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HPLC TIPS FOR ROUTINE PROBLEMS | VOICE OF KAYANI
Рет қаралды 826
Күн бұрынHPLC TIPS FOR ROUTINE PROBLEMS | VOICE OF KAYANI
This video based on HPLC tips for routine problems.
in this video explained 10 important point and tips for HPLC problem and solution.
thanks for watching.
your quires;
HPLC
troubleshooting
HPLC problem
HPLC routine problems
HPLC routine troubleshooting
trouble
shooting
HPLC interview questions
#voiceofkayani
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#hplc #tips #troubleshooting #interviewquestions
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@vasudevsharma-ey6ni Ай бұрын
Hplc vials ko wash karne ka best tarkika
@voiceofkayani5419 Ай бұрын
Keep dipping in solvent for washing your company using ..which is validated cleaning procedure...and use methanol also for washing and dry in over..... Will make a video again on it
@AjayThakur-pj3db Ай бұрын
Sir standard or test solution kaise calculate kare is pr video banaye
@voiceofkayani5419 Ай бұрын
Ok done
@vasudevsharma-ey6ni Ай бұрын
Sir hplc ki 2nd means organic solvent washing ke case me solvent kis base par decide hota hai ? .....kya sabhi cases me methanol use karte ? Hai ?
@voiceofkayani5419 Ай бұрын
Agar mobile phase Mai methanol use huwa hai to organic phase methanol ka hoga....and agar acetonitrile use huwa hai to organic phase actronitrile use hoga... Normally depends k Kiya solvent use huwa hai HPLC analysis Mai
@vasudevsharma-ey6ni Ай бұрын
@@voiceofkayani5419 but sir is it true that acetone or acetonitrile is not good for hplc ...it may degrade column ?
@vasudevsharma-ey6ni Ай бұрын
Hume hplc column Kab aur kaise discard karna chahye ...
@voiceofkayani5419 Ай бұрын
Ok the basis of theoretical plats
@vasudevsharma-ey6ni Ай бұрын
@@voiceofkayani5419 no. Of injection ? Based ?
@vasudevsharma-ey6ni Ай бұрын
Sir ...kya uv calibration ki frequency kya hai ? Kya ye fix hoti hai or company wise hoti hai
@voiceofkayani5419 Ай бұрын
Fix as per best practices.... 6 months calibration frequency
@vasudevsharma-ey6ni Ай бұрын
@@voiceofkayani5419 any guideline or practice based ?
@voiceofkayani5419 Ай бұрын
@vasudevsharma-ey6ni based on best practices of multinational pharmaceutical industry and you can check WHO , ICH GUIDELINES and also ISO 17025
@itzkalaakaar1015 Ай бұрын
Sir GC mai sample inject karne ke lie moisture ki kya limit hai? I mean kitna percent moisture ho to sample inject kar sakte hai?
@voiceofkayani5419 Ай бұрын
It should be under 1‰
@voiceofkayani5419 Ай бұрын
It should be under 1 %
@vasudevsharma-ey6ni Ай бұрын
@@voiceofkayani5419 sir agar moisture jyada ho to kya koi impact hota hai
@voiceofkayani5419 Ай бұрын
@vasudevsharma-ey6ni affects the analysis
@vasudevsharma-ey6ni Ай бұрын
Agar internal diameter increase Kar de to RT par kya Asar hoga
@voiceofkayani5419 Ай бұрын
RT pe asar nai parega. But peak broad ho jaegi
@vasudevsharma-ey6ni Ай бұрын
@@voiceofkayani5419 aur particle size increase kare to kya farak pdega
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