Microscopy: Cameras and Digital Image Analysis (Nico Stuurman)

  Рет қаралды 24,233

iBiology Techniques

10 жыл бұрын

Learn more: www.ibiology.org/talks/digital-image-analysis/
This lecture describes how digital cameras for microscopes work, what a "pixel" is, Nyquist sampling, the dynamic range, noise, and color cameras. The features of an image is described, included bit-depth, intensity scaling and histograms, file formats and basic image processing.

Пікірлер: 21
@jeremyschissler337
@jeremyschissler337 11 ай бұрын
Would love to see these updated being almost a decade old
@bilgeargunhan9361
@bilgeargunhan9361 3 жыл бұрын
I've been performing fluorescence microscopy experiments for years and was mostly trained on the job. Having watched this video, a lot of things that didn't make sense before, both in data acquisition and analysis, have clicked. And it only took ~30 minutes. Must-watch for all students/researchers employing fluorescence microscopy experiments!
@seamus9898
@seamus9898 Жыл бұрын
Resolution limit = 2* (pixel_size / Magnification) This is literally all I needed but it was nowhere to be found. Thank you for this!
@Helmet_Tester
@Helmet_Tester 9 жыл бұрын
You are a Great Teacher! I really enjoyed this Lecture and am grateful for all the people who take the time to post these videos. Best Regards, Jerry
@barboramartinkova8452
@barboramartinkova8452 4 жыл бұрын
I totally love your videos, Nico. They are easy to follow and very well-explained. You helped me a lot. Thank you very, very much!
@leif1075
@leif1075 Жыл бұрын
Why are higher bits necessaroly.brighter..that's not true necessarily..they might all be darker and therefore dark..
@roloman1987
@roloman1987 8 жыл бұрын
Thank you so much for doing this. You were incredibly clear and concise. Very very useful lecture.
@genefreak2
@genefreak2 10 жыл бұрын
Thank you! That was a fundamentally useful tutorial. Respect!
@Anilkumar-zz6lo
@Anilkumar-zz6lo 6 жыл бұрын
I am in love with your teachings. Keep going sir. Thanks
@Ishijah1
@Ishijah1 4 жыл бұрын
Excellent explanatory skills.
@ornestebuitkute9720
@ornestebuitkute9720 3 жыл бұрын
This is really good, thank you!
@jaychiang1688
@jaychiang1688 2 жыл бұрын
Wonderful explanation!
@nonameartiiist
@nonameartiiist Жыл бұрын
I love your t-shirt btw!
@Theodorus5
@Theodorus5 6 жыл бұрын
Good job Nico
@tymothylim6550
@tymothylim6550 3 жыл бұрын
Thank you very much from this video! I learnt a lot even though I am not doing biology :)
@jeanpaulpadillafabian1639
@jeanpaulpadillafabian1639 3 жыл бұрын
Great explanation about image analysis ! Where can I get more information about the software development? I would like to apply this for analyzing mineral microscopic images. Best regards, Jean.
@greenbeecolony1911
@greenbeecolony1911 Жыл бұрын
Thank you good video
@ratan483
@ratan483 5 жыл бұрын
sir plz exaplain how the three intensity mix to find original image
@rasoolgh5025
@rasoolgh5025 3 жыл бұрын
Can I use a normal digital camera and analyze the particle using the image I take with it ? what is a proper lens for it ?
@abbasalfaouri9955
@abbasalfaouri9955 3 жыл бұрын
what is photon conversion factor? can you make it in front of us?
@user-zw3gx9rb5v
@user-zw3gx9rb5v 2 жыл бұрын
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