Making a liquid culture without a pressure cooker

Рет қаралды 17,732

Жыл бұрын

This video explains how to make sterile media used for mycology without using an autoclave or pressure cooker! LINKS FOR EVERYTHING USED IS AT THE BOTTOM - all suggestions are from our Amazon affiliate including the exact setup that we used in this video - MUSHLOVE
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LAB GEAR USED IN VIDEO:
Media:
PEPTONE: amzn.to/3vIWsr6
Tubing:
5/16: amzn.to/3WR7yXd
3mm: amzn.to/3CqTEmv
Peristaltic Pump:
BENCH TOP: amzn.to/3WQK8kv
LAB GRADE: amzn.to/3w0jBWt
INDUSTRIAL: amzn.to/3GKo7hC
Vacuum Pump:
BENCH TOP: amzn.to/3VJWSYR
LAB GRADE: amzn.to/3VN6BxO
INDUSTRIAL: amzn.to/3CrYOyF
Media Filter Bottles:
500mL: amzn.to/3GgUBPn
1L: amzn.to/3GKj5lA
Glass Media Bottles:
500mL: amzn.to/3vJX53Y
1L: amzn.to/3GI7a7C
Replacement injection Ports: amzn.to/3QiMINS
Syringe Filters: amzn.to/3GFX2we

Пікірлер: 91

@johnkoury1116 9 күн бұрын

It is so cool how the pharmaceutical laboratory equipment and techniques are filtering down to the mycological production methods...really awesome!!

@JimmyGreen-wx6gt 9 ай бұрын

The first time I watched some of Gary's videos a couple yrs ago the words "over qualified" came to mind. The information he shares and the way he delivers it is awesome.

@alexjakson2369 Жыл бұрын

"Yer a wizard, Gary" 🧙‍♂🪄👍

@00wop80 Жыл бұрын

You don’t know how much money you just saved me. I’ve been buying mine at $15 a pop

@DuneNobleman Жыл бұрын

As always great content Gary. Looking forward to hearing more about your bioreactor project.... Mush love dude!

@skyelord6229 Жыл бұрын

I love the science you drop on us, great video!

@tartarqueen Жыл бұрын

I think I’ll just use the pressure cooker!!!!!!!

@bwandonmelon6333 Жыл бұрын

Loving all the new content dude keep up the inspiring work! You got me hooked on mycology and love your lions mane culture! 🍄 love

@adnanbulic39 Жыл бұрын

I never comment on videos, but jesus christ, thank you Gary for all the information you put out. Seriously incredible, mush love. Also definitely interested by that filtered air concept!

@FreshfromtheFarmFungi Жыл бұрын

thanks for watching and following along! Once I have it dialed in I will spread the word! So much new space and room to do ideas now Im very excited for the next few years 🙏🏻🍄❤️

@RustyColonsCPA Жыл бұрын

What!! Can't wait to see this.

@OffGridInvestor 10 ай бұрын

I worked in AI and they had one of those pumps. I believe I still have some of the silicon hose they used. Worn a bit where the pump wheels went.

@bounceurabdelaziz4973 Жыл бұрын

Hi Gary, thanks very much for your sharing,this filter is single use or reusable. if single use is what you should buy only the filter or buy the complete kit.?.

@MycoDogs Жыл бұрын

thanks man this is good info

@LarsLarsen77 Жыл бұрын

This is how they do it in industry. They sterilize the plumbing and gigantic vat (tm) with bleach water and then pump that all out and replace it with filtered media water.

@DavidBuckle-jy9nh Жыл бұрын

Don't need a pressure cooker but you do need these hard to find other gadgets

@FreshfromtheFarmFungi Жыл бұрын

yes but scalability is much easier

@flav6837 10 ай бұрын

Seems more open to contaminate really just my opinion I’m sure your good at what you do but extra hoses pumps ect lot of extra steps and u still have to open media bottle so all that just to be at same risk of standard lc methods with pc if not worst really.

@barrsgunns1751 Жыл бұрын

Do we need the rolling pump? The filter should keep contam out of finish product right?

@tonystewart7624 Жыл бұрын

Hey Gary thank you for sharing new ways to make LC. I remember how hard it was to keep my daughter asleep and my wife happy after she was born. Noise was a big complaint. Lol

@FreshfromtheFarmFungi Жыл бұрын

necessity the mother of invention 🙏🏻

@tonystewart7624 Жыл бұрын

@@FreshfromtheFarmFungi Very true brother.

@benchadwick1754 Жыл бұрын

I like your shirt.

