Exploring the fascinating development of cannabinoid-producing trichomes - Zamir Punja, PhD

  Рет қаралды 267,198

Medicinal Genomics

Medicinal Genomics

2 жыл бұрын

Tags: cultivation, potency, cannabinoids, terpene, resin, cannabis growing, yield, harvest
Zamir Punja presents “Exploring the fascinating development of cannabinoid-producing trichomes in cannabis flowers” at CannMed 2022.
Glandular trichomes produced on bracts of female inflorescences of THC-containing plants are known to produce cannabinoids. What are the details of how these structures develop ? The fascinating process of capitate stalked trichome development was studied using light and scanning electron microscopy (SEM). Two strains- Moby Dick and Space Queen - were compared at 3 weeks and 6 weeks of the flowering period. Four morphologically distinct trichome types will be shown: nonglandular hairs (stacoliths) and three glandular (secretory) forms -pedicillate bulbous (15-30 um in diameter), sessile peltate (30-45 um in diameter) and capitate (40-85 um in diameter) that were stalked or sessile. The stalked capitate trichomes are formed in greater numbers on lower compared to upper bract surfaces, and at higher frequency on Space Queen compared to Moby Dick as maturity of the inflorescence progressed. Trichome glands increased in volume with maturity and resin was secreted through cracks or tears in the surrounding cuticle, resulting in collapse of the trichome gland. Dried buds of four cannabis strains showed varying trichome densities, stages of development, trichome stalk length, and degree of gland dehiscence. These findings may be extrapolated to develop a method to estimate trichome gland maturity to optimize cannabinoid levels at harvest.

Пікірлер: 460
@billlumberg5746
@billlumberg5746 Жыл бұрын
I have been taking pictures of trichomes with microscopes for like 15 years. But Wow these pictures are phenomenal amazing stuff doc.
@_cspr
@_cspr Жыл бұрын
@@Bombarded1n this is about real weed not no detla 8 shit
@borbleborb4586
@borbleborb4586 Жыл бұрын
@@_cspr delta 8 is hemp, not cannabis 😂
@_cspr
@_cspr Жыл бұрын
@@borbleborb4586 im well aware lol
@borbleborb4586
@borbleborb4586 Жыл бұрын
@@_cspr just sayin that’s a weird thing to pull outta nowhere lmao
@_cspr
@_cspr Жыл бұрын
@@borbleborb4586 superstrain is a delta 8 brand, why i said it to the guy talkin about superstrains for whatever reason
@morgan1719
@morgan1719 9 ай бұрын
Who is going to be the hero who captures a microscopic time-lapse of trichomes growing/maturing. He'll get the nobel prize
@korkee1111
@korkee1111 Жыл бұрын
is this what happens when a scientist gets a really really really good bag?
@bennymartinez5532
@bennymartinez5532 Жыл бұрын
😂😂😂😂😂😂
@rareroots
@rareroots Жыл бұрын
Some of those magnified images belong on a wall as posters, beautiful
@zamirpunja1978
@zamirpunja1978 Жыл бұрын
I actually have a 12 month calendar made up. Each month is a different trichome photo ! Pretty awesome as you wait for the next months image to show up !
@pat8988
@pat8988 Жыл бұрын
@@zamirpunja1978 You should sell them!
@moleisrich1
@moleisrich1 Жыл бұрын
Holy trichomes Batman. Wow wow wow
@FarmerRCA
@FarmerRCA Жыл бұрын
Beautifully detailed trichome presentation 💚🙏
@craigshaw1886
@craigshaw1886 Жыл бұрын
Hot Crust Buns! I love the science and research going on, and the fact that there is some much left to discover.
@bennymartinez5532
@bennymartinez5532 Жыл бұрын
I watched this video in the morning drinking my coffee. Had a good laugh when he used hot crust buns to describe two bulbs stuck together 😂😂
@SomeonethatsNoOne
@SomeonethatsNoOne 8 күн бұрын
The visuals really helped me grasp the concept better. Incredible research and breakdown here, thank you for this!
@ziiziizii3838
@ziiziizii3838 Жыл бұрын
I wonder what they did with "The study material" after because it looks amazing.
