DNA Microarray (DNA chip) technique

Рет қаралды 328,275

Күн бұрын

Hey Friends,
DNA Microarrays cover a lot of tasks such as gene expression analysis and genotyping. How this DNA chip technique works, is explained in this animation.
Best,
Henrik
Time Codes:
0:00 Introduction: Why to use a DNA microarray
0:33 Sample preparation
1:15 DNA Microarray chip - Mechanism of Action
3:01 In the lab

Пікірлер: 107

@henrikslab 8 ай бұрын

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@simafarokhnejad5882 6 ай бұрын

This video is one of the best explanations of DNA Microarray I've come across on KZfaq. It's concise and efficient, and it is clear even for someone without a background in biology.

@V_MA 2 жыл бұрын

Great! I needed to watch a video about DNA microarray to understand how it works. It helped me a lot! Thank you.

@jadon5798 6 ай бұрын

geiler typ man hört den akzent aber du sprichst deutlich und das was du sagst ist sehr verständlich und top erklärt vielen Dank du hast mir den arsch gerettet

@monkey6493 Жыл бұрын

Better job than my teacher ever could, thanks a lot.

@roshan3190 Жыл бұрын

Exactly 💯😂

@ii.noorii Ай бұрын

One of the best videos to explain the DNA microarray, thank you❤❤

@henrikslab Жыл бұрын

From now on you can become a member of this channel! You will have access to some cool emojis like this:. This is of course voluntary, but the financial support helps me a lot.

@pitkamatka 2 жыл бұрын

Hi Henrik! I just discovered your channel and I think your videos are of great help for me. Thanks for making them :)

@Maria-jl5is Жыл бұрын

that was brief and so useful, thank you so much

@JulianTheHunter 2 жыл бұрын

So simple erklärt, herrlich! Vielen Dank

@sinamt2982 Ай бұрын

Du erklärst richtig gut, danke dir!!

@sahanahn19 2 жыл бұрын

It would have been very helpful if you could upload the notes of this microarray.. Explanation is amazing sir!!

@eloisub902 2 жыл бұрын

Really straightforward! TYSM

@muffinfighter3680 Жыл бұрын

thanks! Now, I understand how this analysis works

@yuqingcai3447 2 жыл бұрын

This is very helpful! Thank you!

@papridas8979 2 жыл бұрын

Thank you🙏 very clear explaination!

@bhavik3201 8 ай бұрын

absolutely outstanding explanation. Thank you. Love from india

@m2-instruments Жыл бұрын

Great video, very well explained 👍🏼

@graemelaubach3106 2 жыл бұрын

Awesome channel, keep it up!!!

@sameloliveira6169 7 ай бұрын

Great explanation, thank you very much

@ma.8805 2 жыл бұрын

you are the best, thank you

@callmebonobononim Жыл бұрын

You are amazing!! Now I can understand finally🎉🎉 thank you soooo much Have a nice day😆😆

@user-ch7xw4ed8u 4 ай бұрын

Amazing! Best one on KZfaq! Thanks

@warwar5380 9 ай бұрын

thank for making complicate topic easier to understand

@tina9753 Жыл бұрын

Very helpful video:) thanks!

@chickensandos 2 ай бұрын

Great video man

@shiweanyswami7371 Жыл бұрын

Thank you it just blessing to me cleared everything thank u so much please upload more vedios am enjoying to study from here and my concepts get more strong cause you made it clear thanks onece again

@khadeejazardawy971 2 жыл бұрын

thank you so much you made it easy to understand

@Mrzaharna 2 жыл бұрын

Actually it helped me a lot to understand , thanks a lot

@narthakiyasendra2917 2 жыл бұрын

Amazing job

@ebircsbus6643 Жыл бұрын

I can hear your german accent a mile away lmao This was a amazing explanation bro thx for putting in so much work!

@halimish 5 ай бұрын

that was really helpful thank you⭐️

@itstimetostudy397 Жыл бұрын

Very nice, thanks 👍

@thefayadbrothers1170 2 жыл бұрын

Very clear thank you!

@melikaabdorrahimi3768 2 жыл бұрын

Amazing!

@AM-hp7ht Жыл бұрын

Great , Good job 😍😍😍🤩🥰

@user-sz5it4wm9f 2 жыл бұрын

A good explanation 👍👍

@harekrishnamandal1428 2 жыл бұрын

Highly conceptional

@robin2385 2 жыл бұрын

well explained video, the best i habe found, but i have a question: so the oligonucleotides must be complementary to the cDNA and the cDNA has the same direcetion as the leading DNA (3' 5'), so the oligonucleotides have the complementary direction of 5' 3'? sorry for the bad english

@syedmohammadaanasfarukh890 2 жыл бұрын

Great stuff

@suriyaprakash2252 11 ай бұрын

This is very helpful.,! Thank you! I want to know about which Bioinformatics tool has been used To (Raw)data analysis.,

@tatianaasi3315 2 жыл бұрын

Very great explanation, im studying for med school entry

@user-yehia 9 ай бұрын

Great explanation

@dieterandrich1014 2 жыл бұрын

very nice very simple

@bh5169 2 жыл бұрын

Thank you!

@SajidAli-zm8fc 2 жыл бұрын

Really nice

@afnanashraf5 8 ай бұрын

Great!

@mariociencia12 3 жыл бұрын

It is incredible your ability to do concise videos with a lot of information and completely animated! I have just a question: how exactly does the cDNA become fluorescent? All the bases used are fluorescent?

