wonders

@llllllllllilillii 6 ай бұрын

excellent info

@TheTubejunky Ай бұрын

Used a industrial led work light and it is connected to a remote switch so I can "no touch" operate it.

@DangerRussDayZ6533 6 ай бұрын

Working in front of a ffu is such a QOL improvement. I hated working in a SAB

@benjammin2L8 10 ай бұрын

6:40 sounds like you discovered a flash sterilization method 😂

@j1567 9 ай бұрын

Dude. I love you ❤️

@MrDaMassi 6 ай бұрын

Hi can you show how to build the induction coil? Very cool to see !!!

@MycoDogs 6 ай бұрын

I have a video on it

@philipholman9898 3 ай бұрын

Biggest problem for me is reading to be graduations on the syringe when using a still air box

@madmaxofspokane1691 10 ай бұрын

What are the dimensions of the SAB? The tubs that you listed are definitely not big enough. I just don't want to waste any money getting a box that's too small and you have no link for one.

@oliverbenji1807 8 ай бұрын

I spray a lot 70% iso in sab when i using it so the voltage heating still safe in that case ?

@TheTubejunky Ай бұрын

Those induction coils are cheap if you know how to make one. Use a zvs and a coil with center tap.

@manicfestdestinyyall1727 9 ай бұрын

Off topic of the video, but what type of lighting and how much for the all in one grow bags? Trying to remain reasonable on price as I am new to this. Thanks for any help and really enjoying the channel. Scratch that. Just went to the discord channel and that was the most recent post.🤣

@SixTough 4 ай бұрын

I mean it doesn't look like anything is clean in this video. Yet I trust that it is

@kenoz765 10 ай бұрын

the myco gods sent us a genius furry to teach us his secrets

@PURGE2021 8 ай бұрын

Look for the video on the induction sterilizer. Id like to know how to make it. Please make a video or share the link where i can find it. Mush love

@WavyDavis803 11 ай бұрын

Thanks!

@MycoDogs 11 ай бұрын

Thanks for the super man

@elizabethr2908 6 ай бұрын

Can a grow tent be used for doing lab work if the exhaust fan is blowing clean air in through the HEPA filter?

@MycoDogs 6 ай бұрын

It might be better to seal the tent, run the filter inside it for like 30 min then shut it off and wait til the clean air is still in there

@mekolines8781 6 ай бұрын

Hey, i appericiate your Video, thanks for all the practical information. I got one question. You said you wouldnt recommend growing mushrooms for a profit. Can you explain why ? You can start with a relatively low budget and if you work clean you get constant results. Im thinking about delivering my mushrooms fresh to the local gastronomy of my village. Why cant you recommend ? Or cant you just recommend it for the us ?

@MycoDogs 6 ай бұрын

i dont recommend it because in the majority of locations they will lock you in a cage if they find out

@shaqx7039 7 ай бұрын

Are you able to replace filters in laminar?

@ochretide 10 ай бұрын

Diy induction sterilizer! 😮

@TheTubejunky Ай бұрын

I have a 1000watt induction heater it only cost about 48$ years ago. So i know there are cheap ones.

@courtneypannell563 2 ай бұрын

Too cut the holes use a razor blade and vice grips and a torch.. clamp the blade at and angle away from you hest the blade till red hot and cut like butter

@raytry69 4 ай бұрын

7:24 It is able to start the alcohol to burning isnt't it? What is visibly glowing hot that is well over 500C degree.

@concernedcitizen8419 5 ай бұрын

Wish i had watched this befire ordering everytbing to build a lam flow hood. Guess ill use both methods. My first attemot at reishis.

@MycoDogs 5 ай бұрын

Flow hoods are definitely a good quality of life upgrade especially if ur doing a lot of work

@babisral 7 ай бұрын

Is it safe to use the induction sterilizer after you spray Isopropyl?

