Compensation in FlowJo Jan 21
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Күн бұрынThe compensation options in cytometry have expanded significantly over the past few years. In this webinar we'll discuss the use, benefits, and interaction of AutoSpill/AutoSpread, spectral compensation, and Ye Olde compensation (naming credit to Sabine Ivinson), using FlowJo version 10.7.
@madhavsharma3362 2 жыл бұрын
good tutorial
@jacquelinehowells4796 Жыл бұрын
In autospill I saw that you removed your universal negative after adding an unstained control to an empty channel. do you have to re-assign that sample as the universal negative? If not, then how does compensation get subtracted/what would be the purpose of adding it to an empty channel? Thanks so much for this tutorial! It was super helpful.
@FlowJoMedia Жыл бұрын
Hi Jacqueline, Autospill compensation requires no negative control. An unstained cell control can optionally be added to an empty detector in order to estimate the background fluorescence present in the cells. Then when compensation is performed, the signal from the unstained control present in each other detector will be removed, subtracting out the autofluorescent signal. Learn more about Autospill compensation on our website: docs.flowjo.com/flowjo/experiment-based-platforms/plat-comp-overview/autospill-compensation/
@user-zt2vv4yc2t 10 ай бұрын
Мне обманывали мошенники
Compensation in FlowJo. Matrix Editing: An Advanced & Unique Approach in Flowcytometry data Analysis
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