Nice tutorial i like the recipe, just a few comments on your other processes. Why did you inject an entire 10cc spore syringe its a waste of spores you could have added a few drops, and did you qc the syringe on agar before to make sure its not contaminated? Also at the end you ruin the sterility of your LC when you add it to the syringe in a few ways. Firstly, assuming you autoclaved the LC syringe, you negated that by placing it on non sterile surfaces, then spraying it with a sanitizer(spraying iso on a sterile object makes it sanitary and no longer sterile, 2 very different things). Second when you pull the sanitizer into the syringe you make the inside of the syringe no longer sterile. Lastly when you run the needle through the micropore tape you expose a large hole to open air then put your fingers all over it. Not trying to hate or anything a lot of people dont have the air quality to work like this without contaminations is all. Here's what I'd do differently: prep: Autoclave the LC syringe in tin foil and keep it wrapped until ready. Use a RTV injection port (you can stab those thick blunt tip LC needles thru it many times) process: Operating in a flow hood or still air box. Prepare by exposing the connection end of the syringe and place it down not touching any surfaces. Open the syringe and slide it half way out of the package, remove cap inside sterile package. Remove syringe completely and attach it to sterile needle by holding the needle from the tin foil making sure never to touch it. You can flame sterilize the needle but its not necessary if you pressure cooked it well. Wipe and spray 70% iso on RTV port. Put syringe in RTV injection port and suck up ur LC. When you finish pull the needle out(the jar remains sterile). Remove needle, you can touch it now. Put syringe in sterile package and secure the cap inside of it without ever touching it.

Psychedelics are just an amazing discovery. It's quite fascinating how effective they are for depression and stress..saved my life.

Psilocybin mushrooms are just filled with so many health benefits. Psilocybin mushrooms treatment saved my life from depression and excessive alcohol use. Never thought I would be saying this about mushrooms.

Thank you Jared for the All in one grow kit. Blessings to you and your family❤

Your channel is the best on KZfaq. We love you!

3:00 Candymaker has a tip for you: Sugar starts to crystalize if you only use one type of sugar with water and heat - no matter which sugar you use (glucose, sucrose, fructose). Corn syrup contains only glucose sugar (usually something like 30% of weight is glucose while the other is water). If you mix glucose with sucrose (in your case something like 1-2g sucrose into the 12g syrup as around 3-4g of the syrup is glucose), it will not crystalize and you have not to worry about it.

I got a 10cc syringe from a reputable dealer. I have used a grand total of 4cc from the syringe and in return I have 10 flourishing, clean agar plates and 3 quarts of popcorn inoculated. The remaining 6cc in the syringe is so dense with spores it's amazing. The agar plates are for isolating strong specimens, then the LC comes from my favorite from that. For a $21 syringe, you can do a WHOLE LOT. For sheer ghetto points, I have never even screwed the provided syringe on. I have used 4cc of spores completely bareback from the lurelock port. I observe reasonable aseptic techniques but do not have a laminar flow system. None of the 10 agar plates are contaminated.

Brother, your videos are fantastic, informative and straight to the point! Thank you for all you do! ❤

As I am just starting out in this endeavor your video simplifies what I had always imagined could and is being done- thank you!

Easiest, most straightforward video ive seen yet.🙏🏻

Give yourself another gift: If you tab the tape on the roll when you're done, it makes it easier to pull for next time, plus you already have your tab ready to go! Great videos and love your calm energy, thank you!

This is the only video I've watched like this! Thank you!!

Nice work! Getting ready to do some of these myself and the information is very appreciated.

Thanks for sharing this concise and informative coni👍🏼

So helpful for a noob like me. You’re the man!

Thank u so much this looks so much simpler than I had anticipated

You seem pretty chill. Going to check out more of your videos.

Very interesting. I'm just starting to learn all this stuff and that was very helpful. Thank you.

Thank you, using your website. Enjoyed the video, like your style.

Thanks for the rad work!

Awesome video! Thank you for the lesson!

You made it look so easy!!

You crack me the hell up, love you broski.❤

What a great pedagogue you are. Can't stop watching your videos. Little question here. Where (i guess fridge) and how long does your liquid culture keep?

Thanks. I had given up on trying to cultivate at home until watching this.

Really great hair day for sure bro. That's really all that matters in life

Cool video bro, live culture rocks!

Thank you for reporting in grams and millimeters. :)

Thank you so much, great video

Hey brother Love your channel Thanx for all the information you provide Also busy with my own cannabis and mushies channel Mad love bro Peace

Great video 🤘

Very informative video, very helpful. 🙏🏾🍄

Very helpful! Thanks!

Hey brother Hope you are well Just wanted to tell you that your channel inspired me a lot I'm also trying to start my own channel now growing cannabis and mushies Mad luv Peace Mad scientist

You are a rare breed on KZfaq, sir. Your videos are direct, not gimmicky, easy to follow, and delivered in a professional manner. Thank you for your efforts. One question. What gauge is the blunt 4 inch needle?

+1 subscriber my man! good work

bro thats easy af, thank you!

Thank you friend! This is important information!!!!!!💚 "Thank you for watching." Thank you for making!!!!!!!!!!!!!lol

@4:35 - that is the most beautiful Snow Globe ever :)

Great channel.

Thank you so much

Ya man, you look fabulous! grin.

Awesome vid

Thank you 🙏🏻

LME liquid malt extract will show growth in a couple of days, i used to use karo but LME is a game changer!

Thank you

I loved the proprietary joke 😂😂😂

It takes 1 to 3 weeks for lc to colonize. Thanks for the updated steps!

Thank you for sharing. off to have a mooch on your site..

Rock on buddy thank you

Thank you!