@ezelrik
@ezelrik Жыл бұрын
Great stuff. I just love it when the former hippie plant becomes scientifically explored. Keep em coming!
@jimmorrison8477
@jimmorrison8477 Жыл бұрын
Former hippie plant wow so ignorant
@williambarringer6513
@williambarringer6513 10 ай бұрын
The first “acid test” grateful dead shows were the first attempt at a “Meta-verse” where they provide you with their version of reality, they’re bohemian grove members
@burgerbobers
@burgerbobers 9 ай бұрын
When a former hippie plant what other hippie plants you talking about?
@sprocastersprocaster
@sprocastersprocaster 9 ай бұрын
@@williambarringer6513 Please elaborate
@ThatOpalGuy
@ThatOpalGuy 7 ай бұрын
but GAWD gave us this plant and the evil goobermint stole it from us
@The-KP
@The-KP Жыл бұрын
The energy going into filling the globular trichomes with enzymes, cannabinoids and THC.. all part of the specie's propagation and survival strategy.
@Egzoset
@Egzoset Жыл бұрын
Once in a while the work seems quite noticeable, IMO that's one rare sample right here.
@Breca
@Breca Жыл бұрын
Yes I was *fascinated* computers will bring us the best cannabis ever. Thanks Mr. Z. Punja
@cadarok1079
@cadarok1079 Жыл бұрын
Can't wait to see more development on this.
@DennisMoore664
@DennisMoore664 Жыл бұрын
This is one part of the future that I am loving!
@insideaero7660
@insideaero7660 Жыл бұрын
Thank you very much for the pictures and information.
@becadinellijotaeff7641
@becadinellijotaeff7641 Жыл бұрын
My main english is not even english, im a biology student, you speak so easily and so precise, and the images are fabulous!! Thank you so much!!
@libertyblueskyes2564
@libertyblueskyes2564 Жыл бұрын
The most intelligent and beneficial plant on the earth!
@molochfrolics8443
@molochfrolics8443 2 ай бұрын
Banisteriopsis caapi says 'hello'
@noway905
@noway905 9 ай бұрын
Makes me want to go light up, but a nap seems more appropriate. I'll push pause, do the nap thing, wake up, light up and finish enjoying this flick.
@jakelooter5139
@jakelooter5139 9 ай бұрын
no one cares
@samsham8218
@samsham8218 Жыл бұрын
WOW!!! Spectacular! Extremely fascinating.
@carolynadams3994
@carolynadams3994 Жыл бұрын
Experience is your best teacher! Go for it!
@ttodtmann
@ttodtmann Жыл бұрын
Thank you very much for the science and for sharing information.
@DG-iw3yw
@DG-iw3yw 5 ай бұрын
Got to love how it has taken modern science to tell us what near eastern farmers have known for centuries
@Laserblade
@Laserblade Жыл бұрын
I can't help but wonder what evolutionary pressure or benefit caused the plant to develop the trichomes?
@insideaero7660
@insideaero7660 Жыл бұрын
Afaik the trichomes are a natural shield/reflector against radiation. The plant comes originally from the Nepal/Hindukush area. Means, she had to fight the radiation of the sun for propably millions of years. In my opinion it is some kind of a natural evolutionary developed shield against UV and other to biological lifeforms hazardous radiations (be it from the ultra short or ultra long spectrum of light). Well, that is at least what i got from reading deep into it back in the days. I was already growing in the 90s, and i remember that we already had studies from scientists floating around at that time. Cant remember specific names, but i definitely know that science researched that already 20-30 years ago. If i remember an article or scientific paper about it, then ofc i will add it here as comment.
@Laserblade
@Laserblade Жыл бұрын
@@insideaero7660 You know, that make perfect sense, there would have to be a shading effect from the quantity. Thanks!
@mkx9095
@mkx9095 9 ай бұрын
God
@jakelooter5139
@jakelooter5139 9 ай бұрын
seed protection OBVIOUSLY they are extremely coveted by animals for food.
@greatgardenguru8718
@greatgardenguru8718 Жыл бұрын
what a wonderful, thourough and educative video!! It wouldve been nice to see how different the trichomes differ im a Sativa/Imdica..but seriously as a seasoned cannabis grower this just blew my mind. I learned so much more about trichomes!!! and cannabis itself.. best video ever! subbed!!
