Indirect ELISA

Рет қаралды 249,785

14 жыл бұрын

( www.abnova.com ) - The indirect ELISA is used primarily to determine the strength and/or amount of antibody response in a sample. In the assay, the antigen of interested is immobilized by direct adsorption to the assay plate. Detection of the antigen can then be performed by using a matched set of primary antibody and conjugated secondary antibodies. More videos at Abnova www.abnova.com

Пікірлер: 33

@MonsieurLavabo 12 жыл бұрын

Holding the pipette vertical and pre-rinsing the tip will help you get more accurate results and reduce the variation between wells.

@heysup8889 7 жыл бұрын

this video with that song was much of a life changing journey as it was an educational video, haha thx for the video !

@otienorose7674 6 жыл бұрын

I like the way the video shows in details on how to carry out indirect ELISA test

@ABNOVA1 14 жыл бұрын

We don't have name for background music but we like it as well. Thanks for your support!

@vonguoinoithang 11 жыл бұрын

Thanks for video! It gives me more information about indirect ELISA tech.! Very useful!

@MonsieurLavabo 12 жыл бұрын

@0102murphy No, the worst scenario would be cracking your plate if you're hitting extremely hard (which is unnecessary) but it is very unlikely for the Antigen/antibody bonds to break.

@MonsieurLavabo 12 жыл бұрын

@MalagaJolu No, it really depends on the assay you are doing. For example it can be an antibody in Blocker Casein/PBS.

@danielegalati1039 9 жыл бұрын

excuse-me but after coating can we incubate at room temperature for 3 hours or 4°C overnight for wichever proteins? Or this incubation is specific for protein that you're working in this video?

@1993LAlice 8 жыл бұрын

What is the purpose of sealing the plate with parafilm? Thanks!

@assiadj7450 9 жыл бұрын

could u help me plz !! we need to know the cytotoxicity of Macrophages and PBMC which are stimulated with LPS,PMA/PHA .. so we used MTT test and after to detect absorbance, we used enzyme linked immunosorbent assay(ELISA) plate reader at 570... how we did this ELISA test !!??

@0102murphy 12 жыл бұрын

Will the coated antigen in the plate being shaked out if you hit the plate against the table so vigorously??

@shaymaaali6213 11 жыл бұрын

thank you

@thepoohbearful 11 жыл бұрын

NEED HELP ASAP! WHICH ELISA TEST SHOULD I USED TO CHECK THE PREVELANCE OF JOHNES DISEASE IN CATTLE?? ILL BE USING BLOOD / SERUM SAMPLES. AND WHAT KIT # ALSO?

@ArnavPadhi 4 жыл бұрын

Why antigen needs to be diluted in coating buffer??

@ManuelOrozcov003 12 жыл бұрын

OK well, but Cual es la función de cada reactivo utilizado en ese experimento? : )

@8t1c0f1s9h1 7 жыл бұрын

what is the name of the music?

@MalagaJolu 12 жыл бұрын

the coating buffer allways have to be 0.1 M Carbonate pH 9?

@justdeadnow1404 Жыл бұрын

11 years ago ? dayum

@benderadan Жыл бұрын

nice one very advantageous

@infoghinaztv5773 7 жыл бұрын

thanks . 👍👍

@joshj747 3 ай бұрын

Song is a banger a 2x speed

@Tepmodify Жыл бұрын

I like . Good technic

@martinmwandiki7000 9 жыл бұрын

I like the video

@shomeshali5675 5 жыл бұрын

Nice

@AkumaHaji 11 жыл бұрын

thx u for viedio . tomorrow i must do elisa T_T for project . this viedio i understand >^

@sohinisen7512 2 жыл бұрын

After coating antigen, why do you add the blocking buffer?

@mohammedahmedjalloh531 2 жыл бұрын

to prevent unspecified binding

@alexandrasoriano542 2 жыл бұрын

why does the song go crazy at 1.75x speed

@caleb8781 Жыл бұрын

Interesting

@SammyMageto-db7xw Жыл бұрын

By now Im able to know direct eliza

@MalagaJolu 12 жыл бұрын

Ok, for example I have my purificated Ag ( I want to perform a indirect ELISA) in Tris buffer 50 Mm, 100Mm Kcl, do u recomend me hibrid my antigen to the microplates with that buffer?