This video is the third part of the lecture on flow cytometry and hematopoiesis. In today’s video of the flow cytometry series, we will be covering step by step journey of B-cell hematopoiesis and maturation with changes in CD markers and also the origin of all major B-cell neoplasms including B Lymphoma and acute lymphoblastic leukemia.
• Flow Cytometry - 4 | C...
the first video on flow cytometry:- • Flowcytometry Basics -...
the second video on flow cytometry:- • Flow Cytometry - 2 | H...
the third video on flow cytometry:- • Flow cytometry -3 | A...
the fourth video on flow cytometry:- • Flow Cytometry - 4 | C...
If you have missed our previous videos on the basics of flow cytometry where in part 1 we discussed working principles of the flow cytometry machine, interpretation of graphs, In part 2 of the flow cytometry series, we understood what CD markers are, and how hematopoiesis and cd markers lay down the foundation for understanding flow cytometry and interpretation of flow cytometry plots. We also learned cd markers of myeloid series on flow cytometry plots. If you have missed our previous two videos, there is a link you can see up, do watch them for having a better understanding of this flow cytometry lecture on hematopoiesis and B cell development and in no time, you can become a champion in the interpretation of flow cytometry
B-cell neoplasms are clonal tumors of mature and immature B cells at various stages of differentiation. Each b lymphoma and acute lymphoblastic leukemia subtype bears a phenotypic resemblance to B cell at a stage of differentiation during the hematopoiesis.
So instead of mugging different cd markers for b lymphoma and acute lymphoblastic leukemia, if we just remember the normal postulated counterpart from which b lymphoma and acute lymphoblastic leukemia arises, flow cytometry plots will NEVER BE A BRAIN TEASER AGAIN.
Now let’s move to the part we all want to learn- that is diagnosing b lymphoma on flow cytometry. Diagnosing b lymphoma and acute lymphoblastic leukemia by flow cytometry is a challenge. Let’s understand why?
Many a times in flow cytometry we get a sample from a lymph node or a peripheral blood to be tested for presence of lymphoma . However lymph node might be having reactive lymphoid cell proliferation due to infection , or peripheral blood lymphocytosis may again be reactive . So how do we differentiate both the conditions on flow cytometry plots? The answer is to learn the must know CD markers!!
Each lymphoma subtype bears a phenotypic resemblance to B cells at a particular stage of differentiation. So instead of mugging different markers for lymphomas , if we just remember the normal postulated counterpart from which lymphoma arises , identifying LYMPHOMAS on flow cytometry WOULD NEVER BE A BRAIN TEASER AGAIN.
So that’s all for today in flow cytometry. We sincerely hope that after listening to this video on flow cytometry in b lymphoma and b acute lymphoblastic leukemia, you all will be able to suspect and diagnose them on flow cytometry plots. If any doubts or queries regarding flow cytometry, please do write to us in comments section and we will love to hear from you. If you have not subscribed , make sure you do now so that you don’t miss your updates in oncopathology and hematology.
For basics and working of flow cytometry machine and interpretation of flow cytometry plots , you can watch our first video on flow cytometry series, a link of which is given in description below.
We hope you liked our video and stay tuned for next video on how to identify t cell lymphoms and t acute lymphoblastic leukemia on flow cytometry.
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