How to Focus Using Oil Immersion Microscopy (1000X)

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How to Focus Using Oil Immersion Microscopy (1000X)

Рет қаралды 66,887

Dr. Gary Kaiser

Күн бұрын

Пікірлер: 25

@chrrmin1979 6 жыл бұрын

How many people aren't here for school? Just a love for microscopes

@amakaa.541 5 жыл бұрын

This was last minute video before my exams. Thanks alot🙏🏾 it was helpful

@Sagar-lp9ek 2 ай бұрын

This video is Gold.

@jadenbroadway173 4 жыл бұрын

Thank you, I am working on mastering this task. I have class in the morning, can’t wait to try this.

@newworldorderordis-order6803 4 жыл бұрын

thank you dr. next week I got midterm for my mlt program. something u taught me in case my microscope gives me drama while doing my practical slides

@zinnyeera8605 3 жыл бұрын

Thank You so much!! I really Needed this!🤝

@pequodexpress 5 жыл бұрын

Why not first focus with the 40x, move to the intermediary position between the 40x and the 100x, place a drop of oil on the slide, and then move to the 100x?

@StoryoftheEye 5 жыл бұрын

Edward Santoro, that is how I do it, hah.

@lajoswinkler 7 жыл бұрын

This is a very dangerous method that can ruin both the objective and the specimen. Long before 100x objective is used, coarse knob is not turned at all. Coarse adjustment knob is used with 4x and 10x objectives, then a 40x objective is set and image is focused with a fine adjustment knob. Revolver is set between 40x and 100x, immersion liquid is added on the slide and 100x objective is lowered into the drop and rocked a bit to avoid air bubbles and ensure complete wetting. Then the fine focus adjusting is a matter of tiny turn because 40x and 100x under oil have nearly the same focuses. 100x is so close to the specimen (around 100 micrometres) that moving the coarse adjustment is a good way to press the objective into the slide which often results in damage. Your method requires lots of turning of the fine knob which is detrimental to the fine adjusting system. It loses rigidity that way. I've seen it lots of times with students.

@rhwinner Жыл бұрын

Is there a danger of the 100X objective colliding with the slide when going from 40X to 100X objective?

@teddlouise.coloma906 9 ай бұрын

@@rhwinnervery much so

@varunthingom7270 8 ай бұрын

@@rhwinnerabsolutely not if you have focussed in 40x

@ahix3348 11 ай бұрын

Great video! Thanks.

@khentanthonyabella9068 7 жыл бұрын

Hi Dr. Gary! I have difficulty viewing the allium cepa root tips. I cant see clearly the cells of the root. Can you you please give me some techniques to view it clearly .Hoping for faster reply thank you

@suzettetorres7064 Жыл бұрын

What happens if you using oil immersion on 100x and accidentally go toward the labeling ?

@knightingalesaid 7 ай бұрын

Thanks!

@mihaellazar9645 7 жыл бұрын

NIce video very helpful.

@garyekaiser 7 жыл бұрын

Glad you found it useful!

@mihaellazar9645 7 жыл бұрын

Finnaly got my microscope.But i was looking at a blood puddle and my microscope couldnt focus on the puddle but kept going out of focus.Im pretty sure this is beacuse i have old plastic top cover for the slide and its not a problem on my microscope but if it is i need help.

@saturatedk9674 7 жыл бұрын

Hi! i'd like to ask, isn't it necessary to use scanner, low, high then oil immersion objective? or is it alright to just skip all 3 then to oil immersion?

@garyekaiser 7 жыл бұрын

When looking at objects that can be seen when using a 10X objective (100X) starting at low power and rotating the objective lenses to obtain higher magnification works well. When viewing bacteria, most are too small to be seen when using a 10 X objective. In this case it is faster to start right out with the 100X oil immersion objective (1000X) as described in my video.