DPPH Radical Scavenging Method-Total Antioxidant Capacity Assessment

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DPPH Radical Scavenging Method-Total Antioxidant Capacity Assessment

Рет қаралды 186,983

Күн бұрын

Principle: DPPH(1,1-Diphenyl-2-picrylhydrazyl) is a stable free radial with red color(absorbed at 517nm). If free radials have been scavenged, DPPH will generated it's color to yellow. This assay uses this character to show free radical scavenging activity.

Пікірлер: 128

@IamLilibel Жыл бұрын

Your hand pouring technique is incredible bravo.

@user-di4ge5vh1m Жыл бұрын

Thank you so much!!! This was very useful for my IBDP extended essay research !!!

@saimedhansh8835 11 ай бұрын

Sameeeeeeeeeeeee

@aasifapeer9569 5 жыл бұрын

Wonderful job, systematically created video.

@denaturalseeker 4 жыл бұрын

So great, but it will better if u also explain all detail. How to make standard curve, how many solution and solution to dissolve to make xx concentration in standard curve. I really need the detail

@essaidlekadir9591 4 ай бұрын

The Good results affirmed your great work .

@yashiniselvanathan7828 5 жыл бұрын

very good and detailed described video.. if you have other assays, please share.. will be so useful. Thank you for a such a nice video

@hoangmaianh4650 2 жыл бұрын

thank you so much for your work, it is really a great help to me.

@chaitras153 5 жыл бұрын

Can you please mention the reference for the protocol.

@riyariang2273 2 жыл бұрын

I hv the same question . Did u get the reference ?

@Shrisha-vv6jd 10 күн бұрын

Same. Did you get any references?

@julimdjaffri6100 3 жыл бұрын

Thank you for the informative video. I hope you don't mind me using this to demo to my students. Thumbs up!

@kamon5256 2 жыл бұрын

Thank you. It's very useful🙏🏻🙏🏻❤️❤️❤️

@nctunderrated4031 Жыл бұрын

4:19- sample+methoh 5:22- each sample 1ml mixed with 3ml dpph 6:09- make up with methoh

@kvlogs3212 11 ай бұрын

What is amount of methanol added to the extracted solution

@gcobisamanzane7271 2 жыл бұрын

Thank You very much for the video, I don't see you using ascorbic acid and you listed it in your material to use

@syedashfaq6530 5 жыл бұрын

Fabulous video... Plz share FRAP and MCA also in next videos

@asmaaaboalmagd792 15 күн бұрын

Thanks alot

@TayebeZeinali 3 ай бұрын

Thanks a lot for sharing the video. It's verw wll prepared.

@sarahchahine1244 5 жыл бұрын

Great Video! Could I ask for the references of this protocole? I am combined also about the use of ascorbic acid?

@elijahjadiel1524 3 жыл бұрын

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@juliokylan3775 3 жыл бұрын

@Elijah Jadiel yup, I've been using flixzone for since december myself :)

@draswinjkharka200 4 жыл бұрын

What about ascorbic acid..when is it used? And what is the control sample that has been used during spectrophotometric assay??

@Kesinsanstir 5 жыл бұрын

wonderful thank you

@chanakadissanayake5043 4 жыл бұрын

Great work 💪

@alexbelay8197 10 ай бұрын

great video keep it up

@azharhemeed5045 Жыл бұрын

Amazing 👌

@saiadam473 4 жыл бұрын

great video.looking for ur more videos also i need a video for well diffusion method for antibacterial actvity.

@gustavodmaia 3 жыл бұрын

Thanks for the methodology. However, the results in the table at 7:53 are not the same as in the final graph. If we use the data available, the R^2 of the adjustment is only 0.843. The inhibition values are also incorrects in relation to the absorbance values. Why?

@simplywendy7216 3 жыл бұрын

Because he didn't describe his standard preparation. Calculations are based on results from standard curve which we don't know because he didn't show it

@iftekhar_k Жыл бұрын

%Inhibition curve in not a linear curve as far I know

@arunprasad1442 3 жыл бұрын

What is the use of ascorbic acid? It is mentioned in the materials list but didn't appear anywhere else in the video.

@Nk-nu5sb 3 жыл бұрын

Please mention the reference

@joaohenriquecedraz6308 2 жыл бұрын

Could you mention the refference to the protocol, please?

@alaechda9056 Жыл бұрын

Dpph a simple assay/antioxidant a big term

@sharadham8651 5 жыл бұрын

Can you brief me how to carry out DPPH studies for topical cream

@ghanoking2971 5 жыл бұрын

the referece of this principle please.