@Armistead_MacSkye
@Armistead_MacSkye 9 ай бұрын
"Educative" 🤔
@greatgardenguru8718
@greatgardenguru8718 9 ай бұрын
@@Armistead_MacSkye 🤡
@mrjuli8314
@mrjuli8314 Жыл бұрын
Amazing. Thank you so much for the information and the images
@calimark7448
@calimark7448 Жыл бұрын
Wow! Thanks! Amazing info. More please!
@KnutKniffte
@KnutKniffte Жыл бұрын
Wow, thank you for summing it up so nice.
@scottturner2529
@scottturner2529 Жыл бұрын
Nice work. You’ve answered several questions that have been swirling around my head. Great images too.
@kimslater2910
@kimslater2910 Жыл бұрын
Great video. Very educational..love tge the electron microscope images..thx fir sharing
@aegrotattoo9018
@aegrotattoo9018 8 ай бұрын
Absolutely gorgeous. Shows all the beautiful structures removed and destroyed in legal weed. Would be nice if they ever matched the black market in price and quality, but not holding my breath, no pun intended. Thank you for such amazing views of this fascinating plant.
@whiteowl8703
@whiteowl8703 Жыл бұрын
Very well done. Thank you. Look forward to more info from you. 😊
@adultpersonman4612
@adultpersonman4612 Жыл бұрын
Amazing video! I really appreciate you breaking down the structures so thoroughly. You mentioned the enzymes in the trichomes that manufacture terpenes and cannabinoids, and I was wondering you knew any papers or research that went into detail about that? How plants manufacture chemicals like these has been fascinating to me for awhile, and I hope to do research on it in the near future. Thank you again!
@jakelooter5139
@jakelooter5139 9 ай бұрын
Have you heard of google? or incapable of reading and doing basic research...
@adultpersonman4612
@adultpersonman4612 9 ай бұрын
@@jakelooter5139 why the need to be so condescending? I have looked it up before, and since leaving this comment I have researched it quite a lot. I was just asking for help from professionals, whose whole channel is dedicated to sharing information on cannabis.
@yourfreedom2037
@yourfreedom2037 Жыл бұрын
Tremendous and interesting exploration, as a grower I have always been impressed by the cannabis plant and how intelligent its whole structure is, of course all plants have amazing systems, but cannabis has that special susbstance, I have taken very good pictures of the trichomes when the plants are in the sun and these trichomes in the telescope look great, from now on I will follow the channel because I got very good information from this video.
@websurfer5772
@websurfer5772 Жыл бұрын
You mean scanning electron microscope 😀
@SouthpawPablo
@SouthpawPablo 10 ай бұрын
@@websurfer5772 (slow clap) thank you!!! this isnt the same as using a jewelers loupe to check if harvest ready or using a 1000x to check for other things or just see progress this is an electron microscope something the vast majority of us will never have access to so value that aspect also its an amazing machine
@phil331
@phil331 Жыл бұрын
fascinating lecture. love this incredible plant
@newyorkfilharmonik110
@newyorkfilharmonik110 Жыл бұрын
Excellent!
@forestknowledge
@forestknowledge Жыл бұрын
Can you do a video like this for Psilocybe Cubensis? Another completely beautiful organism with such powerful molecules ❤️
@SjMk1.
@SjMk1. 9 ай бұрын
yes to shrooms 💯
@ThatOpalGuy
@ThatOpalGuy 7 ай бұрын
nature has the BEST molecules, man
@melrezlittleranch
@melrezlittleranch Жыл бұрын
Thank you for making this video. Such beautiful photos.
@UAPG007
@UAPG007 Жыл бұрын
Thank you this was Great!
@instargramfirmament1887
@instargramfirmament1887 Жыл бұрын
Fantastic thanks a lot !!!
@alexandrerichard6057
@alexandrerichard6057 Жыл бұрын
Superb imagery
@ThisIS_Insane
@ThisIS_Insane 9 ай бұрын
Thank you, Doctor! I love this stuff!