@henrikslab 3 жыл бұрын

Thanks, that means a lot! I did some more in depth research... and there are many ways in practice. One as you mentioned is using fluorescent nucleotides (all dCTPs are labeled). Another option is: using fluorescent forward primers. This paper might help in case you are super interested in the labeling steps: www.sciencedirect.com/science/article/abs/pii/S0167701205001806

@henrikslab 3 жыл бұрын

Another possibility: Using 3´end labeling with fluorescent nucleotides + terminal transferase (www.future-science.com/doi/10.2144/000112182)

@binebum1 2 жыл бұрын

Super interesting and clear. Wish you could do some videos on the energy metabolism of cancer cells!

@henrikslab 2 жыл бұрын

@@binebum1 I did one about the warburg effect.. check that out!

@prachitrivedi5538 Жыл бұрын

good explanation

@anjalipatel4107 6 ай бұрын

Very nice explanation sir😊

@mohammedal-hammadi5085 3 жыл бұрын

Thank you

@sebastianomoscolin267 8 ай бұрын

🔥🔥🔥

@panosfragalas3161 Жыл бұрын

Thank you alot

@doaaismail11 2 жыл бұрын

this is very clear than the dr. explanation

@user-ef3uq9qe9f 7 ай бұрын

Nice and easy

@ahmetziyaaktas9179 11 ай бұрын

How the cDNAs hybridize with the probes. Probes are one-stranded okay, but aren’t the cDNAs are double-stranded?

@caasinom8008 2 жыл бұрын

How does hybridization occur? Are the oligonucleotide probes single-strands as is the cDNA or is there a thermocycle similar to PCR?

@henrikslab 2 жыл бұрын

Yes, the oligos are single stranded!

@a_9773 Жыл бұрын

Kuss geht raus, brudi

@maryjakr8538 5 ай бұрын

Why would the sample 2 material not binding to the array cDNA mean that the gene is not expressed? Could it not just mean, that there is a mutation (e.g. microduplication) that prohibits the probe from binding while the gene can still be translated and expressed into a protein by the cell in a „loss/gain/change of function“-mutation?

@lstrawaya6403 2 жыл бұрын

Merci

@Unreal2424 2 жыл бұрын

Hi, I got few questions 1 - we agree that we label AFTER retrotranscription during samble preparation? 2 - The oligonucleotides found in each microarray, are complementary to cDNA ? so they are DNA and not RNA ?

@henrikslab 2 жыл бұрын

Hi, 1 - there might be other assays, but mostly you label with fluorescent nucleotides DURING cDNA synthesis 2 - Yes, they are DNA

@Unreal2424 2 жыл бұрын

@@henrikslab thank you !

@sketchy1113 2 жыл бұрын

Very easy😁

@anmolpardeshi3138 Жыл бұрын

the health and disease sample complimentary strands that is put in one tube is from the same person or one is from the case and other is from the control?

@henrikslab Жыл бұрын

Technically, that depends on the research question you are asking, both would be possible

@effiek3886 Жыл бұрын

Can we have more samples at the same time;Does this technique only work for cancerous cells or any type of unhealthy cells;

@henrikslab Жыл бұрын

Yes, you can apply multiple samples to a microarray at the same time. This is commonly done using a technique called "multiplexing". Multiplexing allows multiple samples to be processed simultaneously on a single microarray. It is done by hybridizing different labeled samples to the same microarray, or by using different probes for each sample on the microarray.

@sandrawatt Жыл бұрын

Clear and concise explanation! Question: does each sample go on its own microarray, or are they mixed and applied on the same microarray? Thanks!

@henrikslab Жыл бұрын

Each sample is typically applied to its own microarray.

@henrikslab Жыл бұрын

However, there is also the possibility to apply multiple samples to a single microarray. This is commonly done with a technique called "multiplexing". It is done by hybridizing different labeled samples to the same microarray, or by using different probes for each sample on the microarray.

@sandrawatt Жыл бұрын

Ah, that's why it was confusing. It seemed like "sometimes". This really clarifies it. Hope it helps others too!

@sara-qz8zw 9 ай бұрын

Is there anyone here to help me? . My master asked us to find (the mRNA -DNA ybridization use for which microbes?) Pleaseeee help me

@sara-qz8zw 9 ай бұрын

Hybridization

@HenryGhost-dx9gb Жыл бұрын

how do u make ur animation

@henrikslab Жыл бұрын

To 95% I create everything in Powerpoint, only rarely I also use Inkscape

@bts_rollercosterlifepurplearmy 8 ай бұрын

U save my 7 marks 😀

@urfujji937 6 ай бұрын

Thanks for concising it rather than many slides lol.

@user-xe4yu2om8j Жыл бұрын

Is the oligonucleotide RNA or DNA?

@user-sz9ji9fo1e 2 ай бұрын

Rna only

@muhadsaghaffar746 Жыл бұрын

👍

@evilrubin2455 2 жыл бұрын

Ginge das auch auf Deutsch? :D Nicht sonderlich schwer rauszuhören. Mein ich nit böse, danke für's Video!

@oe4022 2 жыл бұрын

Thanks for this video ! just a small point here 0:45 "tissue from a healthy cell" doesn't make any sense, you should have said cell from healthy tissue or something like that.

@robin2385 2 жыл бұрын

let me guess, you are german, but nice vid