@biobienetre1586 2 жыл бұрын

Thanks

@hafizrehan3642 4 жыл бұрын

Good job

@elizabethabiaad6687 3 жыл бұрын

4 mg of DDPH in 100 ml of methanol does not refer to 1M. It is too far from being so.

@anushkadatta6081 5 жыл бұрын

In table there is a column concentration mg/ml. Whose concentration doest it refers?

@oneday8287 2 жыл бұрын

I have the same question, please give me answere??

@angelineb586 4 жыл бұрын

when you were making the solution series..you took 1ml, 2ml, 3ml and 4ml of the sample extract..but how much methanol did you add to it?

@MahmoodHashem 4 жыл бұрын

he completed it to 10 ml (the sign )

@UnknownHakim 2 жыл бұрын

In earlier video, you write ascorbic acid in materials, but i cant see where or when you use in tutorial.

@something.diff. 15 күн бұрын

Ascorbic acid used as control

@something.diff. 15 күн бұрын

Ascorbic acid used as control

@QuynhNhu-hh3ej Жыл бұрын

can someone explain for me where concentration in the result table from? thank youu

@rodolfoalbertolopezpimient7814 2 жыл бұрын

prepararon una solución a 0,1mM no a 1M..... con eso si toman 4 mg

@darker__soul 5 ай бұрын

You don't need a standard ? Usually for antioxidant we take ascorbic acid standard... I'm new to this and kind of confused. Help me

@rimblbz 3 жыл бұрын

Thank you so much, can someone give me the reference please?

@melissaconde7683 6 ай бұрын

Hello, good video, a question, where did you get the reference for the laboratory guide? HELP

@md.arifulhaq7668 2 жыл бұрын

Can anybody explain why his calculated IC50 value is 25 mg/ml while in the table it shows 65% Inhibition at 20 mg/ml?

@elifbedir4664 3 жыл бұрын

What is powdered food sample? What is it in?

@ahmedkhater5369 3 жыл бұрын

what is purpose of ascorbic acid please? because you mentioned to ascorbic acid in materials .

@mumtazhussain3164 2 жыл бұрын

great

@denizdeniz7429 Жыл бұрын

You mentioned acid ascorbic but there isn't in video please can you tell me how you used it? and please here your control was it only dpph +methanol ?? I need the protocol

@anisnabihahzainol1256 4 жыл бұрын

Hi. Im a bit confused where does extracted solution come from, the one that you used to make dilutions? Thank you

@deenmediarecords4575 4 жыл бұрын

ı think it is the filtered solution from the test tubes.

@ranbirkumar5813 Жыл бұрын

Filtered solution

@tnehshuxin9530 10 ай бұрын

@@deenmediarecords4575 the filtered solution is come from one food sample only right?

@pamelabonifacio3643 5 жыл бұрын

Great video! May I ask if you combined the extracts of the 2 samples?

@divyarai7439 4 жыл бұрын

Can you plz give us the reference of your protocol....it would be of great help

@mex3s 4 жыл бұрын

1:43 1mM DPPH ?

@salmamskine8220 4 ай бұрын

0.1mM

@purabiborah9590 2 жыл бұрын

Concentration ka column samja dijiye plz

@sudharsan1735 2 жыл бұрын

Good

@nicoleandreabagas9456 3 жыл бұрын

Reference please?

@sirismily4280 9 ай бұрын

Will you share the research article??

@shilviyabhat6286 3 жыл бұрын

Any refrence

@kokoboop2459 2 жыл бұрын

How could we determine how many mL of solvents to put into the sample?

@rafidahkamaruddin8084 4 жыл бұрын

haii can I know if we use a sample from a liquid ( fruit juice) that same method or different method ??

@vyankateshzambare1440 3 жыл бұрын

Which lab is this (location/name)? I really liked your antique centrifuge and test tube stand.

@wings4749 2 жыл бұрын

Wayamba university of Sri Lanka 🇱🇰

@zainabalial-qallaf6173 4 жыл бұрын

Reference??

@simplywendy7216 3 жыл бұрын

What did you use as your standard?

@puteriindera902 2 жыл бұрын

how do you calculate the concentration?

@arshidsyed6543 2 жыл бұрын

Are you preparing 1M or 0.1mM DPPH...I think it is 0.1mM DPPH you are preparing...

@cheongtzekang4855 2 жыл бұрын

can I know why it needs ascorbic acid for this experiment?

@zygmuntfekner1172 2 жыл бұрын

7:54 Calculate Inhibition% by hand i.e. ((0.301-0.284)/0.301)*100%=5.65% not 5.86%. All values of Inhibition% are incorrect.