@jackbuff_I
@jackbuff_I Жыл бұрын
There is a story from the Dogon tribe that says cannabis was a gift to the earth from beings that are from the Canis star system. And looking at this beautiful SEM pictures of the Trichomes it's easy to believe! .. it's worth remembering that that Tribe were able to describe star systems and constellations a looooong time before humans knew about them. Makes you wonder where they got that info. 🤔
@kika-ge5qr
@kika-ge5qr 11 ай бұрын
Thoth the Atlantean, maybe?
@tomi-jon8798
@tomi-jon8798 5 ай бұрын
Im here from the Madame Cannoli live stream !! Oh man can you imagine these growing in ours streets!! I would be a very happy dude.
@sum1namedjon
@sum1namedjon Жыл бұрын
Awesome talk! Great images!
@alexindustrials8742
@alexindustrials8742 9 ай бұрын
Masterpiece, I loved your research One of its kind
@terrormilk384
@terrormilk384 Жыл бұрын
you have such a clear way of communicating this information to us, this is why i love scientists their minds work with such conscientiousness
@jackbuff_I
@jackbuff_I Жыл бұрын
Why don't they shake the resin off? I know it's sometimes laborious but it's gorgeous! This plant is so soooo helpful for humanity! It's seriously the most beautiful thing in the world!
@mkx9095
@mkx9095 9 ай бұрын
Actually they do like that once is dried .
@jonhutchinson2902
@jonhutchinson2902 Жыл бұрын
Very intersting. Great images and information
@ChiTownTino
@ChiTownTino Жыл бұрын
Re: trichome density on bottom. Maybe because insects tend to prefer the undersides of leaves. Just a guess. 22:02
@R3ALCHANN3L
@R3ALCHANN3L Жыл бұрын
very informative, super awesome! thank you for this
@tommybank
@tommybank 8 ай бұрын
Awesome video! Some much great info.
@vomeronasal
@vomeronasal Жыл бұрын
Excellent talk! I am a molecular virologist and geneticist turning his eyes towards cannabis research...
@websurfer5772
@websurfer5772 Жыл бұрын
Can you please tell me, has any scientist ever actually seen a virus? I'm dying to know. Also, has anyone ever seen a double-helix, twisted, DNA molecule?
@silver_salvage_savage
@silver_salvage_savage Жыл бұрын
I would think there are more glands on the bottom because gravity works for free. The really long trichome stalks are prob just stretching up out of the forest for light? Fascinating slides. I didn't know there were tricomes that never develope heads. Thought I was being too rough in handling.
@randallturner3056
@randallturner3056 Жыл бұрын
absolutely wonderful work
@kosmique
@kosmique 9 ай бұрын
what an amazing video. thanks for the presentation!
@st.rv888
@st.rv888 Жыл бұрын
Outstanding presentation, excellent and interesting.
@Non-dual-mind1
@Non-dual-mind1 Жыл бұрын
Tetrahydrocannabinol is only one of the 113 total cannabinoids on the plant. Trichome glands are our friends.
@darkstar4214
@darkstar4214 Жыл бұрын
Excellent video!
@fullmeltxtractions2645
@fullmeltxtractions2645 Жыл бұрын
I was already pretty familiar with the structure with glandular trichome head and the capitate stalk. These images are absolutely amazing though. It really gives me an amazing real life window into what these structures look like up super close. Also shows me the reason why that no matter how well you isolate the heads, the resulting hash will never quite melt down to no solid residue. Even with perfectly isolated heads, you still have those stipe cells and secretory cells on the underside that are not going to be melty. Even if you find a crazy mutation where those cells are super small, I'm guessing they'll still take up at least 10% of structure of the head. Which is why you can never get 100% melty product unless you blast it with butane or some other hydrocarbon, or press rosin out of it, to sort out the non-melty bits.
@Hurricanehamo
@Hurricanehamo Жыл бұрын
Tres bien. Thank you.
@Dr3winth3Grim3s
@Dr3winth3Grim3s Жыл бұрын
Thank you for the presentation and sharing your knowledge
@johnboykin3128
@johnboykin3128 Жыл бұрын
Thank you ❤️
@mrmaestrouk
@mrmaestrouk 9 ай бұрын
This is Brilliant. Spliff time
@armandocervantes6142
@armandocervantes6142 10 ай бұрын
pretty well done.... full of knowledge to get the right education.......thank you very much ......
