#PSB#Photosynthetic bacteria#how to do psb Photosynthetic bacteria or PSB are anaerobic bacteria that perform photosynthesis. Now I decide to find some myself without buying them directly.
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@thetruthserum28164 ай бұрын
Thioglycollate broth is a microbial culture medium that contains thioglycolic acid. Thioglycolic acid is a reducing agent that creates an oxygen-depleted environment that promotes the growth of anaerobic bacteria. The medium also contains resazurin, a redox indicator that changes color in response to oxygen levels. Thioglycolic acid is in hair remover.
@SanctuaryGardenLiving11 ай бұрын
I think you can take the whole column and put it in the freezer then you can remove specific samples from the different layers.
@ckmbyrnes4 ай бұрын
And it will not damage the sample?
@SanctuaryGardenLiving4 ай бұрын
@@ckmbyrnes not sure, just an idea. Depends on the material of the column. I'm sure it would effect the living organisms however it may be a good tool to study specific layers.
@juanbait96703 ай бұрын
@@ckmbyrnes this sort of bacteria most likely can survive high/low temps, it just goes dormant untill conditions are right.
awesome! how may i use the psb in agriculture ? i heared some1 respectable tellin me its good stuff but its been hard for me to find anything understandable x) your video helped a lot tho
@shimpiden2 жыл бұрын
Thank you for sharing this video, looking forward to your followup video on how to more easily remove the red bacteria.
@diymicrobiology3782 жыл бұрын
Thank you, I am trying that right now.
@kompasperubahan7968 Жыл бұрын
@@diymicrobiology378 hi, any updates about this? your videos are incredible
@atitudesqueaproximam25273 ай бұрын
pabens pelo vídeos e por reponder as perguntas!
@andrewgordon60922 жыл бұрын
Thank you for the video. Is the red bacteria in your PSB Rhodobacter? I was told that it is, but then I read that Rhodobacter is a non sulphur one. Many Thanks!
@diymicrobiology3782 жыл бұрын
Most likely to be chromatium spp.
@andrewgordon60922 жыл бұрын
@@diymicrobiology378 Thank you! I am based in Thailand and have been dabbling with the PSB for the past 6-7 months. Lots of people here use the egg + msg + fish sauce receipe. I tried it with mixed results. If you are interested to know more about my results/failures please drop me your email. Thank you!
@aherrns1894 Жыл бұрын
@@andrewgordon6092 hi! Care to update? Pls share your email adress.
@erbauungstutztaufgnade18755 ай бұрын
cool God bless
@vuhavi7985 Жыл бұрын
Hi, thanks for the great video. May I ask the recipe for the refined medium?
@diymicrobiology378 Жыл бұрын
It is made from egg, MSG, and soy sauce.
@laprankster32644 ай бұрын
I’m sure this process can be used with marine photosynthetic bacteria and not just freshwater?
@diymicrobiology3784 ай бұрын
Yes, it can be use with sea water, too.
@laprankster32644 ай бұрын
@@diymicrobiology378 Cool.
@eathanehanthnaidu11a406 ай бұрын
Which part of it produces the most amount of oxygen?
@diymicrobiology3786 ай бұрын
Since most of them don't even produce oxygen, the very top green layer makes the most oxygen.
@lajuramesh64475 ай бұрын
Why boiled egg used in the medium
@diymicrobiology3785 ай бұрын
I don't know what kind of egg to use at first so I used boiled egg.
@yulianasutikno2 жыл бұрын
Thanks for this informative video. What would be the minimum temperature for these bacteria to grow and can they grow under full spectrum grow light instead of direct sunlight?
@diymicrobiology3782 жыл бұрын
They can grow in room temp. You can grow them using growth light or just light bulb.
@yulianasutikno2 жыл бұрын
Many people are using purple bacteria cultivated by eggs+fish sauce+pond water method. Does this method produce most likely PSB or PNSB? They are using the bacteria for agricultural application, but I am not sure if the media (protein, egg, soy sauce) is actually causing a better growth compare to just plain water.
@diymicrobiology3782 жыл бұрын
The eggs+ fish sauce+ MSG method will result in sulfur bacteria, that is the reason to put the egg in(to provide the element S) The growth come from the bacteria, not the medium, for it’s effectiveness, you have to try on your plant to find out.
@yulianasutikno2 жыл бұрын
@@diymicrobiology378 Thank you. Sorry I meant the better growth of plants treated with PSB (compare to just plain water). Is there a way to isolate PNSB without complicated lab equipments? Sorry for asking a lot, you are very knowledgable, therefore I am asking this. Thank you once again.
