There are maaany things missing from this but from the top of my head... - Wear suitable gloves to avoid touching the column with your bare hands. - When you cut the column with a wafer, you need to make a quick score with the smooth edge of the cutting wafer and essentially flick the end to reveal a clean cut. You should never need to bend the column or snap it. Always check the cut with a magnifier (jewellers loupe works well) to ensure the cut is clean. A poor cut will affect the chromatography. Wipe the end with a lint free cloth and solvent (methanol works) and always cut after inserting the ferrule to avoid a blockage. - With a new column, you need to purge at a low temp with a suitable carrier gas prior to conditioning to remove oxygen. This will vary based on the diameter of the column (narrower = longer purge time) You will also need to leak check your inlets prior to conditioning the column. Heating the column to a high temp with oxygen present will seriously reduce the column life or even destroy it before first use. These are some really basic principles of column insertion.

There are several things missing from this installation video, you should cut the column after installation of the ferrule, it is best practice to slide the ferrule on with the column end pointed down to prevent tiny ferrule fragments from traveling down the column past where you'll cut it. Then you must inspect your cut, it must be square - if not, cut it again. When cutting you just want to score the outer surface, then gently apply pressure to bend the capillary, it should break cleanly and with an even edge with very little pressure and bend angle, if you can still bend it, you have not scored it enough. If you choose to use no gloves it is best practice to always wipe the tip of the column with MeOH to remove contaminants, while you mention fingerprint oils, the truth is your hands are also covered with fragrances from hand soaps, oils from foods, and numerous other organics which yes may be baked off, but they will be baked off in your inlet and detector and cause you baseline headaches- best to just clean the end with MeOH. - This is especially true when using mass spectrometry instead of a simple FID detector. Your installation procedure also shows you using knurled finger tight nuts which are not standard and are not the ones you demonstrated on the bench. Those standard nuts should be fingertight then an additional 1/4 turn by a wrench to compress the ferrule, especially when using 85/15 ferrules and not 100% graphite. Finally, installation is not complete until you condition the column - it is my personal preference to always condition the column with only the inlet attached because I want to be as clean as possible. You must first purge the column/inlet of any air for about 10-15mins, then you can start heating the column at a nominal flow rate and ramping up to your conditioning temp and holding for several hours. - Then you can install the detector side and proceed with a Grob test injection to check proper installation and column activity.

I would add that the column should get cut after the ferrule has been inserted onto the column, it is common for graphite to block the column. Very helpful video !

Very very thank you for update this kind of concept.

Thank you for teaching🥰india

It's nice to see the ancient 5890 series X)

A cordial greeting, where I can obtain information on the frequency with which consumables or parts should be replaced either from the injection port, column or detector

Thé cutting of the column must be done just before connecting the edges to injector and detector. In addition, it has to be wiped with soft cloth or a piece of cotton and methanol.

Will the Septa remain after fixing the Nut or removed?

Thank you

Tnx madam🙏🏻

Thak u

This was SO HELPFUL thank you!!!!

this video is SO wrong you're touching everything with your bare fingers leaving your bodily oils on the column and ferule, and plus cutting before you install nut and ferule... very bad practice, good luck with your baselines.

Uploaded the usefull videos

good video, good girl.