Loop mediated isothermal amplification (LAMP protocol explained)

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Loop mediated isothermal amplification (LAMP protocol explained)

Рет қаралды 96,789

Күн бұрын

Dear Scientists,
LAMP, short for loop-mediated isothermal amplification, is an alternative technique/method for DNA amplification and has some advantages over PCR. The principle of the LAMP assay is based on a selection of 4 to 6 primers as shown as in this animated video*.
Best
Henrik
*Animation was done with Microsoft Office Powerpoint.
References:
www.ncbi.nlm.n...
Time Codes:
0:00 Introduction
0:37 LAMP vs PCR
1:19 LAMP principle
3:47 Outro

Пікірлер: 32

@user-pt3qo5nm9z 2 жыл бұрын

This is the clearest explanation I've found. THANK YOU!!

@CB-pi5hc 3 жыл бұрын

This was giving me a migraine and now i only have a small headache. Thank you for the help!

@henrikslab 3 жыл бұрын

Same here.. it was the reason why I did the video :D

@quackerzdb 2 жыл бұрын

How is each amplicon produced? How is the strand displacement accomplished, is it a function of the polymerase? Why is the loop formation necessary?

@sagargautam7015 Жыл бұрын

Although it seems simple, it's difficult to understand.. You made it really easy. Thank you for the explanation. :)

@bakeology9013 Жыл бұрын

How both DNA denaturation and primer binding is occuring at same temperature?As both of them are opposite reaction?

@asmaaghazaal9798 Жыл бұрын

the most clear explain .. thanks

@kunhuilee5672 2 жыл бұрын

Thank you for your video. i praise you to talk slowly and good pronunciation!! i'm about to subscribe your channel.

@chrisgeorge8553 2 жыл бұрын

Loved this explaination!!!

@marchielumsy1673 2 жыл бұрын

Heyy, I did LAMP today. The experimental setup was pretty straightforward. BUT, trying to understand the principles behind it, as concisely explained in your video is quite a challenge for me to wrap my head around it. I'll have to do more reading for sure.. 😂 Thanks for the explanation!

@sadnesssarcoma7359 Жыл бұрын

Hello, I need your help.... ahaha

@iqrakhalil6693 Жыл бұрын

Can u explain me plz

@kassialorrany1875 2 жыл бұрын

Very good!!!! Thank you!

@mariociencia12 3 жыл бұрын

Excellent! Finally, I understood the loop structure formation. However, how does it makes the DNA duplication faster?

@henrikslab 3 жыл бұрын

In PCR there are 3 cycles which combined take up some time to generate the daughter strands. In LAMP the synthesis continues constantly.. further: using 6 primers in case of LAMP, provides more contacts for polymerase recruitment!

@TheEdtechgirl 11 ай бұрын

Hi there. Is there any way you can turn on captions for this video? Thank you

@shabnamnaderifar Жыл бұрын

perfect , thank you

@mariociencia12 3 жыл бұрын

Valeu!

@mariociencia12 3 жыл бұрын

The animation is very helpful. It will be even better if was shown at least one cycle after the annealing of external primers, indicated in 3:14. Unfortunately, I live in Brazil and my financial situation is not so good as I would like, but your animations deserve much more than $ 2.00. And thank you for answering my questions!

@henrikslab 3 жыл бұрын

@@mariociencia12 Why do you donate, when your financial situation is not so good? I am extremely thankful, but I have to make that clear: I would not want anyone to donate if they have to keep an eye on their money!! Thanks anyways :) *And you´re right - I spared myself the animation of the cycle

@mariociencia12 3 жыл бұрын

@@henrikslab I would like to donate more! You deserve! But the donation that I did will do not compromise me. Do not worry. I also did it as an example, hoping that other viewers do the same

@henrikslab 3 жыл бұрын

@@mariociencia12 That´s very kind of you! But it helps me also so much when people like, subscribe and share the channel with friends!

@tumpiliksye 3 жыл бұрын

Like and subscribed. Very good job 👏👍

@NguyenMinh-rd3fx 8 ай бұрын

I read that most DNA denatured at 75 degrees, does the DNA denatured at 60-65 degrees in LAMP or just some location? Can this become a limitation of LAMP?

@qiaofengli1100 3 жыл бұрын

great👍👍👍

@motaharehjadidi5875 3 жыл бұрын

perfect!

@louisaelenar4523 8 ай бұрын

Why do you only have the first product after the steps at 2:50. I thought the very first product is the one at 2:00 ?

@carmenmayoral6744 Жыл бұрын

I don't know how the first strand invasion occurs

@ezrahyazmenecalixtro9744 8 ай бұрын

yey

@julianpapa6556 Жыл бұрын

capooooooooooooooooooooo

@MrHabaHH 3 жыл бұрын

Thanks for the work that you put in. Hit me a massage if you like. I am a master in biochemistry and molecular biology maybe we can arrange something together.