"You're one to talk! Mr came from egg juice"

Sounds an awful lot like someone out of blue would say.

well at least that makes more sense then the racism we have today!

such a vitamin water thing to say 🙄🙄🙄🙄

More like dark bear or white bear.

A new and more sustainable form of mad science.

I should not have read that while eating b/c I nearly spat out my food cackling 😂

He makin zombies for sure

you've created him ! Is he Doctor Frankenstein or the Frankenstein Monster ?

I'm pretty sure sugar is already included.

And then the perfect little girl was born

@@darthplagueis13I can’t imagine being satisfied with the sweetness of a Gatorade, tbf. they’d be majority spice and chemicals which is something but isn’t the ratio to be power puff girly enough (according to my own conjecture let’s be real for a sec lmfao-)

@@rose.isnotavailableerm huh uh ok 🫤 🤔 🙄 👍

we're trying to make something to eat, not give it the right to vote

Huh... I don't what you're saying but I kinda wish I understood more of what you are talking about now

@@codiserville593 I think they're saying that we could get the cells to evolve so they could survive in pure gatorade

They should definitely try and see if there’s a way to fully transition a cell line to a cheaper medium.

@@jacobwiens659 For sure. The cost involved in obtaining the growth media are one of the biggest obstacles to bringing down the cost of production. (The cost of electricity is another, but we already have a lot of information on how to solve that)

@@goo894 well alright thanks for this possible translation. It's helpful

You can bring red gatoraid to work, dilute and have some left over for your own mad scientist creations!

Also...Our budgets are not unlimited((((

no sir tax-man these energy drinks are 100% for Science!

Monster Energy meat next

@@calamatica To be fair, it's not the labs that think the budgets are unlimited, it's the purveyors of the materials used. :-(

Yeah, it’s just “how much green dekara and coconut water can you put in a Petri dish before cells notice?”

although this is the opposite of the sawdust in a way, because adding more Green dakara makes it more tasty

A very bad analogy. This has nothing to do with thinning out a product, it's a new way of producing meat without killing animals.

@@skunkjobb I wasn't making an analogy I was making a goofy comparision

@@skunkjobb It's _LITERALLY_ thinning out a product. Did you not catch that they are using the Gatorade and other drinks as partial replacement for the cell media? Or all that various other stuff as partial or complete replacement for the serum?

Not entirely. There's almost always going to be minor or major compromises. You might be able to filter or separate out some of the undesirable elements, but every bit of that effort eats into the cost/time savings of using the retail alternative in the first place. But if something along that cheaper price point spectrum works "good enough" with 99.8% or whatever effective substitution, then yeah. For example, Thought Emporium mentioned two differences: the sports drinks significantly dropped pH balance out of the optimal cell survival range, and some trace elements in DMEM are situationally important for stuff like growing bones. I'm no cell biologist, but I'd imagine there are more nuances like these that they didn't mention. Another big one is quality control and precision to prevent variation. Two units of the same sports drink from different bottling plants or batches in the same plant could have a huge 0.2% variation in concentration of some elements. Not enough for a consumer to taste more or less salty, but could be enough to throw a precise lab process out of whack. A lot of the extra $100s of cost is for processes that ensure these error margins are orders of magnitude smaller. So the discount sports drink doping might be good enough for growing boneless McRibs or chicken nugget paste, but not for pseudo pork chops or beef ribeyes that need more realism. Good budget tier alternative for producing off-brand or generic synthetic meat, while the top quality synthetic meat will cost 2X more just to add the necessary 0.2% trace elements or whatever. Just like current consumer product price tiers. Or the discount stuff could work great for pumping out lower cost protein feedstock to feed to actual animal livestock that's raised for real meat. Not as environmentally or cost optimal as directly growing a high quality vat steak, but maybe it would be an interim technology that cuts cost inputs and greenhouse gas emissions by 10% or something, per pound of real beef produced. That's basically what industrial agriculture has been doing at a lower tech level for the past century -- grinding up undesired wild caught fish bycatch and waste parts from farmed fish to make fish meal, which they then mix into feedstock for more farmed fish or other livestock. Same for waste parts from butchered cows, pigs, chickens, etc that are fed back into the system to feed new animals. (and yes, this has been a source of increased system vulnerability to contamination, disease, and things like growth hormones recirculating back into the system as it approaches both higher efficiency and a slightly more closed loop of production)

Lab grown meat ..? Dont you mean DIY grown meat ? 😁😆

The reasoning is because those expensive mediums are specifically tested and the ratio of contents confirmed to not have anything additional in there to not throw off experiments. It’s the same reason anything “aircraft grade” is expensive. It’s also the same reason NIST sells peanut butter for like $400. You’re not paying for the item but for the paper trail to validate your results. This is especially important in healthcare research in the case of any kind of side effects or other issues later on. Stuff like this is definitely great to know, especially if you’re just doing some preliminary research into a topic, but if you’re publishing a paper then you should be using standardised ingredients/processes.

you can't make me eat it

Wouldn't be the first time something like that happened. A while back a lab tested using the old Shrinky Dinks toy to create patterns for use in microfluidics. It worked.

