Flow cytometry for DNA analysis

Рет қаралды 69,000

Marcos Garcia-Ojeda

Күн бұрын

Пікірлер: 30

@sanggillee6861 7 жыл бұрын

So far, the best lecture I found about Flow cytometry for cell cycle analysis. Thanks.

@marcosgarcia-ojeda1066 7 жыл бұрын

Glad you found it useful!

@MrMRbarati 2 жыл бұрын

I have the same comment. Short, consize and right the point. Thanks

@drummeratheartt 7 жыл бұрын

This is incredible! Thanks a million :)

@charlesidu-okojokwu9732 2 ай бұрын

Thanks in a million sir.

@sinamt2982 Жыл бұрын

You explained that topic so well. Thank you so much!!

@rebeccaandersson6312 7 жыл бұрын

This is great! I have been searching for this information and I finally found it. Thank you!

@marcosgarcia-ojeda1066 7 жыл бұрын

You are welcome. I'm glad you found it useful.

@aamnailyas8246 4 жыл бұрын

Thank you so much. It helped me alot

@quintabrownderiona702 3 жыл бұрын

Thank you so much!

@dstan16224 Жыл бұрын

Thank you very much! Really helped a lot...

@chairulyahya66 6 жыл бұрын

Nice lecture..so I understand more about it, thank very much

@tatyanazilberman5256 4 жыл бұрын

great ! Thank you

@jlee9852 5 жыл бұрын

clear ，thanks！

@missroua6862 7 жыл бұрын

thank u so much it is very useful, simple and deep (Y)

@divyanshutiwari9916 2 жыл бұрын

The video has explained the content in a very detailed manner! I wanna know, how can the duration of a particular phase is correlated with the number of cells?

@ranabirmajumder1840 6 жыл бұрын

Simple but very effective lecture... Thanks. :)

@jacovisagie4457 8 ай бұрын

I have a question. If all DNA has the same negative charge, how would it then be able to sort for example aneuploidy cells from diploid cells?

@smitshah8074 3 жыл бұрын

thank you sir

@job506 10 ай бұрын

Many thanks. Please, my cell cycle statistics is more than 100%. Why, please?

@pramitlahiri1978 4 жыл бұрын

I can't understand why the cell count drops so drastically. The last example that you presented, at G1 the count was 1100, then at S it was around 100, at G2 it was around 110, so cell count had decreased by 10 times. Why? Do explain. Thanks!

@martinbatalla3650 2 жыл бұрын

It is just an example, depending what type of cell and from which organism you are working on, you could see different peaks. In this example, these cells seem to spend more time in the G1 phase, so that's why we see such a peak at G1; however, there still are some cells at the S and G2 phase, which is the rest of the curve, but clearly not as much as in the G1 (this is because these cells seem to spend less time in these stages). I think that for the most part, cells follow this timeline, but some cells like those of yeast spend much more time in the G2 phase; if we were to work on them we would see a bigger peak in G2.

@drrakeebahmad8736 4 жыл бұрын

In a graph it is mentioned that no. of cells vary from G1>S>G2, represented in Y-axis. Please explain this aspect...

@makeminecaffein8ed 4 жыл бұрын

it is a tally of cells in each phase within a population of asynchronous cells. e.g. most cells in a population will be in G0/G1 part of the cycle.

@drrakeebahmad8736 4 жыл бұрын

@@makeminecaffein8ed thanks

@victorleon3925 3 жыл бұрын

I keep observing that they begin with N or with 1C. Are you suggesting that gametes duplicate their genome? how is that possible? YOu mean from 2N to 4N

@aysibrahim4739 6 жыл бұрын

I have a q , why do all cells atthe begaining have the same amount of DNA? and then in S have diffrent? Isnt all the cells are independent from the first to the kast up to the cell condetion and age?! Please answer me

@aysibrahim4739 6 жыл бұрын

Im just a new in this faild and i amost have lack of infos

@kktan3153 5 жыл бұрын

it might be because S phase is relatively long and that in this long phase, the cells are replicating their DNA at different speeds. G1 phase is also long, but they are not replicating, so the DNA content is consistent.

@aysibrahim4739 5 жыл бұрын

Manners where are you thank u