This is the first of a 2-part series on compensation which supplement the Flow Basics class offered at the CAT Facility.
Compensation has been discussed in details over the years, and you'll find a number of material online to help you better understand this function:
Mario Roederer's compensation page: www.drmr.com/compensation/
Compensation in Flow Cytometry (2002): doi.org/10.1002/0471142956.cy...
To learn more about unmixing and Spectral Flow : • Cytek Aurora Training ...
The basics of the spillover issue- 0:00
Manual and automated compensation - 9:37
Compensation Control Rules - 16:38
Reusing your compensation matrix / tools to predict spillover - 21:55
In the next episode - 26:14