How to Stain an SDS-PAGE gel

Рет қаралды 168,816

13 жыл бұрын

Your gel just finished running -- what do you do next?
In this video, Dan shows you how to disassemble your SDS-PAGE gel, and how to stain it using either PageBlue, Coomassie, or Silver Staining.
Coomassie is the older method of staining, and gives bright blue bands on the gel. PageBlue is similar, but provides a quicker alternative. If you have faint bands and require a more sensitive method, you can use Silver Staining.
Choose whatever method you need, but beware of the hazardous materials that may be used.
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Пікірлер: 40

@Findulidas 11 жыл бұрын

Very useful. My teacher/lab supervisor had us set up a whole sds-page lab from scratch complete with method, volymes and what we do how in what order. We had to find information ourselves on how to do it using books and online. Most of us had not seen this been done before. These clips really helped me to visualize what Im supposed to do which is something modern teaching has forgot the imporance of.

@89WinterButterfly 11 жыл бұрын

Thanks for posting these videos. You've been very helpful!

@carlypenaemprende 9 жыл бұрын

So useful! But i would like to see the final result comparing the three methods.

@ajunahenry9307 3 жыл бұрын

brief to the point. smart teacher

@dariacirlan8538 4 жыл бұрын

was looking at this out of curiosity for my MBB222 class at SFU when I realized where this was filmed haha. Great video though!!!

@DenisonLove 8 жыл бұрын

Great video! What should have been said for silver staining is that band intensities CANNOT be directly compared in silver staining. I forgot the precise reasoning, but certain amino acid rich proteins are more likely to bind with the stain, hence, bands cannot be compared.

@labtricks 12 жыл бұрын

@osmarguz you can buy it directly from the manufacturer, Fermentas, which is now a part of Thermo Fisher. Try looking at their online catalogue for the prices

@beattypinkfairy 3 жыл бұрын

Thank you so much!!!

@panoskre Жыл бұрын

Thank you!

@rhodnius 3 жыл бұрын

Hi, thanks for sharing. I have a question. What's the reason for microwaving the gel? Thanks :)

@kylehasgeniusbits 7 жыл бұрын

I have a question, what about the next step of preserving your gel in drying solution? What's the best way to do that?

@IWantTo0wnMyLife 10 жыл бұрын

Reviewing for job interviews. Thanks a lot, these were really helpful videos.

@1091Floyd21 8 жыл бұрын

+IWantTo0wnMyLife I can't imagine HR asking about this!?

@federicoruiz1749 3 жыл бұрын

@@1091Floyd21 Not HR, but the technical interviews can ask these kind of things... jajaja nevertheless, the position has to be very specific for this type of technique to be employed since there tend to be technical people that excel in this, right?

@TheDKDEO 2 жыл бұрын

can you able to provide detail protocol how i can run a page gel and try to do stain. please help me to learn

@TheNawoola 5 жыл бұрын

thank you

@denebss 11 жыл бұрын

Hairstyle 5/5!!

@komals.6881 5 жыл бұрын

Hii make a vedio on native gel electrophoresis too

@laurenmabe4874 6 жыл бұрын

OK, so I tried to stain my membranes and got really poor results. My standard bands are clearly visible and did absorb the Coomassie blue, but no sample bands were stained. Was this because I stained the membrane as opposed to the gel itself, or will it also work on the membrane? Thanks!

@coolkolu 12 жыл бұрын

we generally use Coomasie Blue fr staining..

@amandac8483 8 жыл бұрын

great!

@namra7256 3 жыл бұрын

Please show the end results too !

@waningpoetic 3 жыл бұрын

I miss y’all

@CherryMoch 13 жыл бұрын

can you make one for western blotting? thanks! :)

@dr.husseint.altamimi6589 10 жыл бұрын

very useful video>> Like.

@osmarguz 12 жыл бұрын

where I can buy the pageblue?

@nidhivijayan4451 2 жыл бұрын

What does the gel look like in the end??

@DanzQueen 12 жыл бұрын

Oooh yeah, Page Blue is new. Never used it. Must try...

@andreialourenco796 8 жыл бұрын

Yes. This video is great. But i would like to know what is making my buffer get too much hot while my SDS-Page gel is running. And if it can damage the proteins. Thank you.

@nguyendung0206 8 жыл бұрын

the electrodes. U need cooling system since heat can damage the gel

@andreialourenco796 8 жыл бұрын

+Nhím니임 oh. OK. thank you.

@teresastories11 3 жыл бұрын

I’m the guy on the door who is softly drinking his coffee

@p.m.satyanarayana8505 2 жыл бұрын

I saw my gel getting shrunk after staining & destaining (CBB). Staining- 10 mins- generally I stain it for 10 mins & destaining-30 mins.

@p.m.satyanarayana8505 2 жыл бұрын

I have been staining & destaining for 10 mins and add water & leave it overnight. Bands always were visible clearly.

@labtricks 11 жыл бұрын

Distilled water should be fine to separate the gel, and then follow your procedure as usual for Western blot. Just remember to use clean gloves when handling this gel, the membrane, and filter papers during the process.