Brief tutorial with Flowjo analyzing samples of:
- GFP positive lymphocyte cells stained with:
→Anti-CD3 APC antibody
→Anti-CD45RA PE antibody
This video covers:
- Checking sample quality
- Setting gates for GFP positive lymphocytes and single cells
- Setting compensation from single stained samples
- Setting quadrants for positive and negative cells
- Creating batch reports
#flowjo #flowcytometry #compensation #gating
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