Gel Electrophoresis Lab
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Күн бұрынTeacher Ms. Curley explains how to perform our gel elctrophoresis lab. In this lab (from Carolina Biological), students will separate DNA fragments of three "viral" samples to determine how closely related the samples are (and, perhaps, how virulent the most recent viral outbreak will be).
@nibrassbio8937 20 күн бұрын
Thank you for this wonderful video ❤❤❤I like it so much ❤❤❤❤
@tinam1415 5 жыл бұрын
10/10 would watch again. will probably show it to my class
@walterwhite4699 3 жыл бұрын
Really beautiful demonstration. Appreciate it! Thanks!
@nafisasamad3115 6 жыл бұрын
very easy and informative video, you have explained it very well, thank you ma'am, but no explanation about the addition of ladder and positive control and negative control, plz tell about these too ?
@ScienceCreAchins 6 жыл бұрын
A DNA ladder is just a standard set of DNA fragments that are various, but known, lengths (bought from a biological supply company). They will be pulled through the gel, with the smallest pulled through faster than the larger fragments. When the gel is disconnected and stained, the ladder can be compared to the unknown fragments in other wells/columns, so their lengths can be estimated. (The ladder is used to make a standard curve with semi-log paper (distance vs. length of fragment), and the unknown fragments can be estimated by using the curve made with the semi-log paper). I don’t think we actually used a ladder or controls for this lab, just the three strains of (fake) virus DNA (it's a 9th grade, intro to biotechnology lab). Here is a link to the lab directions: www.cpet.ufl.edu/wp-content/uploads/2013/10/Outbreak_virus_dna_teacher_manual.pdf
@scolary5147 5 жыл бұрын
thank you for your video and further explanation
@MrSoumyaBanerjee 3 жыл бұрын
Thank you, this was very illuminating.
@GodiyaMoses 5 жыл бұрын
this is a really informative and detailed video thank you so much
@swapnadeepd7363 2 жыл бұрын
Wonderful video.....I am from the field of Biotechnology and also in the teaching profession....I loved viewing the video😊
@swapnadeepd7363 2 жыл бұрын
Beautifully made video 😊 I myself am from the field of Biotechnology and also in the teaching profession...loved viewing the video 😊
@lamie7767 Жыл бұрын
hey! i am an aspiring biotech major and had some question that i want to ask if you have time!
@diksha8756 3 жыл бұрын
Awesome
@AuraNaturae 3 жыл бұрын
Ethidium bromide is orange dye . But here it is voilet which dye it is.
@082.apoorvanidhiacharya8 4 жыл бұрын
What are used as DNA samples???
@ScienceCreAchins 4 жыл бұрын
We used DNA from this kit: www.carolina.com/dna-gel-electrophoresis-kits/outbreak-fingerprinting-virus-dna-8-station-kit-dna-only/211204.pr
@ramkeshsaini9345 5 жыл бұрын
Tell we CDNA library
@ramkeshsaini9345 5 жыл бұрын
How to attach DNA in vacter
@vineetpandey5309 2 жыл бұрын
Probably nice video.
@BarbacoaAppliances818 5 жыл бұрын
Those googles though, lol I bet her eyes were boiling from steam and pressure lol
@LarsLarsen77 Жыл бұрын
Strand? LOL
@sunitaagnel-3629 3 күн бұрын
May be Bromophenol blue
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