DNA cloning and recombinant DNA | Biomolecules | MCAT

DNA cloning and recombinant DNA | Biomolecules | MCAT | Khan Academy

Рет қаралды 1,073,211

8 жыл бұрын

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Introduction to DNA cloning.
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Пікірлер: 297

@samuelfrancis8574 3 жыл бұрын

I just realised.... these vids don’t have ads.... they still aren’t making no money off this.. god bless

@jessepalomares1427 2 жыл бұрын

yes thanks to not making any money we are successfully homeless .

@Ariadne4 2 жыл бұрын

What's wrong with making money off these tho? There are many useless videos making money off them, so why don't useful videos like these make money for the hardworking content creators?

@S3verance 2 жыл бұрын

Yeah, truely one of the best Not for profit organisations out there

@bladeiron Жыл бұрын

@@Ariadne4 He's not saying ads are bad, he's saying no ads are good

@sajidabrar1 Жыл бұрын

Live Wikipedia

@aileniirina3335 5 жыл бұрын

Sir, you are my favourite teacher on the Khan Academy platform. Great job !!! You helped me a lot during my studies

@emmanuelessel8650 Жыл бұрын

Thank you sir

@tarimandeliko2672 7 жыл бұрын

this is amazing. i have tried so hard to understand this (i have watched other videos, read an excerpt from a textbook etc.) and now i finally get it. thank you khan academy

@Unknowngamerborivt 5 жыл бұрын

how is he so smooth with the mouse?

@stitthappens 5 жыл бұрын

he is using a drawing tablet ! :)

@davisjohn1517 3 жыл бұрын

Bruh

@rundown3984 3 жыл бұрын

That’s what I thought

@samratgowda 3 жыл бұрын

Ohh, man he is using a writing pad.

@behindthecurrent9147 3 жыл бұрын

Experience *pen

@nicolasgobbi5620 3 ай бұрын

Even 7 years later, he's still the best teacher to ever walk this Earth. Thank you so much for helping me during my studies!

@JaseThePhoenix 5 жыл бұрын

I wish my professor did the same thing to their lectures, would save me time reading textbooks

@lucaslopez2091 3 жыл бұрын

you should never replace reading the textbook, these lessons are complementary since they are simplified.

@ekish9253 3 жыл бұрын

True i agree

@ghufran9635 2 жыл бұрын

THAT WAS AMAZING!!! For a moment I imagined you teach us in our university, it would be like a dream 😢 Thank youuuu so much

@unmceopcomplab3162 7 жыл бұрын

Great video, Khan Academy never fails to clear up difficult topics/processes.

@Marie-qv6on 3 жыл бұрын

who else has an exam in 2 days that they're desperately trying to cram for

@Fletcher-3rd 3 жыл бұрын

I have in the next 5 hours

@Marie-qv6on 3 жыл бұрын

got a 96 on the final

@rundown3984 3 жыл бұрын

@@Marie-qv6on congrats!

@miriamsthoughts 3 жыл бұрын

@@Marie-qv6on let's go! I have one tomorrow hopefully I'll understand the material well enough

@Darknight-dz6tp 3 жыл бұрын

@@miriamsthoughts how did it go😂?

@mydude9710 4 жыл бұрын

You can hear the passion he has for teaching science in his videos. Amazing job!

@uhm9361 7 ай бұрын

i know right frfrf

@jettburns8879 8 жыл бұрын

That's amazing, to think we can do this inside cells using biochemistry to get what we want! Keep them coming Sal, loving these new (micro)bio vids.

@youngspunky2835 7 жыл бұрын

gucci mane is a clone go look at him he is a human so yea do your research XD

@lucajones2349 4 жыл бұрын

This is genetics, not microbiology.

@AlunaScape 3 жыл бұрын

Umm i think to be precise its biotechnology-biomolecular.

@moskar2738 3 жыл бұрын

Such a great video!! Explained this concept so clearly!

