There are so many tools and software available to calculate compensation! Which options are the best to use? The goal of this video is to help you decide which route is best for you. The second part of this video will provide some further tips with FlowJo.
0:00 Intro
1:06 Decide which route - Question 1
1:30 Decide which route - Question 2
3:29 Decide which route - Question 3
4:36 Decide which route - Final comments
6:35 FlowJo Tips - Is there a compensation matrix inside of the FCS file?
7:39 FlowJo Tips - Change the acquisition defined matrix
8:34 FlowJo Tips - Apply the matrix to samples
9:10 FlowJo Tips - Create a new matrix
10:16 FlowJo Tips - Saving and exporting options
Resources:
General compensation resource: voices.uchicago.edu/ucflow/pr...
How to properly set up gates in automated compensation tools: voices.uchicago.edu/ucflow/20...
Tips for identifying bad data (including compensation errors): voices.uchicago.edu/ucflow/ca...
Tips for fixing compensation errors:
voices.uchicago.edu/ucflow/20...
Learn about compensation in FCS Express on the DeNovo KZfaq Channel: • Compensation (Full video)
Learn about compensation in FlowJo on the FlowJo KZfaq Channel:
• Traditional Compensati...