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Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates - a 2 minute Preview of the Experimental Protocol
Min Zhu, Sepideh Heydarkhan-Hagvall, Marc Hedrick, Prosper Benhaim, Patricia Zuk
Cytori Therapeutics Inc,; David Geffen School of Medicine at UCLA, Division of Cardiac Surgery, Department of Surgery; David Geffen School of Medicine at UCLA, Division of Plastic and Reconstructive Surgery, Department of Surgery; David Geffen School of Medicine at UCLA, Department of Orthopedic Surgery; David Geffen School of Medicine at UCLA, Regenerative Bioengineering and Repair Laboratory;
In 2001, researchers at UCLA described the isolation of a population of adult stem cells, termed Adipose-derived Stem Cells or ASCs, from adipose tissue. This article outlines the isolation of ASCs from lipoaspirates using a manual, enzymatic digestion protocol using collagenase.
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