@life9162 3 жыл бұрын

Who else reads the comments while sal is explaining 😂

@salamlababidi2644 3 жыл бұрын

Great video! Thank you, Sir. May I say that It's pretty amazing how you're fascinated by science 😂 you transferred that to us too!

@alperen3486 3 жыл бұрын

Bob Ross of biology :D

@daisyrivera9417 5 жыл бұрын

Thank you! I was reading my textbook and lecture notes, but still couldn’t understand it. This video explained everything perfectly 👌

@user-ys2ch1sd5i Жыл бұрын

Thank you for helping me through my lab courses. I would be lost without your videos.

@meredithgrey5316 3 жыл бұрын

WOOOOOOWWWW OMGG THANK YOOOUU I was watching all of my videos from uni and reading all of my lectures...didn't get a thing.....and you are here making it so easy, you literally save me from resit.

@raniahashim5400 3 жыл бұрын

SUPER helpful content - thank you SO much!

@eniamrahc73 3 жыл бұрын

Even in med school, you're still helping me. Thank you!

@paribhura6704 3 жыл бұрын

You are Doing this stuff in med school?

@eniamrahc73 3 жыл бұрын

@@paribhura6704 Lol unfortunately. We just started systems, but we covered this in our basic foundations 1st semester.

@paribhura6704 3 жыл бұрын

@@eniamrahc73 oh! We are doing a brief unit on it but I am in smiths grade, not many details

@weklihua 2 жыл бұрын

@@paribhura6704 just have to go back to this stuff again and again…..

@rotanux 2 жыл бұрын

Me too lol, first semester of med school, in 2 days I'll have my exam

@matteopradal5581 4 жыл бұрын

Very well done!! best explanation of this topic

@satishraina4434 2 жыл бұрын

Excellent Presentation. Very well done. The colours (against the dark background) make the intended impact.

@gladysdiekonifeoluwa4691 5 ай бұрын

You just simplified the bulky and complicated material given by my lecturer.. Thank you so much ❤🎉

@aquahot 3 жыл бұрын

Thank you Sal. I wish every teacher is as awesome as you are

@head4shot 3 жыл бұрын

This is the most concise and informative video on this.

@mohammedal-hammadi5085 5 жыл бұрын

You are awesome really, very clear and good video, thank you so much, we will wait for more videos

@CaroeJ 3 жыл бұрын

could you make a video and explain different kind if cloning? Gateway, Greengate, Goldenste are some examples I can't differentiate from each other...

@Lebrondagoat561 4 жыл бұрын

Got me through my assignment, thanks sir

@vickychen5984 4 жыл бұрын

make it easy to understand!! and it is almost identical to my lab materials but in simplify form!! Thank you!

@30vansan 5 жыл бұрын

Superb!! Very nice, simple explanation.

@malakgalhoum9907 11 ай бұрын

thank you very much i wasnt understanding it until you explained

@amandaz1454 3 жыл бұрын

started watching khan academy when i was in 9th grade and hi i'm in my first week of college turns out no professor teaches better than Khan :)

@0001-exe 3 жыл бұрын

Bro SAME! i also started watching since 9th grade and am now in my first year in college

@RaviGarlapati 3 жыл бұрын

Watched this video to understand what the term "DNA Cloning" means and how that's done with the help of recombinant DNA technology. Got to know that it's reproducing of the desirable genes/sections of DNA with the help of certain restriction enzymes, DNA ligase, and the host bacteria that supplies plasmids to attach the target genes and then reproduces them. This video also clarifies the question - "How to engineer microorganisms?" - for which I have seen other videos earlier (insulin production as a case in point)

@giftlesibatleanedemenaceth1649 Жыл бұрын

Which biotechnology techniques can be used to proof if certain cell express a particular enzyme? I wrote Elisa and Western blot. Pls help?

@smujb5544 6 жыл бұрын

this is good

@vijithanambuwasam8795 4 жыл бұрын

Thank you very much .finally I got it.