Just wanted to add on the contamination worries especially for us first rate newbies,.. if its too overwhelming to make mason jar blenders and opening jars etc,.. make the liquid substrate jar with an injection port,.. and inject bought liquid syringes. This is not to knock the poster’s skills,.. i just find this video very advanced compared to where i am at this point in time. Also i live in a 200 year old house from hell (building a new place thankfully) but theres every possible way to contaminate in this house i am in right now. It would increase my chances of success if i bought a pre done spring and injected it into a sterile jar of liquid culture. I can learn to make that jar, to add nutrient and inject sterile air etc. but making the Petri dish, cutting it etc thats a ton of opportunity to add contaminants when done by me😊.

Wow.. thankyou.

Thanks

What are the "nutrients" u inject in jar prior to pressure cooker process?

Thankyou

Just found you, I subbed!

Hi. I just found you and have enjoyed the videos I’ve seen so far. I’ve also checked out your website. I’m very new but already looking ahead. I was wondering was size your pressure cooker is. I will certainly be a customer on your website.

Hahaha 😂 dude is WRECK’T! I love it

do instant pots work in place of a standard pressure cooker?

So if I already have a 10cc syringe of LC, how would I multiply that. Would I follow the same steps, just instead of 10cc of MS use 10cc of LC?

Can you transfer a grow bag with mycelium to a monobox with substrate? Ty

Thanks for this awesome video, I gotta question, can I use Honey instead of kernal syrup ? If not, what else can I use in case I dont find kernel syrup in my country please

thank you

Just ordered my starter kit. Been looking for something like this.

Thanks for the videos im on 15days n there is still alot of spores in my jars did i do something wrong infollowed everything to the t

Awesome video dude! Question, I want to do this but syringe is a live culture syringe with mycelium in it instead of spores. Can I do the same process and just inject the jar with the mycelium I already have instead of spores?

Great video. Thank you for your information. So now you have 10 30cc syringe where you drew mycelium (not spores in through a 12ga needle. When you are ready to dispense into spawn jars or bag,, do you have to use same sized 12ga needle? Or a more standard sized injection needle?

Hey man! Is the rock special or is it just for mixing the LC around instead of using a magnetic mixer?

Do you admin 10cc liq cult into 5 lb all-in-one. The same as you would 10cc spore?

THIS IS ONE THE BEST CHANNEL I HAVE COME IN CONTACT , PLEASE TELL US THE NUTRIENT YOUR USING . IS IT LME PDA SYRUP HONEY PEPTONE??? REPLY BEFORE I MAKE MISTAKE THANKS AND REAMEN BLESSED

Can i use lc instead of spores? Just putting 10cc Liquid culture in the jar?

Nice hair. So do you see contamination in the LC or do you just roll the dice? Is it safe to assume that the same thing can be done from LC to LC? How manc CC of LC would you add to 800ml of solution?

I would like to see you test with using biochar in the substrate. You would probably have to innoculate it with the rest of substrate. Still think it would be beneficial, especially on multiple yields.😁

Can you use jelly powder(no gelatin) instead of agar powder in Petridish? Pls reply fast

Would you knock a jar up with the whole 10cc of spores, or how much per jar? Plan to use 600ml LC.

In order to get mycelium growing the quickest… is it faster to turn spore syringes into LC rather than just inoculating my grain with the spore syringe?

What kinda nutrients do you use in that first syringe?

great videa dude

If I want to use peptone and malt extract how much of each do i use?

Did you use a cabinet against air germs?

Really great vids. I like your approach.

New to this can mycelium look like powder in the grow bag I'm having trouble growing mycelium

Will the alcohol kill/ruin the spores if it doesn’t dry completely? I noticed when you sprayed the top before drawing the spores up it was very wet still and again when you sprayed the stopper you put on until you use a needle, do you wait for the alcohol to dry normally?

I had an issue or problem that maybe someone has suggestions for. I had two setups, identical to yours in a pressure cooker, and water boiling at 20 psi (vs 15) caused rock to jump around tapping the bottom of the jar and splitting the bottom off both 312mg setups. Just like tapping glass for a controlled break the bottom was 360 tapped and broke. Any suggestions other than not have a rock in there? Great sterile technique. My water level was a little deeper than yours and just touched the glass bottom

I have about 4 mls left in a syringe I purchased tobtry making grain spawn cud I try this with what's left?

Just wondering if the pressure cooker is absolutely necessary here? Can I just use my normal Tefal pot?

Can you keep repeating this process with the culture you make? meaning starting the process over again but with the lc syringes we just made?

If I only have 1cc of liquid culture in a syringe will that be enough to start a new jar of liquid culture?

Your hair really does look excellent today 😂

Did u make the lions mane harvest video from two years ago?

Does anyone know a substitute for corn syrup? For some reason I can't find any in my area. Also how long to keep the jar before extraction of the mycelium after all the process shown in the video?

Would Senescence occur the same way as grain 2 grain does?

If u add honey or kyro syrup and place a heating pad near it u could have tripled that growth and if u used that spore syringe and did 3 different jar tripled the amount as well. Still a great video tho!

Great job! Good info and nice hair day:)

do i immedietly inject or do I have to store it for like a week, or two?

GOOD LUCK TO YOUR WORK SIR, AM ABOUT TO START NOW , THE NUTRIENT YOUR USING IS IT LMA OR AGGA AGA. NUTRIENT AGGA ? PLEASE REPLY BEFORE I MIX . THANKS AND REAMEN BLESSED

Great video I spend like 50.00 at a time on syringes....do you use distilled or bottled water for fruiting and mycelium instead of tap

If I want to grow mushrooms indefinitely, but will have periods where I won't always be able to fruit, is it better to keep mycelium going through liquid cultures or grain to grain?

The alcohol not being dry before injection doesn’t kill the spores? How?