Debunking Antibody Titration Myths
Рет қаралды 1,242
Жыл бұрынPresented By: Diana Bonilla
Speaker Biography: Diana studied Microbiology and Clinical Laboratory in Colombia. She is also an Immunologist, with a Ph.D. from Texas A&M University, a postdoctoral degree from Baylor College of Medicine and an ASCP-accreditation as a cytometry specialist. She has more than 20 years of experience as a biomedical scientist for a variety of applications, including infectious diseases and cancer, working as a senior scientist at MD Anderson Cancer Center.
Webinar: Debunking Antibody Titration Myths
Webinar Abstract: In this webinar, we will cover the fundamentals of antibody titrations, a critical step in the development and optimization of multicolor flow cytometry assays. We will discuss best practices and the validity of commonly held beliefs around antibody titrations.
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@jfromtheusa Жыл бұрын
References in talk: 04:00: Bradbury, A., Plückthun, A. Reproducibility: Standardize antibodies used in research. Nature 518, 27-29 (2015). doi.org/10.1038/518027a 04:00: Baker, M. Reproducibility crisis: Blame it on the antibodies. Nature 521, 274-276 (2015). doi.org/10.1038/521274a 04:00: Baker, M. When antibodies mislead: the quest for validation. September 2020: www.nature.com/articles/d41586-020-02549-1 04:05: Uhlen, M., Bandrowski, A., Carr, S. et al. A proposal for validation of antibodies. Nat Methods 13, 823-827 (2016). doi.org/10.1038/nmeth.3995 04:20: antibodypedia.com proteinatlas.org citeab.com antibodyregistry.org labome.com scicrunch.org/resources antibodies-online.com
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