I mean-

just made me think of Dracula biting someone's booba . lady " help Dracula keeps biting my nips "

The medieval alchemists would be proud!

Let's not crush this one with a book

homunculus is easy, though. Test tube babies already exist.

That would be a fucking insane and I want to see it

​@@FleshWizard69420 If it behaves, and doesn't spit acid at its creator.

can't wait to have a gamer burger one day

Gamer nuggets

I made a gamer grape????

Ew

Perfect for the Doom Brain Bot

Thought Emporium is the mad scientist the world needs more of.

honestly the high cost seems to be one of the big barriers to practical lab grown meat, not to mention any other lab grown cells, so pretty useful

@@porteal8986 Exactly. An imagine being able to supply all your meat at the cost of some gatorade and an egg (so about $2 instead of the normal $15)

@@NoAIStudios we re about 50 years away from every home having their own meat synthesizer in the basement that you just need to fill the vats with gatorade and eggs every month

This is amazing and absolutely what it should be called.

Clicked on this video expecting the Idiocracy references...did not disappoint

The plan was always to call it "brawndo: the cell growth mutilator, custard flavored"

@@thethoughtemporium Perfect, let me know if you want me to design the shirt :)

​@@thethoughtemporium the neuron optimized version can be Braindo

"No John, your not allowed to be making meat out of Gatorade in the garage"

Because KZfaq makes more money off of them, so of course they allow it.They are both scammers after all.

So true

ultimate gamer brain

I'm gonna guess "as soon as we figure out how to make it actually work" In fact, i would assume there's already at least some footage recorded of testing and such

If you grow it in Gfuel it will be esports ready.

@@AageV PERFECT

It's got electrolytes! It's got what brains crave for.

Top ten cursed phrases of all time.

A blender does the same

Don't the blades kill and cut the cells?

holy shit

@@arge7661 It doesn't. Blender breaks the cells and make it incredibly messy barely soluble and un-cleanable paste. Way more harm than good.

Such a shame. But it is the world we live in, and money seems to be the best tool we could've come up with. It's both one of my favorite and most hated inventions, haha.

Yeah it can be a pain, my advisor basically doesn't have free time with the constant grant applications he has to file, granted it is a fairly large lab

Me rn in Singapore chugging the $1.50 (usd1) bottle of PS

Also yes I wanted to mention they come in power form. I remember being in police cadet corps and our outfield ration included the powder. We mixed it into our water bottles that were brewing in the hot sun, and chugged it - horrible but delicious. Also had to make lunch using some cheap off-brand instant noodles, and I think some folks forgot to pour out water into the mestin for the noodles before mixing the PS powder. But anyways we opened a tin of sardines in tomato and threw that into the mestin of noodles and honestly, pretty good!

I wondered why he imported it when I can get a bottle of pocari from a local asian store in London for a few pounds. I'd be surprised if you couldn't also in Canada

@@theendoftheworldhasbeenqui2485 honestly it was probably just because its the best way to make sure you get the exact same product. yes its almost guaranteed to be the same locally but it might not be. Also i wonder if the powder might not be better because you could adjust the concentration if need be

@@ztheo2280 In hindsight (yay, I can see clearly now!) the powder form probably would have been better. Still... next cycle?

Next project turning lead to gold

@@adengoldstein5130 ...using mushrooms and baby cow juice

@@adengoldstein5130 wikipedia/Golden_rain_demonstration

If they whip out a large metal armor, we better be very worried

Next project combining a dog and a little girl

That's amazing

Hopefully it can be used for all sorts of cell culturing applications beyond the private sector... And that the recipe and protocol is open source

so a pressure cook it to a paste then feed it to vegans, sounds like the pink sludge in fallout

@@Blimsky I am a vegan, and I approve of this message.

Not really open source, those brands are still proprietary. But cool af

DNA? Wassat

Nah that's a good point. If DMEM can be diluted with distilled water and still work well, that's an instant drop in costs cuz every lab has access to distilled water. Heck, AC units make it as a side effect of cooling the air

Actually I think your entirely right! I hope he responds to this comment

Could be, but the 100% version with replaced fbs does remediate the results somewhat, Although yeah the data is better with dmem 0-100% with reverse osmosis distilled water dilution, it's not that complicated to do too, However the research from japan in video is probably more complete,

If my bio knowledge hasn't failed me, I think that's a negative control: a group that doesn't receive treatment and isn't expected to produce a result.

