Episode #10 - What Is A Fluorescence Minus One Control?
Рет қаралды 3,650
7 жыл бұрынThe Fluorescence Minus One Control, or FMO control is a type of control used to properly interpret flow cytometry data. It is used to identify and gate cells in the context of data spread due to the multiple fluorochromes in a given panel… (Read more below.)
Click here to get our FREE ebook, Modern Flow Cytometry: www.expertcytometry.com/ebook
An FMO control contains all the fluorochromes in a panel, except for the one that is being measured.
The FMO control ensures that the any spread of the fluorochromes into the channel of interest is properly identified.
Click here to get our FREE ebook, Modern Flow Cytometry: www.expertcytometry.com/ebook
@kirstenredman9394 5 жыл бұрын
Thank you so much for this, Tim! I'm busy optimising a panel at the moment, just finished titrations and my next step is FMO's! I last did them in my honours year, now its PhD, all by myself and its a little bit daunting. This helps. ~South Africa
@borjeism 5 жыл бұрын
I'm having trouble understanding the concept of this, and how does this differ from compensation control. Compensation matrix when applied should also 'remove' the spillover from other fluorochromes into the channel of interest? Why should the spillover be different if there are multiple fluorochromes all at once, or if we calculate the percentage of each fluorochrome spillage with single stain control? What would happen if I applied compensation matrix to FMO stained control, just out of curiosity?
@expertcytometry6235 5 жыл бұрын
Borjeism, Compensation controls are tubes with a carrier stained with a single fluorochrome. This is used to calculate the spectral overlap and generate the compensation matrix. The FMO control is a sample (cells) stained with all the fluorochromes but one so that overlap into that channel can be visualized and the gates properly applied. This is to identify the spread of the data.
Braincatchers
Рет қаралды 12 МЛН
BioLegend
Рет қаралды 4,6 М.
Spectral Instruments Imaging
Рет қаралды 1,1 М.
Biology Lectures
Рет қаралды 131 М.
Syncswift
Рет қаралды 1,8 МЛН