@letspractice9444 5 жыл бұрын

That's the exact way of teaching....loved it

@nikki49010 2 жыл бұрын

You make everything so much easier to understand

@mydream3914 6 жыл бұрын

Is there any videos about methods used to obtain DNA sequence?

@samsalla2381 5 жыл бұрын

The heat shock is happening through CaCl2, which is counteracting the electrostatic repulsion between plasmid DNA the bacterial cellular membrane ( liposaccharide layer), mostly E.Coli as it is most competent bacteriathan for these procedures.

@sriramsidartan690 4 жыл бұрын

Thankyou for the explanation

@ScienceGhetto Жыл бұрын

I really love khan because learning becomes easier.

@mariposacuriosa 4 жыл бұрын

Wonderful explanation!

@kierenrogers425 3 жыл бұрын

Great vid Sal, you're the man!

@-zl5go Жыл бұрын

Pro you are amazing person..really you explain any object i want to study it ..i don’t know how i can thank you 😢❤❤❤

@flipperdeflipperkast 6 жыл бұрын

thanks!!

@gregorysmith1134 2 жыл бұрын

Excellent lecturer. Great clarity.

@chloetaylor6789 4 жыл бұрын

Thank you for getting me through my studies! These videos really help piece it altogether. Much appreciated🙌 Your Northern Irish student😁

@kianhoover7650 3 жыл бұрын

great explanation! Thanks!!!

@mahendrahosalli4803 5 жыл бұрын

Tq so much for your kind information

@mariapaz8739 4 жыл бұрын

Thanks you have helped 😊

@elissar2004 Жыл бұрын

His videos are life saver!!!

@suhyu_n217 Жыл бұрын

와,, 이해하기 쉽게 설명 진짜 잘해주심 ㅠ

@gamestarz7030 4 жыл бұрын

Thank u sooooooo much . U saved a lot

@pirate1367 Жыл бұрын

You made my reading texbooks life easier

@jyothsna.m1053 5 жыл бұрын

Thank you so much

@AmiraMohamed-xr8dg 2 жыл бұрын

Great explanation. Thanks a lot.

@zienanema4691 4 жыл бұрын

Thank you very much your are agood teacher

@kluaywael 2 жыл бұрын

Thank you for the explantion🌻🌻

@syedmianzalaniqbalkakakhel7091 4 жыл бұрын

Well explained lecture Sir!!!!!!

@Ross.AA18 6 жыл бұрын

Explicas de lo mejor , se me quedó todo!!! gracias .

@NusratJahan-iz5rc 4 жыл бұрын

Thank you Khan academy ❤️

@lillyherbel2124 4 жыл бұрын

Thank you so much! I’m in biology D as a freshman right now, and with the whole online learning deal, I’m struggling in the class, and this made the whole idea wayyyy easier to understand! Thanks!

@gregsmith6756 Жыл бұрын

You would not even be doing this level of work as a freshman. It will probably be the first semester of your junior year before you start conducting these kinds of experiments.

@nitro.2405 Жыл бұрын

@@gregsmith6756 whats the point of responding 2 years later? lmao

@gokulgx007 11 ай бұрын

@@nitro.2405for others whom it may concern 😊

@ccopelandofficial 5 жыл бұрын

How do the proteins encoded by the new plasmid get expressed?

@Wabuh-Wabuh 4 жыл бұрын

What is the solution in the test tube made of?

@user-ze5do8ln7n 3 жыл бұрын

나 한국인인데 쌤 설명 지리네요

@mohamedsami5619 5 жыл бұрын

Thank youuu !!!!

@msms3737 2 жыл бұрын

thank you didnt understand what the overhangs were and overall process of DNA cloning

@stephenoclarke8703 3 жыл бұрын

excellent tutor!

@chaimataifi8799 2 жыл бұрын

Awesome explanation. Thanks a loooooooooooot.

@shroqhafed5717 3 жыл бұрын

Thank you 🙏

@marija347 3 жыл бұрын

How do we know for sure that the bacteria will express the genetic information from the plasmid, for example, even if the colony has taken up the plasmid, why would we be sure that antibiotic resistamce will be expressed?