@dario2rnr 2 ай бұрын

I thought he said "scallop" the system. I was perplexed until he said it again.

@shauncobb6 Жыл бұрын

Man it makes sooo much sense far out your the man omg Chur bro

@JoeGruszkowski 4 ай бұрын

So do i need to boil the water or anything beforehand? Ive been trying to find a way without a pressure cooker this is perfect.

@FreshfromtheFarmFungi 4 ай бұрын

no boiling required the filter will take any contaminants out 👍

@meachyofficial Жыл бұрын

Loving your videos! I'm going to start a small farm in the summer. Hopefully i can sell some.

@FreshfromtheFarmFungi Жыл бұрын

my advice is start small and build on your success 🍄❤️

@nrsrchd Жыл бұрын

If I up grade pump to the lab one you have listed, will I still need the peristaltic pump?

@FreshfromtheFarmFungi Жыл бұрын

no it is only optional you will just need to scoop the media into the top reservoir before it filters it all continuously

@NikolaNikola69 6 ай бұрын

👍👍

@Enochulate88 Жыл бұрын

Hey Gary!!!!!!

@coloradodirtbike5930 Жыл бұрын

Hey Gary happy new years, hope all is well. Quick question about glass petri dishes, do you have a specific brand you would recommend? I need to get some reusable glass petri dishes but want to make sure I buy the right ones that are built well and the lids fit good.

@FreshfromtheFarmFungi Жыл бұрын

I haven’t used them often because of the cleaning time required but these look good Ill do a video soon amzn.to/3WWBtgq

@happymaui Жыл бұрын

Selecting mycelium from agar

@tyleregan9927 Жыл бұрын

How far in advance of inoculation can you make up liquid culture?

@FreshfromtheFarmFungi Жыл бұрын

if you keep it unopened you should be able to make it indefinitely before hand. It should remain sterile

@Isiltarie Жыл бұрын

Also peristaltic pump-why is that necessary at all? That filter bottle is made such that you just have to pour everything in there at once and just turn on the vacuum.

@FreshfromtheFarmFungi Жыл бұрын

the point is to scale this

@Isiltarie Жыл бұрын

@@FreshfromtheFarmFungi so to sterilize, say 50 L using a single filter? Well yeah if it works that way that would be good.

@jordan6921 Жыл бұрын

Hey man! I’ve watched so so many of your videos and they’re all so informative!! (Your mushroom breeding videos are my favorite) I’m a cadet at USAFA right now and I’m trying to start a mycology club soon. Have you tried using food waste (and possibly wood waste) to grow mushrooms?? I have an idea on using our dining halls waste, composting it, or sterilizing it to use as spawn starter or substrate. Do you think that idea is plausible for something like Lions Mane or a more popular edible mushrooms?

@shroomieshrooms6551 Жыл бұрын

Composting woodchips with composted food waste cant work for woodloving mushrooms such as lionsmane or if it does it'll be very difficult and low yeild. Instead what you should do is compost food waste mixed with straw, grass cutting, wood shaving/chips and/or manure. This'll create a mix ideal for button mushrooms or even almond agaric and other tertiary decomposers. Composted materials is for tertiary decomposers mostly, which are your mushrooms that grow from soil or even manure, where as most gourmet mushrooms are primary decomposers and require not decomposed woodchip/substrate, although they can but wont produce the best yield and will most likely cause contam issues if not steriled properly

@FreshfromtheFarmFungi Жыл бұрын

it may work for secondary decomposers (agaricus, stropharia etc.). I have used spent grains from a distillery for spawn but it was very time sensitive. If you want to grow lions mane, look for local woodworkers who use raw hardwoods like table manufacturers or people who sell big slabs and you can grow on that relatively cheaply 👍🍄❤️

@jordan6921 Жыл бұрын

Thank you both!!!

@shauncobb6 Жыл бұрын

You could also if willing to wait use your vacuum sealer and use same setup as ya media extractor no ? Using air inlet with .1micron syringe filter scaling down....

