DP will vary from compound to compound. MCA just ensures a good average is obtained and reduces variation in apex of peak

You must use MS grade solvents only and filter through 0.22um membranes ideally. This avoids particulates. Where is the machine located? People at your lab who know how to run these instruments forget how challenging it can be to become proficient.

We are so happy that you found this video helpful! Thank you for that:) I reached out to our partners at SCIEX regarding your question. Here is their reply: "With high res instruments, most people don’t really do tuning in the same way as for QqQ instruments. There are a couple of reasons for this: 1. Usually HR instruments are used for more qualitative work, so the quantitative needs are not as great 2. Generally quant is done from the ToF scan, so fragmentation is not used for quant (although it can be!) If someone wants to do HRMS quant in a way similar to a QqQ, what we call MRM-HR, the tuning process is similar. They need to ramp collision energy to get efficient fragmentation. It is a little different because there will be one CE setting to generate and monitor multiple fragments, not a CE for each MRM. Other parts of the question are a bit confusing, I’m not sure how to interpret." Here’s a video link explaining the scan. sciex.com/community/application-discussions/technology/qtof/fundamentals/multiple-reaction-monitoring-high-resolution-mrm-hr-scan-function Hope this was helpful!

Hello! Unfortunately, the scientist who starred in that video no longer works in our lab. However, SCIEX does recommend 5-10ul/min. You can find their full manual here sciex.com/x51122. I hope that helps! However, if it doesn't, please reach out to our technical experts at Live Chat at www.phenomenex.com/chat.