​@@jetison333you're*

That's because they reproduce every 30 minutes, they are crazy efficient.

Yep from what I've seen is since they reproduce so fast, they can quickly see any effects, changes & then go from there.. But I'm no certified scientist

there are a plethora of different strains, some of which naturally live in the human digestive system. Only a handful are harmful to people.

@@am529 I know I'm more so just commenting on how E.coli is used far more in biochemistry than anyone not in the know would ever expect. I for one didn't know until I stumbled on this channel just how much it's used in this field of work.

They're just overall pretty chill dudes. Very relaxed growth requirements, readily uptake DNA if zapped and/or sauna-ed a bit, don't stink to high heaven, they even come in nonopthogenic!

Prep for albumen print is basically what was done here. Take albumen, whisk till stiff, leave overnight, and filter out solids, 🤷🏾

@@rahulsharmajammu Ah, glair! Seems to be pretty useful stuff for more than just illuminating manuscripts and gluing books.

Much like your average dad over 40, cells just love beer

it's what secretly keeps humanity alive for millennia

I mean historically speaking wine, beer, and spirits are some of our oldest inventions.

It's basically yeast broth, especially since the way they used it massively dilutes the alcohol.

Brewing beer is cell culture. The main difference is that the spent media is the desired product.

yeah acetate ion in humans is toxic so i cant imagine having a bunch of it floating around is good for the cells

Now i want to see what happens if i soda streamed some milk xD

@@user-fz1nu9uy6q You'd get lots of foam, and coagulated proteins separating from whey. More importantly, I'm looking forward for your taste report, which is the, erm.. more interesting part 🙃

Dilute sodium acetate is extremely benign. It's used in blood transfusions and neonatal care, so it's probably close to harmless to cells.

Could use dilute HCL. Make some more salt.

So are tears.

What? That's ridiculous. It's nutritionally fortified sweat!

Don't think about the circumstances for the cows to produce milk then.

Milk is just fancy sweat. Much more tasty than normal sweat though, luckily.

​@@feuerling How DOES normal sweat taste?

Lol

A little?

​@@thehuntermikipl1170 just dilute the powder in your medium, the powder is way stronger than whatever they put in the bottle

@@_Circus_Clapped_ don't troll

that'd the make final mix 91% gatorade, and it still works well, you could probably get away with homeopathic concentrations of medium at that rate

@@chyza2012 Homeopathy is a profitable business, expanding to grow mediums.

That's 100% it, they pay to remove any variation factors. Same goes for most lab stuff.

Have no clue but tend to agree. Not required for exploratory stuff like on this channel though :)

Not sure who came up with removing all variations in order to 'baseline' ; it's a monocultural bland paradigm and actually eliminates any spice which extends life and expands consciousness. We need to understand and implement the interaction between rosemary, cell growth and antibacterial properties for instance as this among others can play a vital part in organic growth overall

​@@jonhinman2471 Removing variation is the only way to be sure your results are the the product of the experiment and not some outside force. I've never seen anyone argue against that. Then in the experiment you can make as many/much variations as you want.

@@jonhinman2471 It's just meant for running experiments, that's not meant to be a final product.

The cost might get reduced, but the market price will stay the same. That's how it usually works.

Nobody wants lab grown meat

People have already figured that out and started investing serious money. Now people are debating the Morality of it.

​@@chriswheeler6092 raising and killing a cow is a lot more immoral than growing meat. One requires death and suffering the other doesn't, and no I'm not a vegan.

@@chriswheeler6092let’s just hope it’s not harmful. Imagine a world where only the rich can afford natural healthy meat while the poor get sick with no other option

Not everyone has a local Asian market. I know my town sure doesn’t, and it’s the second biggest in the state I’m in. The most Asian thing I can buy here is like… lo mein noodles from an actual Asian restaurant and offbrand pockys from Trader Joe’s

Thing is, Thought Emporium is a Canadian channel, and outside of major cities it can be very difficult to find ethnic markets that sell stuff like that. Even within the shops that do, it can be difficult. I know here in Manitoba, there's a store in Winnipeg called Oomomo, it specialises in Japanese products. I have not once seen Pocari Sweat or Dakari Green there, but even if i had the stock changes with whats popular in Japan. but Japanese products in general can be hard to locate here. Most Asian markets i've seen cater more towards Chinese and Filipino products as we just have a much larger population of Chinese and Filipino immigrants than we do Japanese.

​@@solofdragons6446inside London, Ontario, there are a couple of Asian Markets, and I'm decently sure they have those there, if he lives nearby or wants to make the trip, it's a good idea to check.