@FreshfromtheFarmFungi Жыл бұрын

this might work as well maybe having one filter for liquid and one for air exchange as it might get clogged easily

@shauncobb6 Жыл бұрын

@Fresh from the Farm Fungi yea for sure with check valve after filter b4 jar so can just remove it when jar filled I think if it's blocking up too much then your lc messy anyways I've been using brf syrup and glucose and it's so clean to start with should give it a try im even doing .5g nutritional extract per 1L but I think peptone would be cleaner

@shauncobb6 Жыл бұрын

@Fresh from the Farm Fungi also mate your work I have been following for couple years now and thank you for your videos so much help got any ideas for mushroom mites ? Kinda hard to find much info about them and only a problem couple month of the year I am thinking maybe they have followed me from growing plants

@FreshfromtheFarmFungi Жыл бұрын

@@shauncobb6 try nematodes and ladybugs yes I am also a horticulturist and have to deal with insects - the best way is to prevent but now the battle has begun! 👍😎

@shauncobb6 Жыл бұрын

@Fresh from the Farm Fungi Chur too true thats a good idea im winning but hard to iradicate lol so springs up now and then I know in commercial situations they use piperonyl butoxide as 1day withholding

@RobertSmith-me3gs Жыл бұрын

But where can I get that shirt?

@FreshfromtheFarmFungi Жыл бұрын

I was gifted this shirt I believe it was from ETSY

@NotaFEDjed 21 күн бұрын

Why not use a syphon instead of a special pump?

@FreshfromtheFarmFungi 15 күн бұрын

@@NotaFEDjed you can use a syphon at this scale but the peristaltic pump is a closed system so it can be scaled up in a sterile environment

@themyceliumnetwork Жыл бұрын

2.5g of peptone in 500ml is a huge amount is it not? or does the filtering remove most of it? i've added 0.5g in 1,000ml of LC & my mycelium grew so fast it started growing mats in the jar after just a few days.

@FreshfromtheFarmFungi Жыл бұрын

yes it is a lot but it’s still 10x less then other recipes I use. .5% peptone compared to 4% honey water - you can get away with much less 👍

@flakey1228 Жыл бұрын

@@FreshfromtheFarmFungi Try 5% Dextrose with 0.08% soy Peptone. Anything above 0.1% slows down the growth. For 500ml 475g destilled water 25g dextrose +0.4g Soy Peptone You can also buy a slready sterilized 5% glucose solution with injection port from for example braun. There is enough air in the water, to support ~2 weeks worth of mycelium growth. A good methode is to sterilize a peptone solution and inject 0.08% soy peptone into the glucose solution.

@Tahemushroom 11 ай бұрын

❤❤❤🌹🌹🌹🌹🌹🌹🌹🌹

@johno6800 Жыл бұрын

Ill think ill just buy a pressre cooker lol

@Isiltarie Жыл бұрын

eight of those sterile cups would buy me a pressure cooker...

@FreshfromtheFarmFungi Жыл бұрын

yes but not a full scale retort autoclave which the volume can produce. It will make sense in time I am developing a whole system this is just scaled down to show on a bench top.

@Isiltarie Жыл бұрын

@@FreshfromtheFarmFungi Okay, that may make sense in that context

@dhaval4570 Жыл бұрын

Gary I didnt get what you said about the Lc lid not having a syringe filter?? Would love an explanation

@FreshfromtheFarmFungi Жыл бұрын

the white lid can have a syringe filter and injection port put in the top so they can be used like the mycology lids on our ETSY

@dhaval4570 Жыл бұрын

@@FreshfromtheFarmFungi i know that, but why didnt you have any ? Doesnt it slow down the lc growth ? I am asking because I have a few jars with syringe filters but I have to be careful while agitating them manually to avoid getting the filter wet.

@themyceliumnetwork Жыл бұрын

he is talking about the lid for the filtered media bottle lid. adding the syringe filter will allow your mycelium to breath (you will need to re-sterilise your lid though) kzfaq.info/get/bejne/b7qIdcx9rrPXpWg.html

@FreshfromtheFarmFungi Жыл бұрын

@@dhaval4570 yes I have another reason - these will go into our bio reactor setup that feeds in fresh filtered air I haven’t finished this setup yet which is why it doesn’t make sense really 🙏🏻🍄❤️

@dhaval4570 Жыл бұрын

@@FreshfromtheFarmFungi 😊 mush love gary, do share when finished 🙃.

@InconspicuousOwl Жыл бұрын

Woaaahhhhhh 🔥 🔥 💉

@BoroBootBoy Жыл бұрын

Seems more complicated than necessary. If you have to sterilise the jars you might aswell do it with the LC inside. Okay, you need to let it cool, but that's just something to plan for. Also.... Peptone.... it must be cheaper over the pond.... it's ridiculously expensive here!

@FreshfromtheFarmFungi Жыл бұрын

Yes it makes more sense at larger scales you will see once I get mine set up 👍

@flakey1228 Жыл бұрын

Why? You normally do not use more than 0.1% soy peptone. Its reallt inexpensive by the weight you need