Our novel REAlease® MicroBead Technology allows for magnetic cell isolation with reversible cell labeling. By the removal of any beads or labels from positively isolated cells, you can be more flexible with any downstream application you can think of. Isolate cell subset of interest by sequential isolation, or just get your cells free from any labels. Explore more www.miltenyibiotec.com/produc...
Untouched isolation of cells from challenging samples, such as tissue or PBMC suspensions with abnormal cell frequency distribution, is hard to achieve. REAlease MicroBead Technology allows sequential isolation of cells to get to your cell subset of interest. REAlease Technology relies on engineered antibody fragments that, when in a monomeric state, have a low affinity for an epitope. When multimerized in a biotinylated REAlease Complex, this complex binds to cell epitopes with high avidity. Together with a REAlease MicroBead, the target cell is magnetically labeled. During magnetic isolation, labeled target cells are retained within the column and thereby separated from non-labeled cells. To get MicroBead-free target cells, the REAlease Release Reagent is applied to the column during the elution step. The eluted target cells are free from REAlease MicroBeads and can be subsequently used for a second round of magnetic isolation e.g. using regular MACS MicroBeads. Label-free target cells can be obtained by addition of the REAlease Release Reagent. This leads to monomerization of the antibody fragments and dissociation of the complex from the cell surface. The result: cells that are free of any labels. As their epitopes are completely available for re-labeling, the cells are suited for any downstream application.
Miltenyi Biotec is a global provider of products and services that advance biomedical research and cellular therapy. Our innovative tools support research at every level, from basic research to translational research and clinical application. This integrated portfolio enables scientists and clinicians to obtain, analyze, and utilize cells. Our technologies cover techniques of sample preparation, cell isolation, cell sorting, flow cytometry, cell culture, molecular analysis, and preclinical imaging. Our more than 25 years of expertise spans research areas including immunology, stem cell biology, neuroscience, and cancer, and clinical research areas like hematology, graft engineering, and apheresis. In our commitment to the scientific community, we also offer comprehensive scientific support, consultation, and expert training. Today, Miltenyi Biotec has more than 2,500 employees in 25 countries - all dedicated to helping researchers and clinicians around the world make a greater impact on science and health.