MEGA X: How to construct a phylogenetic tree

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MEGA X: How to construct a phylogenetic tree

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Amer Al-Jawabreh

Күн бұрын

Пікірлер: 57

@kakakycj480 8 күн бұрын

Thank you so much, Your video very good.

@alexsavesharks 8 ай бұрын

The music makes this sound like a motivational video😂, probably fitting. Need David Goggins to narrate this XD

@7oz_latte 26 күн бұрын

Wonderful teaching ！ So easy and comprehensive ！！

@fatimafellah9047 3 жыл бұрын

thank you so much; you save my life because your video was so easy and comprehensive

@mouthescientist4949 2 жыл бұрын

Thanks to your video I made my first phylogenetic tree

@ameral-jawabreh9778 2 жыл бұрын

Good luck

@W4VDragon 7 ай бұрын

Thank you! Very simple and informative tutorial! 👍

@aftabnadim 2 жыл бұрын

Thanks so much. Very simple and to the point video.

@sanniajaved3249 2 жыл бұрын

Extraordinary video sir . Jazak Allah

@ameral-jawabreh9778 2 жыл бұрын

Goal is to simplify research

@cessy6293 2 жыл бұрын

Thank you so much for the simple explanation!

@suzyeshukri4332 2 жыл бұрын

very usefull video, thanks a lot my friend.

@tnfsf10 6 ай бұрын

Ambience is so epic 😁

@rubensdiogo9975 2 жыл бұрын

Nice class! Thanks for it...

@samanthaclark9497 2 жыл бұрын

Excellent video, also I love the music!

@moulaymhammedakermi3410 2 жыл бұрын

THANK YOU FREIND

@wks6034 3 жыл бұрын

Hello friend. I found your video very usefull. One tiny recommendation: The music in the background could be optimized. Thank you for sharing your knowledge.

@nnabuikeogbonna5565 2 жыл бұрын

Thanks so much. It was quite useful.

@ramonoYT Жыл бұрын

Thanks! it worked just fine

@ameral-jawabreh9778 Жыл бұрын

Nice to hear that

@manishkatiyar2649 2 жыл бұрын

thank u sir

@Basedgemax 8 ай бұрын

epic

@nohataha6398 Жыл бұрын

هل يا دكتور لو بابحث باستخدام blast ووجدت ان الجين مقارب فقط بنسبه 70% من السلالات المقترحه وليس 99% او 100% فمامعنى حوث هذا؟؟؟شكرا مقدما لحضرتك

@ameral-jawabreh9778 Жыл бұрын

70% similarity is weak. It means that your sequence does not match any sequence in the GenBank. Perhaps there was a problem with your sequence. I will not build my analysis on such a low similarity percentage

@karensegura6234 2 жыл бұрын

great video, bad background music. too distracting.

@sumitkumar-el3kc 2 жыл бұрын

Can you please tell me how to display the bootstrap value? I did select the bootstrap value of 1000 but didn't get it displayed on the tree.

@soohyun704 Жыл бұрын

I heard that the front and rear sequences should be cut to match the length of the sequences after alignment. Is that process unnecessary?

@ameral-jawabreh9778 Жыл бұрын

Sometimes Sanger sequences have troubles in their flanking regions, only then we can trim these regions. Better to do your tree with and without trimming and see which one is more logical

@soohyun704 Жыл бұрын

Thanks for the heads-up!

@bipinkb8519 2 жыл бұрын

The video was very informative, I have used this same procedure and at last when I use MUSCLE->Align DNA, it shows like "Application Error finalizing MUSCLE alignment: Unable to open file" why it is showing like this, can you help me sir?

@afiagul6332 2 жыл бұрын

same problem i faced. then i just went to that specific site of sequence(shown in number in error pop-up) and then just replaced '?' (for 'N' nucleotides) with '-' and it worked.

@saradapaudel6015 Жыл бұрын

Sir can you please make videos on how to use spss for associated risk factor of intestinal parasitic infection using logistic regression method?? Please...i am so much confused to use bivariate multinomial regression

@ameral-jawabreh9778 Жыл бұрын

This is very important in research. You may refer to one of my publications: pubmed.ncbi.nlm.nih.gov/31829156/

@casualsatanist5808 Жыл бұрын

Im having a midlife crisis listening to this beautifully depressive music

@kchaitanya5116 3 жыл бұрын

How to allign protein sequences.?

@nohataha6398 Жыл бұрын

هل من الافضل انى لما اعمل submission for my seq اختار انى اختار مده مثلا 7 اشهر حتى انشر شغلى فى مجله ولا اعمل immediate release? على gene bank?? شكرا لحضرتك يا دكتور

@ameral-jawabreh9778 Жыл бұрын

Immediate

@kushankursarkar6222 2 жыл бұрын

Sir how can i construct a phylogenetic tree from phytochemicals using mega?

@joannalynmontemayor3094 3 жыл бұрын

Hi! Just wondering where can I trim my alligment from starting and end region.

@ameral-jawabreh9778 3 жыл бұрын

There are softwares that help in trimming. Trimming can be done by removing areas with extensive Ns. Replace the N with (?). Remove the beginning and the ends of sequences that extend beyond your reference strain or target area. I once built a tree with and without trimming. Both were almost similar. Of course, you have to use reasonable sequences which do not have many Ns.

@eukaryotic0703 Жыл бұрын

You remind me of Dr Zola from Avengers

@user-lb9fx9gf1i 2 жыл бұрын

Thank you so much sir for this useful video, I met a problem in the last step for creating the tree did not appear and an error was found (no common sites found for computing distances.....)

@jamking2448 2 жыл бұрын

Same what Did u do for that

@jamking2448 2 жыл бұрын

Kindly reply as fast as possible because I have to submit it

@eukaryotic0703 Жыл бұрын

please, what is the song playing ?

@eukaryotic0703 Жыл бұрын

Journey beyond the stars.... the music is a really nice touch to the video

@keityfarfan1472 3 жыл бұрын

Hi, and thanks for the video. Please, I need help, because I did the alignments with muscle and I saved it in mega format. But when I need to do the phylogenetic tree, appear an error that says: "Aligned sequences must be equal lengths". How can I solve this? Thanks!

@ameral-jawabreh9778 3 жыл бұрын

I never had this problem despite using sequences with variable lengths. Maybe the lengths of your sequences are extremely variable.

@saqibaagha7665 3 жыл бұрын

Delete extra sequences

@afiagul6332 2 жыл бұрын

make them equal in length by adding ----------