And it will not damage the sample?

@@ckmbyrnes not sure, just an idea. Depends on the material of the column. I'm sure it would effect the living organisms however it may be a good tool to study specific layers.

​@@ckmbyrnes this sort of bacteria most likely can survive high/low temps, it just goes dormant untill conditions are right.

Thank you.

Thank you, I am trying that right now.

@@diymicrobiology378 hi, any updates about this? your videos are incredible

Most likely to be chromatium spp.

@@diymicrobiology378 Thank you! I am based in Thailand and have been dabbling with the PSB for the past 6-7 months. Lots of people here use the egg + msg + fish sauce receipe. I tried it with mixed results. If you are interested to know more about my results/failures please drop me your email. Thank you!

@@andrewgordon6092 hi! Care to update? Pls share your email adress.

It is made from egg, MSG, and soy sauce.

Yes, it can be use with sea water, too.

@@diymicrobiology378 Cool.

Since most of them don't even produce oxygen, the very top green layer makes the most oxygen.

I don't know what kind of egg to use at first so I used boiled egg.

They can grow in room temp. You can grow them using growth light or just light bulb.

Many people are using purple bacteria cultivated by eggs+fish sauce+pond water method. Does this method produce most likely PSB or PNSB? They are using the bacteria for agricultural application, but I am not sure if the media (protein, egg, soy sauce) is actually causing a better growth compare to just plain water.

The eggs+ fish sauce+ MSG method will result in sulfur bacteria, that is the reason to put the egg in(to provide the element S) The growth come from the bacteria, not the medium, for it’s effectiveness, you have to try on your plant to find out.

@@diymicrobiology378 Thank you. Sorry I meant the better growth of plants treated with PSB (compare to just plain water). Is there a way to isolate PNSB without complicated lab equipments? Sorry for asking a lot, you are very knowledgable, therefore I am asking this. Thank you once again.

As far as I know, there are no simple ways to isolate non-sulfur bacteria, they do not grow in bloom like sulfur bacteria. You have to at least have the chemicals for medium and anaerobic culturing equipment.

I use a long glass straw to suck the bacteria from the layer I want.

@@diymicrobiology378 What?! Pause.

It would be difficult to get the bacteria with tall bottle, I use a long glass straw to suck the bacteria from the layer I want, and put them into medium made with egg. But it wont be easy, you might have to try several times to work.

@@diymicrobiology378 Thank you so much for your answer. suck out the red layer? LOL what a great idea. But how did you avoid the bacteria entering your mouth? What would be a better container?

@@Dave_The_Beatmaker Don't use your mouth!! use a syringe or something. I am still trying a better container, I will make a video if I am success.

@@diymicrobiology378 UPDATE: SUCCESS! In the beginning nothing happened. But it was still cold at night and maybe thats why nothing happened at first. I used water from a local river. 90% of both glass jars have GREEN PSB with some red spots. But mostly Green PSB. Furthermore I created PSB by using EGG + MSG + SHRIMP. I used stored rain water. ALL containers turned green. Do you know why ?

@@Dave_The_Beatmaker It can take month to grow the bacteria, river water might have lower purple PSB since it is running water. The green one you get from the rainwater might because of the algae grow inside. Check the water under a microscope if you have one. Rainwater normally do not have PSB since it has a lot of oxygen.

As far as I know, any short chain organic carbon will do, like malic acid, pyruvic acid, succinate acid…etc. You can also try sodium carbonate.

@@diymicrobiology378 sorry, I'm not a scientist of any sort, so any of these just confuse me .. I have some lactic acid bacteria currently fermenting??? Some rice vinegar, some lemon juice, not sure if any of these could work???

@@pillowwantan3226 Do you have any artificial seasoning? Aspartame? or urea?

@@diymicrobiology378 no, nothing like that! I prefer to use fish sauce, sesame oil, soy sauce and rice vinegar and any sort of herbs. Just trying to stay away powders and such

@@pillowwantan3226 Then I guess you will have to cook some mashed mushroom and kelp stew into super thick soup, thought I don’t know will it work or not.

What do you mean? The red color is from the bacteria itself.

Yes they can fix nitrogen. You can further culture them and add them into soil. But if you want nitrogen fixation there are bacteria more efficient.

@@diymicrobiology378 what bacteria is that? is it easier to culture than PSB?

@@afkarezky something like bacillus or klebsiella, much easier to culture than PSB.

@@diymicrobiology378 thank you for the information

If you mean the bacteria, no. I don't have the equipment to store and transport bacteria for long term, also the purpose of this video is not selling bacteria.