@saiwashburn 5 жыл бұрын

Love the vids. Can you record at just a slightly higher volume please?

@nourm252 4 жыл бұрын

Man, thanks I love you.

@EclipZeMuzik 6 жыл бұрын

awesome video!!

@ManyHeavens42 Жыл бұрын

The first humans lived to be 900 years old now you see why its so important

@vincentdaly1766 3 жыл бұрын

谢谢

@hadeerrashad5486 5 жыл бұрын

Thank you so much for making this video!!!!

@JKSmith-qs2ii 6 жыл бұрын

I was wondering the following, this video tells me that DNA is taken from one organism and put into another organism's plasmid so that that organism can go and create a desired protein. My question is then, does the bacteria know how to splice the introns and exons when it's creating the desired protein. Much more logical to me would be that they take ripe mRNA, (already spliced correctly) reverse transcript that and put it in the plasmid, since bacteria don't have introns and exons.

@ayandamafunda312 5 жыл бұрын

Isn't bacteria a prokaryote and prokaryotes don't have introns.

@lahirusandaruwankarunaseka3944 4 жыл бұрын

Thank u. 😊

@thomasdeconinck1492 4 жыл бұрын

I am currently making a integrated test and man, you helped me a lot. Explanation is so good :)

@atisardias9622 3 жыл бұрын

thank you so much

@academictraveling 5 жыл бұрын

I'm 22. But can GOM work on whatever ages we are? or it only works for the embryo? haha.. as an example in my age 22, can I get blue eyes?

@Broskimania 5 жыл бұрын

brilliant

@rivu1943 4 жыл бұрын

Any Indians?

@Malikamali873 4 ай бұрын

Thank you so much ❤

@amirazimi2121 2 жыл бұрын

Thank you 💓

@lucindadewaal9511 4 жыл бұрын

Thank you thank you thank you

@user-yt4jm8wy8m 5 жыл бұрын

I can't sure that you respond to me, but I have a question. Is there any checking process that we can check the plasmid has a gene that we want to clone between 'Paste process' and 'Inserting the plasmid to the e.coli' ?

@aroojqureshi317 3 жыл бұрын

Nope..no way

@zoom-zip3473 Жыл бұрын

How do we compensate for the lost DNA fragment that was cut off for cloning?

@sadboibliss 8 ай бұрын

thank you 😃

@celestinennagbogu4415 2 жыл бұрын

Thank you

@pavan4147 3 жыл бұрын

I have a doubt sir. Like u will collect DNA fragment of a virus r plasmid of a higher organism then u will maintain it in such a organism within which u can easily insert a foreign DNa fragment Then which DNA acts as vector here N how many organisms DNA will be involved ..??

@GM.Nobody 4 жыл бұрын

the only good thing about youtube algorithm is that, it introduced me to Khan Academy

@sadaftagar220 Жыл бұрын

hello can u plx tell me from can i get DH5 Alpha e.coli? Do u have any link from where can i order it

@vahneeneenee686 4 жыл бұрын

ive struggled so much to understand this topic in my first language lol and now i tried to it in english and your video was so helpful (and pretty) thank yaa

@ayazurofficial3601 4 жыл бұрын

Thanks

@hollystoltzfus758 7 жыл бұрын

Which video describes the extraction of desired product? Insulin, for instance?

@elenanewton5056 7 жыл бұрын

Holly Stoltzfus

@daniellewebb9921 7 жыл бұрын

You would just add a restriction digest that flanks either side of the gene of interest.

@rogergr5019 3 жыл бұрын

I wonder how yoou look, you have a good radio voice

@allycat920 7 жыл бұрын

I am forever grateful to you because you are the ONLY REASON why I will pass my exam. Thank you thank you thank you!!!

@bt6611 5 жыл бұрын

This translates well in microbiology but how does an antibiotic resistant ecoli plasmid translate to animal species? You go into insulin but how does that work? Is it because we have altered the plasmid DNA which would render any ecoli effects useless?