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Hemoglobin A1c test is used to estimate glucose levels in the blood and to assess the condition of the patient whether diabetic or pre-diabetic. The test is based on the reaction of glucose on amine group of valine in N-terminal of Hemoglobin type A1c to form glycated hemoglobins. These glycated hemoglobins have average life span of 3 months there by indicates the estimated glucose levels as the average of past 2-3 months in the patient.