@prowlermadmax2
@prowlermadmax2 9 ай бұрын
Great job, love you're video.
@RandyRhoadsFL
@RandyRhoadsFL Жыл бұрын
Awesome video thank you
@shucksful
@shucksful 10 ай бұрын
Outstanding work, sir.
@loonyloony6550
@loonyloony6550 Жыл бұрын
Seeing those pics makes me giggle with joy!
@deanlauer6950
@deanlauer6950 Жыл бұрын
I Would I like to see as a agronomist and horticulturist that some of hybrids be used in the landscape industry. Of course without the THC . But still provide the colors and fragrance. Unlike flowers and other flowering plants, cannabis holds its fragrance for almost the life of the plant . Something to think about
@nickhowatson4745
@nickhowatson4745 Жыл бұрын
it quickly and aggressively chokes out all other species though which makes gardening somewhat difficult.
@donilexington4600
@donilexington4600 Жыл бұрын
Thc is the best part!
@mkx9095
@mkx9095 9 ай бұрын
Cbd flowers dude
@jakelooter5139
@jakelooter5139 9 ай бұрын
no it doesnt. it doesnt even smell until near the end, when the terpene profile changes daily and weekly. Why no THC? As a "horticulturist" LOL you should know, that it is the SAME SPECIES Cannabis sativa, ie NO HYBRIDS genetically. all of you fake scientists on here trying to look intelligent because you are pseudointellectuals w barely above average IQs...and know it. No one cares what you want to see either FYI...
@jakelooter5139
@jakelooter5139 9 ай бұрын
@@nickhowatson4745 no it doesnt most weeds will overtake it in a fertile environment...
@user-xy6ei6wq2u
@user-xy6ei6wq2u 9 ай бұрын
Very cool! Answered a lot of questions I’ve had!
@notbeefyproductions7109
@notbeefyproductions7109 Жыл бұрын
Thankyou Zamir Punja for this Excellent Presentation,Loved it :)
@panariellomarco
@panariellomarco 2 жыл бұрын
Very good presentation. 🙏
@TheMooseintree14
@TheMooseintree14 Жыл бұрын
😊 ❤ God is amazing, look at his creation.
@CNCTEMATIC
@CNCTEMATIC 10 ай бұрын
Makes sense to me that there'd be more trichomes under than on top. Cannabanoids are partly pest replenents, as I understood it. And most pests will move from lower on the plant to higher.
@wanderingknight10
@wanderingknight10 Жыл бұрын
Exceptional
@carlallen4904
@carlallen4904 Жыл бұрын
Would have been interesting to compare outdoor, vs indoor trichomes!
@papacos4745
@papacos4745 Жыл бұрын
I agree. I bet with the outdoor you would see many different dead insects that got stuck inside these trichomes
@catalunyaluke6881
@catalunyaluke6881 Жыл бұрын
it could be awesome
@zamirpunja1978
@zamirpunja1978 Жыл бұрын
Hi Carl - yes I have not looked at outdoor flowers to see the trichomes. They could look quite different, being exposed to different environmental stresses.
@coolboy5428
@coolboy5428 Жыл бұрын
@@papacos4745 I get fruit flies on my buds which are indoor, outdoor UK not many pests but snails nibble a bt but thats ok, snails gotta eat too!
@Breca
@Breca Жыл бұрын
@@papacos4745 Yes like looking into amber
@kevinwilson2082
@kevinwilson2082 9 ай бұрын
(Artists conception of a glowing 4 meter tall trichome) “It would be wonderful to see these on our streets...”. No shit. I love this guy!!
@specificgravity-thedancing9700
@specificgravity-thedancing9700 9 ай бұрын
Holy wow is this the best thing I've seen in years. I love marijuana and I have a degree in plant science from Penn State! I love this stuff, thanks so much!!
@Wicked_maniac
@Wicked_maniac Жыл бұрын
This was a very fascinating video
@richarddehn4552
@richarddehn4552 9 ай бұрын
What are trichomes produced for? what is their function for the plant? What environmental factors influence growth/production? Are they to deter or attract ?