@MycoDogs 7 ай бұрын

I personally haven't had any issues using it directly after spraying (i do it basically every time i use it) so its definitely a lot safer than an open flame but there could be some edge cases that could ignite the fumes so still be careful

@courtneypannell563 2 ай бұрын

You ever melted bag near that induction

@jasonkeehler3470 3 ай бұрын

Wouldnt go back to a SAB after using my flowhood for the past year! Way less tams and way more convenient!

@vornamenachname1015 8 ай бұрын

niiice!

@jamesparker7499 Ай бұрын

Can you put your monotub in a still air box and fruit in it

@gwaters8067 8 ай бұрын

12:16 the gloves looking all unloved 😂

@jobbingactor 21 күн бұрын

If reuse-able glass petri dishes are sterilised in a pressure canner, how to get them into a bag for later use without contamination?

@MycoDogs 21 күн бұрын

I wrap them in tin foil and keep it wrapped til I use them

@jobbingactor 20 күн бұрын

@@MycoDogs Brilliant, thankyou.

@lukeblizzard5909 7 ай бұрын

How do you draw your LC without air exchange? I can only ever get one or 2 before it starts trying to equalize pressure and it sucks air in through the injection port, causing contamination

@MycoDogs 7 ай бұрын

i use a thick layer of RTV silicone as my gasket it seals pretty well but they do wear out eventually and allow air in

@lukeblizzard5909 7 ай бұрын

@@MycoDogs how do you deal with the pressure difference? It gets sooooo hard to draw liquid from the jars I have with good seals if they aren't vented

@MycoDogs 7 ай бұрын

@lukeblizzard5909 it gets kinda difficult but i just hold the slide back when i extract it it gets to a point of waters vapor pressure where it wont get harder to pull after that

@100PercentTHCfilmsX420X 11 ай бұрын

I recently saw online that you can make a liquid culture using a spore syringe as the starter.

@Eliqueme 11 ай бұрын

Def possible,But not recommended

@regenerative1173 6 ай бұрын

Thumbnail says "DUBUNKED" instead of "DEBUNKED". Just mentioning it if you feel like fixing it haha. Grest video, thanks a lot 👍

@yyunko7764 7 ай бұрын

Having the flow hood just removes most of those prep step, it's a huge comfort increase. Of course it comes at a cost, I'd say mine cost around 300€ to build, most of that coming from the fan and filter

@MycoDogs 7 ай бұрын

I'm not saying anything bad about flow hoods, they make workflow faster and easier especially at scale, just most people don't need to spend that much money especially when just getting into the hobby and if they are experiencing contams in an SAB its not because of equipment its more due to their technique and preparations

@yyunko7764 6 ай бұрын

@@MycoDogs That's fair

@jyrueta 9 ай бұрын

Curious - I'm curious, you said at around 1:53 min that you don't encourage others to grow mushrooms for profit. Why did you say that? That was an odd comment to throw in there...

@user-xg3rf9rl6x 11 ай бұрын

I would love to get into mycolagy

@gregscottcampbell 9 ай бұрын

What about spawning to bulk inside a SAB?

@MycoDogs 9 ай бұрын

spawning to bulk doesnt have to be sterile but if u are using a small tub like a shoebox u can do it inside a sab but i just do it on my kitchen floor usually

@Jayhollowood 6 ай бұрын

What I wanna know is the song from 13:50 😂

@MycoDogs 6 ай бұрын

on.soundcloud.com/KC145

@raytry69 4 ай бұрын

6:29 And the air sterilized suddenly. :D

@philipholman9898 3 ай бұрын

Built my laminar blow box for 65 bucks.

@olswole3658 4 ай бұрын

A 6 inch hole saw ran in reverse should cut a quick hole

@legalizefreedom451 9 ай бұрын

Newb here. Why do people sterilize everything with alcohol but always use heat for the blade? Can you just use alcohol? I'm sure there's a reason. I'm just curious.

@MycoDogs 9 ай бұрын

alcohol doesn't sterilize, it sanitizes. to be considered sterile it needs to be devoid of ALL life, isopropyl takes care of like 99.9% of it but that 0.01% means a ton when ur talking about sterility. Nothing survives heat so if u heat the blade you can be sure theres no bacteria endospores or anything hiding on it