You can see in his eyes all the time he has dedicated in his life to this subject he loves so much.

could not of asked for a better, well presented explanation of fluorescence. Very nicely done.

Thank you so much..no one has explained as good as you. Thank you iBiology🙏

Thank you so much for this presentation, for the very first time I actually get it!

Amazing lecture very simplified but of high level. thank you very much it was very helpful.

Thank you for knowledge. 😊❤

Excellent video, would be nice to see a tutorial on specimens preparation and various staining techniques. I have found specimen preparation and technique is more important than the mechanical understanding of the process. I am sure your techniques would be another excellent tutorial from you! Thanks

love it! At last I understand how those "cubes" really work!

Very well explained, thank you so much!

very well explained,video makes clear the principle and working of Fluorescence microscopy,thanks

Excellent lecture and demonstration. Really impressive.

Great, clear, and informative presentation. Thank you

Such a wonderful, in detail presentation. Thank you Sir

Your way of explanation by using tools is really aid in learning....thank you for making such nice and helpful video !!!!

Is there a microscope which allows to collect all these types of exposures at once for a detailed (colored) video compilation of samples in motion? Rather than via a turret limited to one exposure at a time?

great explanation! thank you!

Great job, well explained, thak you for everything

Perfect presentation, thank you very much

Thank you so much! you explained really well.

These videos are amazing. Thank you

Thank you for this helpful introduction. I believe the quantum efficiency (QE) explained in this video is more commonly referred to as quantum yield. QE is a term used for the camera or detector that is capturing the photons.

It's a very complicated subject, and you are doing a good job here. Thanks

Thank you! Very clear lecture.

I have my first course in nanoengineering, this really helped, thanks a lot!

Great presentation, thank you!

Really, really great! So helpful!

This video help me a lot, I use alpha-tubiline antibody, a lot of time to see, reveal microtubules in the cell, but the image (spindle) was not clear, after watching these videos, I did and it works. Thank you.

thank you so much - really helped me get into my term paper about fluorescence microscopy

Love it, thanks Nico! :)

Good job. This is a very thorough lecture.

im korean, and i'd appreciate this video that is so intutive and well explained

that was great, i like it so much, it helps so much... thank so much for all of this great demonstration and information thaaaaaaaaank u sir

Thank you! I am a researcher who works with polarizing microscopes and I am trying to get into fluorescence work. Out of a dozen video lectures on the topic, several textbook chapters, and a pile of research papers, this video is the first time that most of this has made sense. I especially appreciate the detailed description of the filters, most lectures leave a LOT out on those. I still have some advanced questions about the light source, and how UV fits into this (is it also produced by the Hg lamp?), but I finally feel that I am making progress on this!

Perfect video!!! Thanks so much

Muchas gracias, muy útil, muy amable, muy bien explicado

> Thank You so much for uploading it

Thank you for this video! Very helpful

Well presented, thank you

excellent video!! thank you!

Thanks.. awesome video!

thanks for the video!

Thank you for nice video.

haha play 32:19, he says fkd up instead of fluorescent :P Great lecture btw

thanks for your video

Really awesome explanation Sir

Great video!

frodo teaching me biology, gotta love it

Great video, do you know how to clean the oil inside of the objective? Thanks.

Incredible ! Than you

Thank you for the clear and thorough presentation. Is fluorescence microscopy in general really quantitative, though? The local environment of the fluorophore can have an effect on the fluorescence quantum yield and the fluorescence spectrum of the fluorophore, making it difficult to draw a direct correlation between fluorescence intensity and fluorophore concentration. Am I right? I guess this problem can be circumvented by using fluorescence lifetime imaging microscopy, in which the information (i.e., the fluorescence lifetime at each pixel) is independent on fluorophore concentration. Also, no energy is lost during internal conversion, since internal conversion is an isoenergetic process. The excitation energy is lost during the vibrational relaxation process that takes place after the internal conversion process.

Thank you for the education on fluorescence microscopy. I need to capture a live video of bacteria reaction/dying in presence of house hold disinfectant such as Clorox and Lysol drops. What equipment is best for capturing the video? Would a LED based Dark-field Microscope be good enough or should I consider buying a Fluorescence Microscope and if so what is a good cheaper brand I should look at?

hi there, I would like to know about the protein in jelly fish that you can inject into organelles, how does that work. do you have any video you can upload please? thanks

Excellent!

Amazing!!!

Thank you very much

Thank you

Hello, excellent video, I need help, I would like to know which ones, with the advantages and disadvantages of fluorescence, sorry if you do not understand the question well, I speak Spanish.

this is really helpful. But please help me how i can adjust emission and excitemnt wavelength 490 to 550nm. And how can i adjust the contrast to make the background totally dark. Please help!! I dont know anything about fluorescent microscope and really i am in need!!!!

12:00 I'm a bit confused by the diagram. Is S0,0 and S0,2 the same energy or is S0,2 higher? Because it looks like there's a photon of the same wavelength being emitted when an electron drops from S1 to S0,0 and S0,2 but that can't be if they're different energy levels, right?

i have a question. Actually which electron will go upper state. Here the higher energy state of which molecule? If single electron go up, then sometime spin change, why?

at 16:02, he says that there's an intereference of light. what causes the phase difference for interference to occur ?

good video! p.s did you have a haircut between the scenes? :D

can you explain a negative stoke shift?

great video tutorial for a starter. Explains really well and elaborated. I appreciate. One thing I need to know is about the bandwidth concept. What is the narrowest bandwidth we can use? SO, is it good to use as narrow range as possible?

what is the name of the multiple cube holder?

good one

Does immunofluorescence microscopy emits x-ray? Or gamma rays? How much immunofluorescence microscop safe??

why dying inner cell and extracellular ?

bbbeautiful sir

很棒啊

You cannot really claim FM to be quantitative, because in most cases what you are imaging is the fluorescent labels you have added to your sample.

helpfulvideo

what is differance between Fluorescence imaging and florescence microscopy

You can analyze colocalization in fluorescence microscopy images on iPad using CoLocalizer app, free from App Store: itunes.apple.com/app/colocalizer-for-ipad/id1116017542?l=en&mt

good

that's my uncle :)

👍🏻♥️

Rutger Hauer between acting jobs.

IGNOU BSCBCH 😁😊🚩

Fluorescence die during experiment ;P

unsharp images for much money