@@solofdragons6446He’s in Montréal, Pocari Sweat is 100% available here on some japanese/korean supermarkets

Maybe the industry should move away from proprietary formulas to standardized formulas

@@kokofan50 as the video says, more or less each DMEM contain the same ingredients however such small differences may create certain issues. The point here is the homemade recipes will complicate the issue even more and academia will probably won't like it.

Hell yeah, let's break those price gouging monopolies and cartels that make research unnecessarily expensive!!! Also, I agree small differences in cell culture media do cause issues. But in a hypothetical situation where labs are making their own DMEM from the same recipe, I believe from my experiences in cell culture that variability that already exists due to differences between lots of FBS is way more significant than the likely differences between homemade batches of DMEM substitute. I hope that sentence made sense.

you should probably throw those tissues away

elsewhere in the world, someone's working on a droid army

@@adora_was_taken that’s my buddy vin, I sure hope we don’t have conflict in the future

fetal*

​@@azertyQthe cat cows are taking over

MooYow

@@azertyQ Wow. That's hilarious, how did that get in there? I'm keeping it!

Yoga serum, but only the one pose.

I was concentrating on the concentrated concentrate too

You can use coconut milk a blood plasma substitute in a pinch

At that moment just go for pure additives. Electrolyte drinks are mostly salt, sugar and flavour, sometimes with a small amount of potassium chloride

@@brylozketrzyn Proper rehydration formulas often contain BCAAs and other amino acids as well as

@@Aqoric more complex ones for sure, but we can get aminoacids from many sources. It is about balancing nutrients and hormones.

@@brylozketrzyn Absolutely, my original point was simply for large scale production it would likely prove cheaper

blud is bluffing hard with that grammar, he did not work in a chemical plant yall.

@@StoneBox_761a He was just an operator making the shine, the rest of the gang was doing science on improving the taste to sell it for more.

@@LordDragox412 regardless, i dont think he worked there with that grammar, the requirements to be there are immense.

@@StoneBox_761a He used to have perfect grammar, but then he drank some heads. That's why he's regretful he didn't study chemistry in middle school. Quite a sad story.

I think it's the kiwi curse. Even jello doesn't work with kiwi.

I don't think that will ever fly because it's not just the "affordable kit" that's involved in this. It's also all the equipment required and the sterilization and handling protocols required to grow meat tissue in labs. This team is trained in that area, and even then they're prone to mistakes because that's how it goes. So letting the average Joe meddle with this sounds like a good recipe for an outburst of salmonella or something like that. I'm not a chemist or a biologist, but with my experience in IT and programming I can attest: the hardest thing to do is making your process user-proof. Someone *will* find a way to screw things over, and in the case of foods, the responsibility falls back on the distribution company.

Wait, let me guess: The states trying to ban this are also full of right-wing, book-burning, cucumbers?

@@sparking023 Off the top of my head, I'd ask if you knew anyone who ever sued hasbro for their kid getting food poisoning from an Easy-Bake Oven cake. Or, alternatively, sued betty crocker after using unrelated salmonella-infected eggs in a cupcake batter and let their kid lick the spoon. It seems to me that whatever products would be made of a kit like this would fall under the same kind of legal classifications. As long as the obvious, instructed, and prescribed usage of the kit and its supplementary products is safe, user mishandling would be seen as exactly that, and not tie back to the kit distributor.

⁠@@sparking023tbf (a small amount of) people (non-commercially) can their own food which also requires massive precaution in handling It wouldn’t be in supermarkets but I could see a world where it becomes a culinary niche as opposed to a home lab niche

Ban the sale? Just how much did the farming lobby -bribe- _ask nicely_ for that?

Orchid, organ, potato pataya

@fisrtnamelastname3083 well growing orchids from seed is akin to cell culture due to how orchids have evolved. The seeds don't have an endosperm (they are more akin to spores than seeds) and rely on mycorrhizal fungi to provide the developing seedling with nutrients. When growing them, you have to put them in a medium to recreate the conditions. As a result, you need to work aseptic!

@@mrslinkydragon9910 wow! That's really cool! Never thought I would learn such a fact by making an awful joke lol

@fisrtnamelastname3083 oh I didn't get the joke, I just like saying facts

Yeast is fungus, and was tested. An actual mushroom would be too hard to liquify.

@@Br3ttM Yes, yeast is fungus, but it's single cell organism. In my mind multi cell organism would have more need for things requiring the growth of cells. It's true that mushrooms are not liquid and that might be a problem as they are living beings on their own 🤔 liquid cultures are a thing though. I'm not a biologist or a chemist so I can't comment if mushrooms are useful. Even milk has bacteria if it isn't pasturized.