Gram positive and gram negative bacteria (Gram Staining procedure explained)

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Gram positive and gram negative bacteria (Gram Staining procedure explained)

Рет қаралды 134,267

Күн бұрын

Hey Friends,
with gram staining, we can distinguish between gram-positive and gram-negative bacteria. The gram staining assay is based on specific chemicals (Crystal violet, Gram´s Iodine solution, alcohol/acetone and safranine/fuchsine).
References:
serc.carleton....
microbiologyin...
Cheers
Henrik
Time Codes:
0:00 Introduction
0:20 Difference between gram-(+) and gram-(-) bacteria
1:05 Gram Staining
2:26 Antibiotics

Пікірлер: 43

@mariecurie4683 2 жыл бұрын

Henrik, your content always makes my day. Your videos are always so professional, interesting and informative. The amazing graphics make science so entertaining... Thank you very much, keep them coming 🔬

@RookieGamerz-3110 5 ай бұрын

This video is a blessing. The animation and diagram are so perfect that its hard to forget the concept.

@soumyadipsamanta6391 10 ай бұрын

Thanks for this detailed information........ I've seen many videos on KZfaq related to this topic........but it's nearly impossible to find someone, giving the proper mechanism like you did.........majority described that gram positive bacteria cannot take the counterstain, they don't even explain the reason for the decolourisation. But you cleared a lots of confusion.........so thanks again 😊

@nicnac9147 10 ай бұрын

Ikr, English aqa explains this so shit

@SatyaprakashHerode Ай бұрын

Thank you so much , you told all things very easily and in less time and cleared my queries ❤

@sabakanwal9092 Жыл бұрын

By treatment with alcohol the outer membrane of gram negative removed and the crystal violet+iodine bond washed out. As gram positive have thick peptidoglycan layer so this bond of (crystal violet+iodine) is not washed out.. So retained. after that when these both are treated with safranin ( counterstain have almost pink color) the gram negative adopt pink colour and gram positive remain purple due to thick peptidoglycan layer. It is differential staining procedure

@ghousiatabassum3621 Жыл бұрын

Brilliant explanation ✨🌸

@adagross2488 8 ай бұрын

Great easy breasy beautiful microbiology!!!!!

@Auraoll Жыл бұрын

Amazing video thank u so much you explained in to simple yet so effective ‼️‼️

@AjitKumar-du6zf Жыл бұрын

Thanks! For your well animated explanation thanks a lot always sir!!!!🙏🏻

@preciousogie9852 3 жыл бұрын

Your videos are great!

@henrikslab 3 жыл бұрын

Thanks!

@user-tj2pk4nq3s 10 ай бұрын

Thank you for your efforts to peoples to understand it

@nicnac9147 10 ай бұрын

This helped so fucking muchhh, finally something my textbook wouldn’t explain with the staining

@aichabee3014 10 ай бұрын

This video was a really great illustration of the concept!

@peacely191 Жыл бұрын

Awesome video, thank you 🙏

@umc9959 9 күн бұрын

Does'nt the accumulation of pigment occur in the peptidoglycan layer and not in the membrane itself?

@ruggerogabbrielli6831 5 ай бұрын

What does crystal violet bind to exactly when used for the first time?

@btsupdateschannel6865 6 ай бұрын

Thank you so much I understood 🙏

@Burhadedurvesh 3 ай бұрын

fabulous

@Dana-gv7og 2 жыл бұрын

THANK YOU !

@user-kj7zk8lj5k 3 ай бұрын

STL1 are here !

@dina3630 3 ай бұрын

Thank you❤

@user-rk2fm3bn3u 9 ай бұрын

Thank you ❤

@KhushiVerma-de7it 3 ай бұрын

Ooh my dear thaknu thku so much ❤❤❤

@enjoyyoureverymoment4381 3 ай бұрын

Thank you

@ZingClasses Жыл бұрын

Thanks ssir

@slaymarie_rdh_2B 6 ай бұрын

Thanks

@HUMAIRAKHAN_1999 7 ай бұрын

Nice explanation ❤

@melodiousmag 11 ай бұрын

Thank you sm!

@radhickasharma7556 10 ай бұрын

Thankyou ❤️

@arabdrops7009 8 ай бұрын

Amazing video!

@SomeOne-xr7xu 5 ай бұрын

I don’t get why the outer membrane of gram negative is removed ? And where is the role of lipid in all of this?

@gavinwightman4038 5 ай бұрын

The outer membrane is not removed; the crystal violet/iodine is washed out with alcohol in gram negatives due to their much thinner peptidoglycan wall. Gram + retain the violet color.

@gavinwightman4038 5 ай бұрын

The Lipopolysaccharide (containing lipid A endotoxin against immune systems) is on the outer leaflet of the gram negative outer membrane.

@ahmaadkhaan1694 3 ай бұрын

Crystal Violet is washed out in Gram positive bacteria not in Gram Negative bacteria ..?@@gavinwightman4038