@Gilky781
@Gilky781 5 ай бұрын
excellent presentation
@elizabethwilliams6109
@elizabethwilliams6109 9 ай бұрын
Thanks!
@jefftaylor4062
@jefftaylor4062 Жыл бұрын
Awesome 👍🏽
@eddiesland93
@eddiesland93 4 ай бұрын
Very interesting science behind cannabis
@strictdrugregiment
@strictdrugregiment Жыл бұрын
Cannabis trichomes are so cool.
@gbeagle417
@gbeagle417 Жыл бұрын
Thank you
@jedz86
@jedz86 Жыл бұрын
thaank you sir
@mr.wookiesack
@mr.wookiesack Жыл бұрын
I stopped taking clones of a Cali gold strain that grew amazing, looked good, but it didn't have any smell. I grew 4 or 5 clones. Then bloomed the mother. I sifted most of the bud for kief and it made the craziest kief I e ever seen. It all melted. My theory was the gland heads perfectly fell off. I regretted blooming the mother.
@jakelooter5139
@jakelooter5139 9 ай бұрын
they always fall off,,,
@mr.wookiesack
@mr.wookiesack 9 ай бұрын
@@jakelooter5139 well then you must be a great hash maker. if they all fell off everyone would have super pure rosin and hash
@capiloko9026
@capiloko9026 2 ай бұрын
nice video, thanks
@neonninja5049
@neonninja5049 Жыл бұрын
Very cool to see at that magnification.
@Egzoset
@Egzoset 2 ай бұрын
Imagine with colours. Microscopes can be cheap and i never had regrets buying mine, except for the mechanical support with no easy focus...
@neonninja5049
@neonninja5049 2 ай бұрын
@@Egzoset everything at that magnification level is just amazing the world of the very small is just out this world it’s alive I’m interested in nano technology and life at this scale. We need to focus on this because it can really help us but it’s the tools 🛠️ to play with our nano world we also have to be very careful not to disturb it as it can cause massive problems like with gain of function research.🔬
@orbiebibbee2998
@orbiebibbee2998 Жыл бұрын
Look so similar to mushroom formations. Tiny explosions of potential.
@jakelooter5139
@jakelooter5139 9 ай бұрын
Not really...I am a widely known mycologist NOT a random idiot posting
@andrewnorgrove6487
@andrewnorgrove6487 Жыл бұрын
I've always grown outside the last near 50 years and always used Female rather than going through the process of male & female My biggest problum being thick Buds ! Due the state I'm in around march humidity shoots up leading to mold ! I use the Trichome to decide when the buds are ready from a clear or look to the foggy look they get towards the end ! That way you can pick maximum THC production along with all the other traces / cannabinoids My next import requirement for me is the Taste and smell / terpenes ,The electron magnifying Puts my $100 one to shame But the same process ! Always the happy receiver of gifts if you feel the need to send your electron to Australia , great video thank you
@jakelooter5139
@jakelooter5139 9 ай бұрын
microscope begging on youtube? literally one of the dumbest comments I have EVER seen on the internet period.
@johnbillings5260
@johnbillings5260 9 ай бұрын
Beautiful images! Most non-users don't get it I'm sure.
@timsmyth7942
@timsmyth7942 9 ай бұрын
harvested at 7 weeks, dried for 5 days then sold. thats why you grow your own 💀
@boblee4000
@boblee4000 Жыл бұрын
At 7 weeks these plants have the potential to develop further
@LINLEY1
@LINLEY1 9 ай бұрын
Beautyfull.....enlightening......new.....deserving of a big screen view,.......also...how about a video of it growing?
@ace2044
@ace2044 11 ай бұрын
Wow incredible pictures. I was imagining myself as a miniature man walking around this alien trichome atmosphere
@jackbuff_I
@jackbuff_I Жыл бұрын
The difference in trichomes on the underside must be to reduce any compromise and/or negation of photosynthesis, kind of in the same way we put black plastic balls in bodies of water to negate evaporation from sunlight.
@wallyblackler46
@wallyblackler46 Жыл бұрын
I like my tri-combs to be looking like a snowy hill before I pick them
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