@ajaypatange9577 Жыл бұрын

I want cite this vedio, can u give link of this article where it from taken

@nadiatulhusna5943 2 жыл бұрын

Hello, how did you get 1ml, 2ml, 3ml, 4ml and 5 ml for the solution series?

@S3RG1000ful 4 жыл бұрын

excuse me I have a question, the sample showing at the beginning, can be in liquid form???? I would be very gratful if you would answer me

@bhaveshdonda2178 3 жыл бұрын

yes it can be in liquid form so that eliminates the whole process of extraction and then you just have to work from the making the series dilution process.

@lakykhatun7119 2 жыл бұрын

Where is the standard solution?

@raabikhan8447 3 жыл бұрын

where is ascorbic acid function n method ?

@Pihu_dosanjh 2 жыл бұрын

May i know which food sample you used in your study

@anayaanaya6206 5 жыл бұрын

?plz the color yellow is obligatory te confirm the effect or not

@biologicalmasti6718 2 жыл бұрын

Sir Please provide me flowchart of dpph assay, please🙏🙏🙏🙏

@akshatakadam5665 7 ай бұрын

Any other solvent than 99% methanol that can use???

@erasse100 3 жыл бұрын

Hi. When is it used ascorbic acid?

@imedsam5021 4 ай бұрын

what did you make in sample, and negative control

@keerthikeerthi8327 2 жыл бұрын

Which book you can get procedure ..name pls ...

@alipchia802 5 жыл бұрын

What is control? Is it sample abs without dpph or dpph only?

@aproblem6ix889 5 жыл бұрын

Alip Tanpanama it’s the one with ddph and ethanol without your sample

@rabiyamehandi6354 5 жыл бұрын

control is DPPH abs.

@Anonymous_Kannadathi 4 жыл бұрын

Please tell us the scientist name whose methadology you used for doing this experiment... please tell us,its so necessary to do this experiment.please text us the reference.please

@dienkimotaung7167 4 жыл бұрын

Hi did you get the references?

@eswaryam2660 Жыл бұрын

What is the blank in the spectrometer?

@HARHGNK 4 жыл бұрын

what is the blank composition

5 жыл бұрын

where's the paper?

@k.arshdeep2601 4 жыл бұрын

Can you please share the %Inhibition for iceberg lettuce using the same dpph assay?

@grantgabrieltollo9866 2 жыл бұрын

😂😂😂

@amnazahid990 2 жыл бұрын

I wanted to ask something.....is 58% a good antioxidant value?

@MestebanK 2 жыл бұрын

the question is what was the concentration of the sample you tested... later on you can check what is the parallel concentration of a known molecule (like vitamin e for example)

@amnazahid990 2 жыл бұрын

@@MestebanK my sample was 3ml

@nasytadifafirdaus3477 2 жыл бұрын

DPPH 4 mg is real at that weight??

@pradeepkumarpanda5559 5 жыл бұрын

are samples soluble in DPPH solution?

@otabila3830 2 жыл бұрын

Reference?? Please.

@anubhibadhani1731 2 жыл бұрын

what you take blank solution?

@abdulganiyumuktaribrahim9697 4 жыл бұрын

Any other methods?

@mehtajaydip6884 4 жыл бұрын

Sir please send dpph data sheet

@Sandhyaranibhagawath 9 ай бұрын

Hlo please provide any reference link

@mr.crossover6633 4 жыл бұрын

What's the purpose of using methanol?

@HARHGNK 3 жыл бұрын

organic solvent

@Rizuk0 4 жыл бұрын

Hi, can anyone help? just want to ask for this DPPH method, is it a must for using a methanol? I mean , we cant do it directly using only the dpph then adding the sample? #stilllearning

@maiaothanh9480 4 жыл бұрын

No, it is must for using methanol or ethanol.

@Rizuk0 4 жыл бұрын

@@maiaothanh9480 currently I'm doing my thesis, and yes, it's a must using either methanol or ethanol

@chemistry_live 3 жыл бұрын

no, not necessary

@STALKER1807 3 жыл бұрын

@@Rizuk0 Hello, im currently on my thesis as well, i might ask you what's the difference between using methanol or ethanol (I know methanol gives better performance during the extraction, but whats the use in here in comparison with ethanol)

@Rizuk0 3 жыл бұрын

@@STALKER1807 Hello ahmed, as for the comparison of both, I'll have to suit it up accordingly to the thesis, when it comes to DPPH assessment for some studies both solvent affects the antioxidants, and these would be one of those consideration ; using one of solvent could affects the DPPH environments for reduction, may some solvent makes the DPPH unstable as it does when reacting with the antioxidants vice versa ; solubility of both solvent should be different, thus affecting the absorbance. Hopefully these answer could useful for you.