@diymicrobiology3782 жыл бұрын
As far as I know, there are no simple ways to isolate non-sulfur bacteria, they do not grow in bloom like sulfur bacteria. You have to at least have the chemicals for medium and anaerobic culturing equipment.
@watchingaccount6622 жыл бұрын
How did you take the puple part of those layers in a jar?
@diymicrobiology3782 жыл бұрын
I use a long glass straw to suck the bacteria from the layer I want.
@reefonbux9432 Жыл бұрын
@@diymicrobiology378 What?! Pause.
@Dave_The_Beatmaker2 жыл бұрын
@5:08 How did you isolate the red layer? Its almost at the bottom of the container. And how did you grow it to get this beautiful big bottle of red sulphur bacteria? Thanks
@diymicrobiology3782 жыл бұрын
It would be difficult to get the bacteria with tall bottle, I use a long glass straw to suck the bacteria from the layer I want, and put them into medium made with egg. But it wont be easy, you might have to try several times to work.
@Dave_The_Beatmaker2 жыл бұрын
@@diymicrobiology378 Thank you so much for your answer. suck out the red layer? LOL what a great idea. But how did you avoid the bacteria entering your mouth? What would be a better container?
@diymicrobiology3782 жыл бұрын
@@Dave_The_Beatmaker Don't use your mouth!! use a syringe or something. I am still trying a better container, I will make a video if I am success.
@Dave_The_Beatmaker2 жыл бұрын
@@diymicrobiology378 UPDATE: SUCCESS! In the beginning nothing happened. But it was still cold at night and maybe thats why nothing happened at first. I used water from a local river. 90% of both glass jars have GREEN PSB with some red spots. But mostly Green PSB. Furthermore I created PSB by using EGG + MSG + SHRIMP. I used stored rain water. ALL containers turned green. Do you know why ?
@diymicrobiology3782 жыл бұрын
@@Dave_The_Beatmaker It can take month to grow the bacteria, river water might have lower purple PSB since it is running water. The green one you get from the rainwater might because of the algae grow inside. Check the water under a microscope if you have one. Rainwater normally do not have PSB since it has a lot of oxygen.
@pillowwantan32262 жыл бұрын
What can we use instead of MSG?? Something easy to make/find, if MSG is unavailable?
@diymicrobiology3782 жыл бұрын
As far as I know, any short chain organic carbon will do, like malic acid, pyruvic acid, succinate acid…etc. You can also try sodium carbonate.
@pillowwantan32262 жыл бұрын
@@diymicrobiology378 sorry, I'm not a scientist of any sort, so any of these just confuse me .. I have some lactic acid bacteria currently fermenting??? Some rice vinegar, some lemon juice, not sure if any of these could work???
@diymicrobiology3782 жыл бұрын
@@pillowwantan3226 Do you have any artificial seasoning? Aspartame? or urea?
@pillowwantan32262 жыл бұрын
@@diymicrobiology378 no, nothing like that! I prefer to use fish sauce, sesame oil, soy sauce and rice vinegar and any sort of herbs. Just trying to stay away powders and such
@diymicrobiology3782 жыл бұрын
@@pillowwantan3226 Then I guess you will have to cook some mashed mushroom and kelp stew into super thick soup, thought I don’t know will it work or not.
@SG_Tony11 ай бұрын
英文講解優👍
@vishwanathshashidhar33742 жыл бұрын
Sir how to remove red color
@diymicrobiology3782 жыл бұрын
What do you mean? The red color is from the bacteria itself.
@afkarezky Жыл бұрын
hi, can this purple sulfur bacteria fix N2? and how do you use them in plant?
@diymicrobiology378 Жыл бұрын
Yes they can fix nitrogen. You can further culture them and add them into soil. But if you want nitrogen fixation there are bacteria more efficient.
@afkarezky Жыл бұрын
@@diymicrobiology378 what bacteria is that? is it easier to culture than PSB?
@diymicrobiology378 Жыл бұрын
@@afkarezky something like bacillus or klebsiella, much easier to culture than PSB.
@afkarezky Жыл бұрын
@@diymicrobiology378 thank you for the information
@reefonbux9432 Жыл бұрын
Can you sell me that lol?
@diymicrobiology378 Жыл бұрын
If you mean the bacteria, no. I don't have the equipment to store and transport bacteria for long term, also the purpose of this video is not selling bacteria.